Synchronization of Plasmodium yoelii nigeriensis and P. y. killicki infection in the mouse by means of Percoll-glucose gradient stage fractionation: determination of the duration of the schizogonic cycle

The youngest (rings and young trophozoites) erythrocytic stages of Plasmodium yoelii nigeriensis and P. yoelii killicki, two rodent malaria subspecies developing very asynchronously in the blood, were separated from the other stages using a discontinuous Percoll-glucose gradient. They were inoculate...

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Veröffentlicht in:Parasitology research (1987) 1994-02, Vol.80 (2), p.159-164
Hauptverfasser: DEHARO, E, GAUTRET, P, GINSBURG, H, CHABAUD, A. G, LANDAU, I
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container_title Parasitology research (1987)
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creator DEHARO, E
GAUTRET, P
GINSBURG, H
CHABAUD, A. G
LANDAU, I
description The youngest (rings and young trophozoites) erythrocytic stages of Plasmodium yoelii nigeriensis and P. yoelii killicki, two rodent malaria subspecies developing very asynchronously in the blood, were separated from the other stages using a discontinuous Percoll-glucose gradient. They were inoculated into mice and the subsequent infection remained synchronous for two generations. The duration of the asexual cycle was found to be 18 h.
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source MEDLINE; Springer Nature - Complete Springer Journals
subjects Animals
Biological and medical sciences
Centrifugation, Density Gradient - methods
Erythrocytes - parasitology
Fundamental and applied biological sciences. Psychology
Glucose
Invertebrates
Life cycle. Host-agent relationship. Pathogenesis
Malaria - parasitology
Malaria - physiopathology
Mice
Plasmodium yoelii - growth & development
Plasmodium yoelii - isolation & purification
Plasmodium yoelii - parasitology
Povidone
Protozoa
Silicon Dioxide
Species Specificity
Time Factors
title Synchronization of Plasmodium yoelii nigeriensis and P. y. killicki infection in the mouse by means of Percoll-glucose gradient stage fractionation: determination of the duration of the schizogonic cycle
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