Synchronization of Plasmodium yoelii nigeriensis and P. y. killicki infection in the mouse by means of Percoll-glucose gradient stage fractionation: determination of the duration of the schizogonic cycle
The youngest (rings and young trophozoites) erythrocytic stages of Plasmodium yoelii nigeriensis and P. yoelii killicki, two rodent malaria subspecies developing very asynchronously in the blood, were separated from the other stages using a discontinuous Percoll-glucose gradient. They were inoculate...
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Veröffentlicht in: | Parasitology research (1987) 1994-02, Vol.80 (2), p.159-164 |
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creator | DEHARO, E GAUTRET, P GINSBURG, H CHABAUD, A. G LANDAU, I |
description | The youngest (rings and young trophozoites) erythrocytic stages of Plasmodium yoelii nigeriensis and P. yoelii killicki, two rodent malaria subspecies developing very asynchronously in the blood, were separated from the other stages using a discontinuous Percoll-glucose gradient. They were inoculated into mice and the subsequent infection remained synchronous for two generations. The duration of the asexual cycle was found to be 18 h. |
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The duration of the asexual cycle was found to be 18 h.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/BF00933785</identifier><identifier>PMID: 8202457</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Biological and medical sciences ; Centrifugation, Density Gradient - methods ; Erythrocytes - parasitology ; Fundamental and applied biological sciences. Psychology ; Glucose ; Invertebrates ; Life cycle. Host-agent relationship. 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G</creatorcontrib><creatorcontrib>LANDAU, I</creatorcontrib><title>Synchronization of Plasmodium yoelii nigeriensis and P. y. killicki infection in the mouse by means of Percoll-glucose gradient stage fractionation: determination of the duration of the schizogonic cycle</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><description>The youngest (rings and young trophozoites) erythrocytic stages of Plasmodium yoelii nigeriensis and P. yoelii killicki, two rodent malaria subspecies developing very asynchronously in the blood, were separated from the other stages using a discontinuous Percoll-glucose gradient. They were inoculated into mice and the subsequent infection remained synchronous for two generations. The duration of the asexual cycle was found to be 18 h.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Centrifugation, Density Gradient - methods</subject><subject>Erythrocytes - parasitology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose</subject><subject>Invertebrates</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Malaria - parasitology</subject><subject>Malaria - physiopathology</subject><subject>Mice</subject><subject>Plasmodium yoelii - growth & development</subject><subject>Plasmodium yoelii - isolation & purification</subject><subject>Plasmodium yoelii - parasitology</subject><subject>Povidone</subject><subject>Protozoa</subject><subject>Silicon Dioxide</subject><subject>Species Specificity</subject><subject>Time Factors</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEFv1DAQhS0EKkvhwh3JB8Qtix3HTsINKgpIlVqp5bxy7HF2qGMvdnJI_2L_FGZZVeJk2e_5mzePkLecbTlj7ccvl4z1QrSdfEY2vBF1xXspn5NNea0rxrl4SV7l_Isx3qqmOSNnXc3qRrYb8ni7BrNPMeCDnjEGGh298TpP0eIy0TWCR6QBR0gIIWOmOlh6s6Xrlt6j92jukWJwYI6_MdB5D3SKSwY6rHQCHfKRCclE76vRLyYWbUzaFuBM86xHoC7pI-CY4RO1MEOaMDxF-gu1S_rvns0eH-JYohtqVuPhNXnhtM_w5nSek5-XX-8uvldX199-XHy-qg61kHOlhtY2vdXSKFCiU41zXFmlWKdd54BpKQelHeN9DU3dic4q6XTLhOUM-oGLc_LhH_eQ4u8F8rybMBvwXgcoi-9aJcuguinGdyfjMkxgd4eEk07r7tR-0d-fdJ2N9qWEYDA_2UQvm1Zw8Qf5Mpeg</recordid><startdate>19940201</startdate><enddate>19940201</enddate><creator>DEHARO, E</creator><creator>GAUTRET, P</creator><creator>GINSBURG, H</creator><creator>CHABAUD, A. 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Pathogenesis</topic><topic>Malaria - parasitology</topic><topic>Malaria - physiopathology</topic><topic>Mice</topic><topic>Plasmodium yoelii - growth & development</topic><topic>Plasmodium yoelii - isolation & purification</topic><topic>Plasmodium yoelii - parasitology</topic><topic>Povidone</topic><topic>Protozoa</topic><topic>Silicon Dioxide</topic><topic>Species Specificity</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DEHARO, E</creatorcontrib><creatorcontrib>GAUTRET, P</creatorcontrib><creatorcontrib>GINSBURG, H</creatorcontrib><creatorcontrib>CHABAUD, A. G</creatorcontrib><creatorcontrib>LANDAU, I</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DEHARO, E</au><au>GAUTRET, P</au><au>GINSBURG, H</au><au>CHABAUD, A. G</au><au>LANDAU, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synchronization of Plasmodium yoelii nigeriensis and P. y. killicki infection in the mouse by means of Percoll-glucose gradient stage fractionation: determination of the duration of the schizogonic cycle</atitle><jtitle>Parasitology research (1987)</jtitle><addtitle>Parasitol Res</addtitle><date>1994-02-01</date><risdate>1994</risdate><volume>80</volume><issue>2</issue><spage>159</spage><epage>164</epage><pages>159-164</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>The youngest (rings and young trophozoites) erythrocytic stages of Plasmodium yoelii nigeriensis and P. yoelii killicki, two rodent malaria subspecies developing very asynchronously in the blood, were separated from the other stages using a discontinuous Percoll-glucose gradient. They were inoculated into mice and the subsequent infection remained synchronous for two generations. The duration of the asexual cycle was found to be 18 h.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>8202457</pmid><doi>10.1007/BF00933785</doi><tpages>6</tpages></addata></record> |
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source | MEDLINE; Springer Nature - Complete Springer Journals |
subjects | Animals Biological and medical sciences Centrifugation, Density Gradient - methods Erythrocytes - parasitology Fundamental and applied biological sciences. Psychology Glucose Invertebrates Life cycle. Host-agent relationship. Pathogenesis Malaria - parasitology Malaria - physiopathology Mice Plasmodium yoelii - growth & development Plasmodium yoelii - isolation & purification Plasmodium yoelii - parasitology Povidone Protozoa Silicon Dioxide Species Specificity Time Factors |
title | Synchronization of Plasmodium yoelii nigeriensis and P. y. killicki infection in the mouse by means of Percoll-glucose gradient stage fractionation: determination of the duration of the schizogonic cycle |
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