Lymphocyte adhesion to brain capillary endothelial cells in vitro
The presence and upregulation of adhesion molecules on bovine brain endothelial cells (BBEC) were investigated. Monolayers of BBEC were incubated with lipopolysaccharide (LPS), interleukin-1β (rhIL-1β), and interleukin-6 (rhIL-6) to simulate in vitro an inflammatory site in the cerebral capillaries....
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| Veröffentlicht in: | Journal of neuroimmunology 1994-06, Vol.52 (1), p.1-8 |
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| container_title | Journal of neuroimmunology |
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| creator | de Vries, Helga E. Moor, Anne C.E. Blom-Roosemalen, Margret C.M. de Boer, Albertus G. Breimer, Douwe D. van Berkel, Theo J.C. Kuiper, Johan |
| description | The presence and upregulation of adhesion molecules on bovine brain endothelial cells (BBEC) were investigated. Monolayers of BBEC were incubated with lipopolysaccharide (LPS), interleukin-1β (rhIL-1β), and interleukin-6 (rhIL-6) to simulate in vitro an inflammatory site in the cerebral capillaries. Adhesion of lymphocytes to BBEC increased 4.1-fold after stimulation of the endothelial cells for 4 h with 5 or 10 ng/ml LPS. Lymphocyte adhesion increased after incubation of the BBEC for 4 h with IL-1 and was increased 3.7-fold using 100 ng/ml IL-1. BBEC pre-incubated with IL-6 for 4 h also showed an increase in adhesion of lymphocytes, and cells pretreated with 100 ng/ml IL-6 showed a 3-fold increase in lymphocyte adherence. Specific monoclonal antibodies directed against CD11a, CD18, and VLA-4 were able to block adherence of lymphocytes to stimulated BBEC. These results indicate that the in vitro activation of BBEC may serve as a model for the study of inflammation of the blood-brain barrier. |
| doi_str_mv | 10.1016/0165-5728(94)90155-4 |
| format | Article |
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Monolayers of BBEC were incubated with lipopolysaccharide (LPS), interleukin-1β (rhIL-1β), and interleukin-6 (rhIL-6) to simulate in vitro an inflammatory site in the cerebral capillaries. Adhesion of lymphocytes to BBEC increased 4.1-fold after stimulation of the endothelial cells for 4 h with 5 or 10 ng/ml LPS. Lymphocyte adhesion increased after incubation of the BBEC for 4 h with IL-1 and was increased 3.7-fold using 100 ng/ml IL-1. BBEC pre-incubated with IL-6 for 4 h also showed an increase in adhesion of lymphocytes, and cells pretreated with 100 ng/ml IL-6 showed a 3-fold increase in lymphocyte adherence. Specific monoclonal antibodies directed against CD11a, CD18, and VLA-4 were able to block adherence of lymphocytes to stimulated BBEC. These results indicate that the in vitro activation of BBEC may serve as a model for the study of inflammation of the blood-brain barrier.</description><identifier>ISSN: 0165-5728</identifier><identifier>EISSN: 1872-8421</identifier><identifier>DOI: 10.1016/0165-5728(94)90155-4</identifier><identifier>PMID: 7911476</identifier><identifier>CODEN: JNRIDW</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Antigens, CD - physiology ; Biological and medical sciences ; Blood-Brain Barrier ; Brain - blood supply ; Capillaries - cytology ; Cattle ; CD18 Antigens ; Cell Adhesion ; Cell Adhesion Molecules - physiology ; Cells, Cultured ; Cerebral endothelial cells ; Endothelium, Vascular - cytology ; Endothelium, Vascular - physiology ; Fundamental and applied biological sciences. Psychology ; Inflammation ; Intercellular Adhesion Molecule-1 ; Interleukin ; Lipopolysaccharide ; Lipopolysaccharides - pharmacology ; Lymphocyte Function-Associated Antigen-1 - physiology ; Lymphocytes - physiology ; Molecular and cellular biology ; Receptors, Very Late Antigen - physiology</subject><ispartof>Journal of neuroimmunology, 1994-06, Vol.52 (1), p.1-8</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-dcc67d171c91642eff0bc438936892e911846969878fbb2dbe902d629bfe8a623</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0165-5728(94)90155-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4147955$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7911476$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Vries, Helga E.</creatorcontrib><creatorcontrib>Moor, Anne C.E.</creatorcontrib><creatorcontrib>Blom-Roosemalen, Margret C.M.</creatorcontrib><creatorcontrib>de Boer, Albertus G.</creatorcontrib><creatorcontrib>Breimer, Douwe D.</creatorcontrib><creatorcontrib>van Berkel, Theo J.C.</creatorcontrib><creatorcontrib>Kuiper, Johan</creatorcontrib><title>Lymphocyte adhesion to brain capillary endothelial cells in vitro</title><title>Journal of neuroimmunology</title><addtitle>J Neuroimmunol</addtitle><description>The presence and upregulation of adhesion molecules on bovine brain endothelial cells (BBEC) were investigated. Monolayers of BBEC were incubated with lipopolysaccharide (LPS), interleukin-1β (rhIL-1β), and interleukin-6 (rhIL-6) to simulate in vitro an inflammatory site in the cerebral capillaries. Adhesion of lymphocytes to BBEC increased 4.1-fold after stimulation of the endothelial cells for 4 h with 5 or 10 ng/ml LPS. Lymphocyte adhesion increased after incubation of the BBEC for 4 h with IL-1 and was increased 3.7-fold using 100 ng/ml IL-1. BBEC pre-incubated with IL-6 for 4 h also showed an increase in adhesion of lymphocytes, and cells pretreated with 100 ng/ml IL-6 showed a 3-fold increase in lymphocyte adherence. Specific monoclonal antibodies directed against CD11a, CD18, and VLA-4 were able to block adherence of lymphocytes to stimulated BBEC. These results indicate that the in vitro activation of BBEC may serve as a model for the study of inflammation of the blood-brain barrier.</description><subject>Animals</subject><subject>Antigens, CD - physiology</subject><subject>Biological and medical sciences</subject><subject>Blood-Brain Barrier</subject><subject>Brain - blood supply</subject><subject>Capillaries - cytology</subject><subject>Cattle</subject><subject>CD18 Antigens</subject><subject>Cell Adhesion</subject><subject>Cell Adhesion Molecules - physiology</subject><subject>Cells, Cultured</subject><subject>Cerebral endothelial cells</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Inflammation</subject><subject>Intercellular Adhesion Molecule-1</subject><subject>Interleukin</subject><subject>Lipopolysaccharide</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Lymphocyte Function-Associated Antigen-1 - physiology</subject><subject>Lymphocytes - physiology</subject><subject>Molecular and cellular biology</subject><subject>Receptors, Very Late Antigen - physiology</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1LAzEURYMotVb_gcIsRHQxOslk8rERSvELCm50HTLJGxqZTmoyLfTfm7GlS3ERsrjnPe47CF3i4h4XmD2kV-UVJ-JW0jtZ4KrK6REaY8FJLijBx2h8QE7RWYxfRYJKKkdoxCXGlLMxms63y9XCm20PmbYLiM53We-zOmjXZUavXNvqsM2gs75fQOt0mxlo25ileOP64M_RSaPbCBf7f4I-n58-Zq_5_P3lbTad54aKss-tMYxbzLGRmFECTVPUhpZClkxIAqmPoEwyKbho6prYGmRBLCOybkBoRsoJutntXQX_vYbYq6WLQxXdgV9HxVlFePkPEPOiopwOIN2BJvgYAzRqFdwyXatwoQbFavCnBn9KUvWrWNE0drXfv66XYA9De6cpv97nOhrdNkF3xsUDRhMkqyphjzsMkrSNg6CicdAZsC6A6ZX17u8eP5HAlp4</recordid><startdate>19940601</startdate><enddate>19940601</enddate><creator>de Vries, Helga E.</creator><creator>Moor, Anne C.E.</creator><creator>Blom-Roosemalen, Margret C.M.</creator><creator>de Boer, Albertus G.</creator><creator>Breimer, Douwe D.</creator><creator>van Berkel, Theo J.C.</creator><creator>Kuiper, Johan</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19940601</creationdate><title>Lymphocyte adhesion to brain capillary endothelial cells in vitro</title><author>de Vries, Helga E. ; Moor, Anne C.E. ; Blom-Roosemalen, Margret C.M. ; de Boer, Albertus G. ; Breimer, Douwe D. ; van Berkel, Theo J.C. ; Kuiper, Johan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-dcc67d171c91642eff0bc438936892e911846969878fbb2dbe902d629bfe8a623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Antigens, CD - physiology</topic><topic>Biological and medical sciences</topic><topic>Blood-Brain Barrier</topic><topic>Brain - blood supply</topic><topic>Capillaries - cytology</topic><topic>Cattle</topic><topic>CD18 Antigens</topic><topic>Cell Adhesion</topic><topic>Cell Adhesion Molecules - physiology</topic><topic>Cells, Cultured</topic><topic>Cerebral endothelial cells</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Inflammation</topic><topic>Intercellular Adhesion Molecule-1</topic><topic>Interleukin</topic><topic>Lipopolysaccharide</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Lymphocyte Function-Associated Antigen-1 - physiology</topic><topic>Lymphocytes - physiology</topic><topic>Molecular and cellular biology</topic><topic>Receptors, Very Late Antigen - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Vries, Helga E.</creatorcontrib><creatorcontrib>Moor, Anne C.E.</creatorcontrib><creatorcontrib>Blom-Roosemalen, Margret C.M.</creatorcontrib><creatorcontrib>de Boer, Albertus G.</creatorcontrib><creatorcontrib>Breimer, Douwe D.</creatorcontrib><creatorcontrib>van Berkel, Theo J.C.</creatorcontrib><creatorcontrib>Kuiper, Johan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Vries, Helga E.</au><au>Moor, Anne C.E.</au><au>Blom-Roosemalen, Margret C.M.</au><au>de Boer, Albertus G.</au><au>Breimer, Douwe D.</au><au>van Berkel, Theo J.C.</au><au>Kuiper, Johan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lymphocyte adhesion to brain capillary endothelial cells in vitro</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>1994-06-01</date><risdate>1994</risdate><volume>52</volume><issue>1</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><coden>JNRIDW</coden><abstract>The presence and upregulation of adhesion molecules on bovine brain endothelial cells (BBEC) were investigated. Monolayers of BBEC were incubated with lipopolysaccharide (LPS), interleukin-1β (rhIL-1β), and interleukin-6 (rhIL-6) to simulate in vitro an inflammatory site in the cerebral capillaries. Adhesion of lymphocytes to BBEC increased 4.1-fold after stimulation of the endothelial cells for 4 h with 5 or 10 ng/ml LPS. Lymphocyte adhesion increased after incubation of the BBEC for 4 h with IL-1 and was increased 3.7-fold using 100 ng/ml IL-1. BBEC pre-incubated with IL-6 for 4 h also showed an increase in adhesion of lymphocytes, and cells pretreated with 100 ng/ml IL-6 showed a 3-fold increase in lymphocyte adherence. Specific monoclonal antibodies directed against CD11a, CD18, and VLA-4 were able to block adherence of lymphocytes to stimulated BBEC. These results indicate that the in vitro activation of BBEC may serve as a model for the study of inflammation of the blood-brain barrier.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>7911476</pmid><doi>10.1016/0165-5728(94)90155-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of neuroimmunology, 1994-06, Vol.52 (1), p.1-8 |
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| subjects | Animals Antigens, CD - physiology Biological and medical sciences Blood-Brain Barrier Brain - blood supply Capillaries - cytology Cattle CD18 Antigens Cell Adhesion Cell Adhesion Molecules - physiology Cells, Cultured Cerebral endothelial cells Endothelium, Vascular - cytology Endothelium, Vascular - physiology Fundamental and applied biological sciences. Psychology Inflammation Intercellular Adhesion Molecule-1 Interleukin Lipopolysaccharide Lipopolysaccharides - pharmacology Lymphocyte Function-Associated Antigen-1 - physiology Lymphocytes - physiology Molecular and cellular biology Receptors, Very Late Antigen - physiology |
| title | Lymphocyte adhesion to brain capillary endothelial cells in vitro |
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