Erythropoietin production by macrophages in the regenerating liver
The site of erythropoietin (Ep) production and/or storage in the rat liver was determined. A guinea pig anti‐Ep was produced against purified rat Ep (64,096 ± 4J064 IU/mg). This antibody was found to be highly specific using rocket immunoelectrophoresis, Ouchterlony gel diffusion methods, and immuno...
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| Veröffentlicht in: | Journal of surgical oncology 1985-11, Vol.30 (3), p.184-197 |
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| container_title | Journal of surgical oncology |
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| creator | Naughton, Gail K. Naughton, Brian A. Gordon, A. S. |
| description | The site of erythropoietin (Ep) production and/or storage in the rat liver was determined. A guinea pig anti‐Ep was produced against purified rat Ep (64,096 ± 4J064 IU/mg). This antibody was found to be highly specific using rocket immunoelectrophoresis, Ouchterlony gel diffusion methods, and immunoprecipitin reactions as well as Ep neutralization tests (capable of completely neutralizing up to 2,000 IU Ep/mg). This anti‐Ep was labeled with either fluorescein for light microscopic study or ferritin for electron microscopy. Kupffer cells showed varying degrees of labeling after hepatectomy alone or hepatectomy combined with nephrectomy and/or hypoxia. Greatest labeling was seen in Kupffer cells of rats that were nephrectomized 48 hr posthepatectomy and kept at ambient pressure. No labeling of hepatocytes or vascular and bile duct endothelium was noted. |
| doi_str_mv | 10.1002/jso.2930300312 |
| format | Article |
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S.</creator><creatorcontrib>Naughton, Gail K. ; Naughton, Brian A. ; Gordon, A. S.</creatorcontrib><description>The site of erythropoietin (Ep) production and/or storage in the rat liver was determined. A guinea pig anti‐Ep was produced against purified rat Ep (64,096 ± 4J064 IU/mg). This antibody was found to be highly specific using rocket immunoelectrophoresis, Ouchterlony gel diffusion methods, and immunoprecipitin reactions as well as Ep neutralization tests (capable of completely neutralizing up to 2,000 IU Ep/mg). This anti‐Ep was labeled with either fluorescein for light microscopic study or ferritin for electron microscopy. Kupffer cells showed varying degrees of labeling after hepatectomy alone or hepatectomy combined with nephrectomy and/or hypoxia. Greatest labeling was seen in Kupffer cells of rats that were nephrectomized 48 hr posthepatectomy and kept at ambient pressure. No labeling of hepatocytes or vascular and bile duct endothelium was noted.</description><identifier>ISSN: 0022-4790</identifier><identifier>EISSN: 1096-9098</identifier><identifier>DOI: 10.1002/jso.2930300312</identifier><identifier>PMID: 3935875</identifier><identifier>CODEN: JSONAU</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Antibodies - analysis ; Antibodies - metabolism ; antierythropoietin ; Biological and medical sciences ; erythropoietin ; Erythropoietin - biosynthesis ; Erythropoietin - immunology ; Erythropoietin - isolation & purification ; Female ; ferritin ; Ferritins - immunology ; fluorescein ; Fluorescein-5-isothiocyanate ; Fluoresceins - immunology ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Hypoxia - metabolism ; Kupffer cell ; Kupffer Cells - analysis ; Kupffer Cells - metabolism ; Kupffer Cells - ultrastructure ; liver ; Liver Regeneration ; Liver. Bile. Biliary tracts ; Macrophages - analysis ; Macrophages - metabolism ; Male ; Rats ; Rats, Inbred Strains ; Thiocyanates - immunology ; Vertebrates: digestive system</subject><ispartof>Journal of surgical oncology, 1985-11, Vol.30 (3), p.184-197</ispartof><rights>Copyright © 1985 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4392-4c868e0862e284b3dd262467eec88fff3ee9918609bcb993d89d50a814ad5f513</citedby><cites>FETCH-LOGICAL-c4392-4c868e0862e284b3dd262467eec88fff3ee9918609bcb993d89d50a814ad5f513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjso.2930300312$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjso.2930300312$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27913,27914,45563,45564</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8568467$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3935875$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Naughton, Gail K.</creatorcontrib><creatorcontrib>Naughton, Brian A.</creatorcontrib><creatorcontrib>Gordon, A. S.</creatorcontrib><title>Erythropoietin production by macrophages in the regenerating liver</title><title>Journal of surgical oncology</title><addtitle>J. Surg. Oncol</addtitle><description>The site of erythropoietin (Ep) production and/or storage in the rat liver was determined. A guinea pig anti‐Ep was produced against purified rat Ep (64,096 ± 4J064 IU/mg). This antibody was found to be highly specific using rocket immunoelectrophoresis, Ouchterlony gel diffusion methods, and immunoprecipitin reactions as well as Ep neutralization tests (capable of completely neutralizing up to 2,000 IU Ep/mg). This anti‐Ep was labeled with either fluorescein for light microscopic study or ferritin for electron microscopy. Kupffer cells showed varying degrees of labeling after hepatectomy alone or hepatectomy combined with nephrectomy and/or hypoxia. Greatest labeling was seen in Kupffer cells of rats that were nephrectomized 48 hr posthepatectomy and kept at ambient pressure. No labeling of hepatocytes or vascular and bile duct endothelium was noted.</description><subject>Animals</subject><subject>Antibodies - analysis</subject><subject>Antibodies - metabolism</subject><subject>antierythropoietin</subject><subject>Biological and medical sciences</subject><subject>erythropoietin</subject><subject>Erythropoietin - biosynthesis</subject><subject>Erythropoietin - immunology</subject><subject>Erythropoietin - isolation & purification</subject><subject>Female</subject><subject>ferritin</subject><subject>Ferritins - immunology</subject><subject>fluorescein</subject><subject>Fluorescein-5-isothiocyanate</subject><subject>Fluoresceins - immunology</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Hypoxia - metabolism</subject><subject>Kupffer cell</subject><subject>Kupffer Cells - analysis</subject><subject>Kupffer Cells - metabolism</subject><subject>Kupffer Cells - ultrastructure</subject><subject>liver</subject><subject>Liver Regeneration</subject><subject>Liver. Bile. Biliary tracts</subject><subject>Macrophages - analysis</subject><subject>Macrophages - metabolism</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Thiocyanates - immunology</subject><subject>Vertebrates: digestive system</subject><issn>0022-4790</issn><issn>1096-9098</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9P2zAcxa2JCUq3KzekHBCc0tlx7Ph7BMSvqRuTxgbiYjnON60hTYqdwvrfz6hVEZft5MP7vOf3fYTsMTpilGZfHkI3yoBTTiln2QcyYBRkChTUFhlEIEvzAugO2Q3hgVIKIPNtss2BC1WIATk588t-6rt557B3bTL3XbWwvevapFwmM2OjNDUTDEkU-ykmHifYojcRniSNe0b_iXysTRPw8_odkl_nZzenl-n4-uLq9Hic2pxDrGGVVEiVzDBTecmrKpNZLgtEq1Rd1xwRgClJobQlAK8UVIIaxXJTiVowPiSHq9zY8WmBodczFyw2jWmxWwRdSMFFAa_g0b9BkQvJGYNIjlZkPDMEj7WeezczfqkZ1a_z6jivfps3GvbX0YtyhtUGX-8Z9YO1boI1Te1Na13YYEpIFU-OGKywF9fg8j-f6q8_r99VSFdeF3r8s_Ea_6hjciH07fcLfQ73xbcfv-_0Df8LNOCiUQ</recordid><startdate>198511</startdate><enddate>198511</enddate><creator>Naughton, Gail K.</creator><creator>Naughton, Brian A.</creator><creator>Gordon, A. S.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>198511</creationdate><title>Erythropoietin production by macrophages in the regenerating liver</title><author>Naughton, Gail K. ; Naughton, Brian A. ; Gordon, A. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4392-4c868e0862e284b3dd262467eec88fff3ee9918609bcb993d89d50a814ad5f513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Antibodies - analysis</topic><topic>Antibodies - metabolism</topic><topic>antierythropoietin</topic><topic>Biological and medical sciences</topic><topic>erythropoietin</topic><topic>Erythropoietin - biosynthesis</topic><topic>Erythropoietin - immunology</topic><topic>Erythropoietin - isolation & purification</topic><topic>Female</topic><topic>ferritin</topic><topic>Ferritins - immunology</topic><topic>fluorescein</topic><topic>Fluorescein-5-isothiocyanate</topic><topic>Fluoresceins - immunology</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Hypoxia - metabolism</topic><topic>Kupffer cell</topic><topic>Kupffer Cells - analysis</topic><topic>Kupffer Cells - metabolism</topic><topic>Kupffer Cells - ultrastructure</topic><topic>liver</topic><topic>Liver Regeneration</topic><topic>Liver. Bile. Biliary tracts</topic><topic>Macrophages - analysis</topic><topic>Macrophages - metabolism</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Thiocyanates - immunology</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naughton, Gail K.</creatorcontrib><creatorcontrib>Naughton, Brian A.</creatorcontrib><creatorcontrib>Gordon, A. S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of surgical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naughton, Gail K.</au><au>Naughton, Brian A.</au><au>Gordon, A. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Erythropoietin production by macrophages in the regenerating liver</atitle><jtitle>Journal of surgical oncology</jtitle><addtitle>J. Surg. Oncol</addtitle><date>1985-11</date><risdate>1985</risdate><volume>30</volume><issue>3</issue><spage>184</spage><epage>197</epage><pages>184-197</pages><issn>0022-4790</issn><eissn>1096-9098</eissn><coden>JSONAU</coden><abstract>The site of erythropoietin (Ep) production and/or storage in the rat liver was determined. A guinea pig anti‐Ep was produced against purified rat Ep (64,096 ± 4J064 IU/mg). This antibody was found to be highly specific using rocket immunoelectrophoresis, Ouchterlony gel diffusion methods, and immunoprecipitin reactions as well as Ep neutralization tests (capable of completely neutralizing up to 2,000 IU Ep/mg). This anti‐Ep was labeled with either fluorescein for light microscopic study or ferritin for electron microscopy. Kupffer cells showed varying degrees of labeling after hepatectomy alone or hepatectomy combined with nephrectomy and/or hypoxia. Greatest labeling was seen in Kupffer cells of rats that were nephrectomized 48 hr posthepatectomy and kept at ambient pressure. No labeling of hepatocytes or vascular and bile duct endothelium was noted.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>3935875</pmid><doi>10.1002/jso.2930300312</doi><tpages>14</tpages></addata></record> |
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| subjects | Animals Antibodies - analysis Antibodies - metabolism antierythropoietin Biological and medical sciences erythropoietin Erythropoietin - biosynthesis Erythropoietin - immunology Erythropoietin - isolation & purification Female ferritin Ferritins - immunology fluorescein Fluorescein-5-isothiocyanate Fluoresceins - immunology Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Guinea Pigs Hypoxia - metabolism Kupffer cell Kupffer Cells - analysis Kupffer Cells - metabolism Kupffer Cells - ultrastructure liver Liver Regeneration Liver. Bile. Biliary tracts Macrophages - analysis Macrophages - metabolism Male Rats Rats, Inbred Strains Thiocyanates - immunology Vertebrates: digestive system |
| title | Erythropoietin production by macrophages in the regenerating liver |
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