An Improved Method for the Purification of Human Erythropoietin with High in Vivo Activity from the Urine of Anemic Patients

An improved method for the purification of human erythropoietin with high in vivo activity from urine was developed. This method involved ion-exchange, gel permeation, affinity chromatography, and reverse-phase chromatography but did not involve any stabilizing procedures. The purified human urinary...

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Veröffentlicht in:	Biological & pharmaceutical bulletin 1994/02/15, Vol.17(2), pp.180-184
Hauptverfasser:	INOUE, Noboru, WADA, Michihito, TAKEUCHI, Makoto
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Anemia - urine Animals Antibodies, Monoclonal Biological and medical sciences CHO Cells Chromatography, Affinity Chromatography, Gel Chromatography, High Pressure Liquid Chromatography, Ion Exchange Cricetinae Electrophoresis, Polyacrylamide Gel erythropoietin Erythropoietin - chemistry Erythropoietin - isolation & purification Erythropoietin - pharmacology Erythropoietin - urine Ethanol General pharmacology Humans Medical sciences molecular size Molecular Weight Pharmacology. Drug treatments Phenol Phenols Recombinant Proteins - chemistry Recombinant Proteins - pharmacology sugar chain
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