Prostaglandin biosynthesis by fat body from the tobacco hornworm, Manduca sexta
We describe prostaglandin (PG) biosynthesis by microsomal-enriched preparations of fat body from larvae of the tobacco hornworm Manduca sexta. Four major PGs were synthesized under most experimental conditions, PGA 2, PGE 2, PGD 2 and PGF 2α. PGA 2, was the predominant product under most conditions....
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| Veröffentlicht in: | Insect biochemistry and molecular biology 1994-05, Vol.24 (5), p.481-491 |
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| creator | Stanley-Samuelson, David W. Ogg, Clyde L. |
| description | We describe prostaglandin (PG) biosynthesis by microsomal-enriched preparations of fat body from larvae of the tobacco hornworm
Manduca sexta. Four major PGs were synthesized under most experimental conditions, PGA
2, PGE
2, PGD
2 and PGF
2α. PGA
2, was the predominant product under most conditions. Unlike mammals, in which PGA
2, is generally thought to arise from non-enzymatic rearrangemets of PGE
2, the fat body preparations did not convert exogenous PGE
2 into PGA
2. These findings suggest that PGA
2 is an important fat body product that is synthesized by a route that does not involve PGE
2. The PG synthase activity and the overall profile of PG synthesis were sensitive to experimental conditions, including incubation time, temperature, and protein concentration. Optimal PG biosynthesis was observed with 1 mg of microsomal-rich protein, incubated at 30°C for 1–2 min. The fat body preparation is sensitive to two non-steroidal anti-inflammatory drugs, indomethacin and naproxen, both of which inhibited PG synthesis at low dosages. |
| doi_str_mv | 10.1016/0965-1748(94)90043-4 |
| format | Article |
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Manduca sexta. Four major PGs were synthesized under most experimental conditions, PGA
2, PGE
2, PGD
2 and PGF
2α. PGA
2, was the predominant product under most conditions. Unlike mammals, in which PGA
2, is generally thought to arise from non-enzymatic rearrangemets of PGE
2, the fat body preparations did not convert exogenous PGE
2 into PGA
2. These findings suggest that PGA
2 is an important fat body product that is synthesized by a route that does not involve PGE
2. The PG synthase activity and the overall profile of PG synthesis were sensitive to experimental conditions, including incubation time, temperature, and protein concentration. Optimal PG biosynthesis was observed with 1 mg of microsomal-rich protein, incubated at 30°C for 1–2 min. The fat body preparation is sensitive to two non-steroidal anti-inflammatory drugs, indomethacin and naproxen, both of which inhibited PG synthesis at low dosages.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/0965-1748(94)90043-4</identifier><identifier>PMID: 8205144</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Arachidonic Acid - metabolism ; Biochemistry. Physiology. Immunology ; Biological and medical sciences ; Fat Body - metabolism ; Fundamental and applied biological sciences. Psychology ; Hydroxyeicosatetraenoic Acids - metabolism ; Indomethacin - pharmacology ; Insect immunity ; Insecta ; Invertebrates ; Larva - metabolism ; Lepidoptera ; Manduca sexta ; Microsomes - metabolism ; Moths - metabolism ; Naproxen - pharmacology ; Physiology. Development ; Prostaglandin ; Prostaglandin-Endoperoxide Synthases - metabolism ; Prostaglandins - biosynthesis ; Prostaglandins A - biosynthesis ; Sphingidae ; Temperature</subject><ispartof>Insect biochemistry and molecular biology, 1994-05, Vol.24 (5), p.481-491</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-93374ab950407635b7dbdfad38b8c7221c2b0b402dcb29d108280f255bdc37da3</citedby><cites>FETCH-LOGICAL-c417t-93374ab950407635b7dbdfad38b8c7221c2b0b402dcb29d108280f255bdc37da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0965-1748(94)90043-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4269623$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8205144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stanley-Samuelson, David W.</creatorcontrib><creatorcontrib>Ogg, Clyde L.</creatorcontrib><title>Prostaglandin biosynthesis by fat body from the tobacco hornworm, Manduca sexta</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>We describe prostaglandin (PG) biosynthesis by microsomal-enriched preparations of fat body from larvae of the tobacco hornworm
Manduca sexta. Four major PGs were synthesized under most experimental conditions, PGA
2, PGE
2, PGD
2 and PGF
2α. PGA
2, was the predominant product under most conditions. Unlike mammals, in which PGA
2, is generally thought to arise from non-enzymatic rearrangemets of PGE
2, the fat body preparations did not convert exogenous PGE
2 into PGA
2. These findings suggest that PGA
2 is an important fat body product that is synthesized by a route that does not involve PGE
2. The PG synthase activity and the overall profile of PG synthesis were sensitive to experimental conditions, including incubation time, temperature, and protein concentration. Optimal PG biosynthesis was observed with 1 mg of microsomal-rich protein, incubated at 30°C for 1–2 min. The fat body preparation is sensitive to two non-steroidal anti-inflammatory drugs, indomethacin and naproxen, both of which inhibited PG synthesis at low dosages.</description><subject>Animals</subject><subject>Arachidonic Acid - metabolism</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>Fat Body - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydroxyeicosatetraenoic Acids - metabolism</subject><subject>Indomethacin - pharmacology</subject><subject>Insect immunity</subject><subject>Insecta</subject><subject>Invertebrates</subject><subject>Larva - metabolism</subject><subject>Lepidoptera</subject><subject>Manduca sexta</subject><subject>Microsomes - metabolism</subject><subject>Moths - metabolism</subject><subject>Naproxen - pharmacology</subject><subject>Physiology. Development</subject><subject>Prostaglandin</subject><subject>Prostaglandin-Endoperoxide Synthases - metabolism</subject><subject>Prostaglandins - biosynthesis</subject><subject>Prostaglandins A - biosynthesis</subject><subject>Sphingidae</subject><subject>Temperature</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1LHDEUhkNRdNX-gwq5EKngtPk4k0xuBBG1gqIX7XXI19SUnYlNZmv33zfbXfZSr3LIed7DOQ9Cnyj5QgkVX4kSbUMldJ8VnClCgDfwAc1oJ1VDGJAdNNsi--iglF-kQtDKPbTXMdJSgBl6fMqpTObn3Iw-jtjGVJbj9BxKLNgucW8mbJOvRU4Drv94StY4l_BzyuNrysM5fqjRhTO4hL-TOUK7vZmX8HHzHqIfN9ffr74194-3d1eX940DKqdGcS7BWNUSIFLw1kpvfW8872znJGPUMUssEOadZcpT0rGO9KxtrXdcesMP0el67ktOvxehTHqIxYV5vSOkRdFStFSqTrwLUiFBAKcVhDXoqpGSQ69fchxMXmpK9Eq4XtnUK5tagf4vXEONHW_mL-wQ_Da0MVz7J5u-Kc7M-2xGF8sWAyaUYLxiF2ssVGl_Ysi6uBhGF3zMwU3ap_j2Hv8AYyqbxg</recordid><startdate>19940501</startdate><enddate>19940501</enddate><creator>Stanley-Samuelson, David W.</creator><creator>Ogg, Clyde L.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>19940501</creationdate><title>Prostaglandin biosynthesis by fat body from the tobacco hornworm, Manduca sexta</title><author>Stanley-Samuelson, David W. ; Ogg, Clyde L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-93374ab950407635b7dbdfad38b8c7221c2b0b402dcb29d108280f255bdc37da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Arachidonic Acid - metabolism</topic><topic>Biochemistry. Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>Fat Body - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydroxyeicosatetraenoic Acids - metabolism</topic><topic>Indomethacin - pharmacology</topic><topic>Insect immunity</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>Larva - metabolism</topic><topic>Lepidoptera</topic><topic>Manduca sexta</topic><topic>Microsomes - metabolism</topic><topic>Moths - metabolism</topic><topic>Naproxen - pharmacology</topic><topic>Physiology. Development</topic><topic>Prostaglandin</topic><topic>Prostaglandin-Endoperoxide Synthases - metabolism</topic><topic>Prostaglandins - biosynthesis</topic><topic>Prostaglandins A - biosynthesis</topic><topic>Sphingidae</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stanley-Samuelson, David W.</creatorcontrib><creatorcontrib>Ogg, Clyde L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stanley-Samuelson, David W.</au><au>Ogg, Clyde L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prostaglandin biosynthesis by fat body from the tobacco hornworm, Manduca sexta</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>1994-05-01</date><risdate>1994</risdate><volume>24</volume><issue>5</issue><spage>481</spage><epage>491</epage><pages>481-491</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>We describe prostaglandin (PG) biosynthesis by microsomal-enriched preparations of fat body from larvae of the tobacco hornworm
Manduca sexta. Four major PGs were synthesized under most experimental conditions, PGA
2, PGE
2, PGD
2 and PGF
2α. PGA
2, was the predominant product under most conditions. Unlike mammals, in which PGA
2, is generally thought to arise from non-enzymatic rearrangemets of PGE
2, the fat body preparations did not convert exogenous PGE
2 into PGA
2. These findings suggest that PGA
2 is an important fat body product that is synthesized by a route that does not involve PGE
2. The PG synthase activity and the overall profile of PG synthesis were sensitive to experimental conditions, including incubation time, temperature, and protein concentration. Optimal PG biosynthesis was observed with 1 mg of microsomal-rich protein, incubated at 30°C for 1–2 min. The fat body preparation is sensitive to two non-steroidal anti-inflammatory drugs, indomethacin and naproxen, both of which inhibited PG synthesis at low dosages.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8205144</pmid><doi>10.1016/0965-1748(94)90043-4</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Insect biochemistry and molecular biology, 1994-05, Vol.24 (5), p.481-491 |
| issn | 0965-1748 1879-0240 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76517986 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Arachidonic Acid - metabolism Biochemistry. Physiology. Immunology Biological and medical sciences Fat Body - metabolism Fundamental and applied biological sciences. Psychology Hydroxyeicosatetraenoic Acids - metabolism Indomethacin - pharmacology Insect immunity Insecta Invertebrates Larva - metabolism Lepidoptera Manduca sexta Microsomes - metabolism Moths - metabolism Naproxen - pharmacology Physiology. Development Prostaglandin Prostaglandin-Endoperoxide Synthases - metabolism Prostaglandins - biosynthesis Prostaglandins A - biosynthesis Sphingidae Temperature |
| title | Prostaglandin biosynthesis by fat body from the tobacco hornworm, Manduca sexta |
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