Heterogeneity of human natural killer recognition demonstrated by cloned effector cells and differential blocking of cytotoxicity with monoclonal antibodies
Monoclonal antibodies (MoAb) against cell surface determinants were employed to investigate the specificity of natural killer (NK)-like lysis by cloned human effector cells recognizing only K562, only HSB2, or both K562 and HSB2 target cells. MoAb W6/32.HL, TÜ39, YD1/48.HLK, and anti-Tac failed to i...
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| Veröffentlicht in: | Cellular immunology 1985-04, Vol.92 (1), p.31-40 |
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| creator | Pawelec, Graham Newman, Walter Schwulera, Udo Wernet, Peter |
| description | Monoclonal antibodies (MoAb) against cell surface determinants were employed to investigate the specificity of natural killer (NK)-like lysis by cloned human effector cells recognizing only K562, only HSB2, or both K562 and HSB2 target cells. MoAb W6/32.HL, TÜ39, YD1/48.HLK, and anti-Tac failed to inhibit lysis despite the expression of antigens bound by these MoAb on the effector cell surface. MoAb OKT3 moderately (⪕50%) blocked lysis of K562 and HSB2 targets, whereas MoAb 13.1, which binds T200 molecules, strongly (up to 95%) blocked lysis of K562, but not HSB2, targets. MoAb 13.1 inhibited lysis by clones which killed only K562, as well as lysis by those which killed both HSB2 and K562. In the latter case, however, only lysis of K562 was inhibited. Taken together, these results may suggest the existence of multiple receptor specificities on a single NK-active clone. |
| doi_str_mv | 10.1016/0008-8749(85)90062-0 |
| format | Article |
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MoAb W6/32.HL, TÜ39, YD1/48.HLK, and anti-Tac failed to inhibit lysis despite the expression of antigens bound by these MoAb on the effector cell surface. MoAb OKT3 moderately (⪕50%) blocked lysis of K562 and HSB2 targets, whereas MoAb 13.1, which binds T200 molecules, strongly (up to 95%) blocked lysis of K562, but not HSB2, targets. MoAb 13.1 inhibited lysis by clones which killed only K562, as well as lysis by those which killed both HSB2 and K562. In the latter case, however, only lysis of K562 was inhibited. 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MoAb W6/32.HL, TÜ39, YD1/48.HLK, and anti-Tac failed to inhibit lysis despite the expression of antigens bound by these MoAb on the effector cell surface. MoAb OKT3 moderately (⪕50%) blocked lysis of K562 and HSB2 targets, whereas MoAb 13.1, which binds T200 molecules, strongly (up to 95%) blocked lysis of K562, but not HSB2, targets. MoAb 13.1 inhibited lysis by clones which killed only K562, as well as lysis by those which killed both HSB2 and K562. In the latter case, however, only lysis of K562 was inhibited. Taken together, these results may suggest the existence of multiple receptor specificities on a single NK-active clone.</description><subject>Antibodies, Monoclonal - physiology</subject><subject>Antibody Specificity</subject><subject>Antigens, Surface - analysis</subject><subject>Applied sciences</subject><subject>Binding, Competitive</subject><subject>Cell Line</subject><subject>Clone Cells - classification</subject><subject>Clone Cells - immunology</subject><subject>Cytotoxicity, Immunologic</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Killer Cells, Natural - classification</subject><subject>Killer Cells, Natural - immunology</subject><subject>Leukemia, Erythroblastic, Acute - immunology</subject><subject>Leukemia, Myeloid - immunology</subject><subject>Other techniques and industries</subject><issn>0008-8749</issn><issn>1090-2163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-OFCEQhztGs86uvoEmHIzRQ2vRdNP0xcRs1DXZxIueCX-KWdweWIFenXfxYaWdyRz1BKE-fgX1Nc0zCm8oUP4WAEQrxn56JYbXEwDvWnjQbChM0HaUs4fN5oQ8bs5z_g5AaT_BWXPGBB9GyjfN7yssmOIWA_qyJ9GRm2WnAgmqLEnN5NbPMyaS0MRt8MXHQCzuYsglqYKW6D0xcwx1h86hKTERg_OciQqWWF_PEobia5Keo7n1Ybv2MPsSS_zlzdrzpy83pEbGNaiCqvI6Wo_5SfPIqTnj0-N60Xz7-OHr5VV7_eXT58v3161hrCstBwZUDxQHzpxWrKO9GGwvNFfTRMEKGPnYo-HARzPAqJBi5zR1I9VWu45dNC8PuXcp_lgwF7nzef2GChiXLEc-0JH27L8g7Wkd6zBVsD-AJsWcEzp5l_xOpb2kIFd7clUjVzVSDPKvPQn12vNj_qJ3aE-Xjrpq_cWxrrJRs0sqGJ9PmBBsFKKv2LsDhnVo9x6TzMZjMGh9FVmkjf7f7_gDddq5wA</recordid><startdate>19850415</startdate><enddate>19850415</enddate><creator>Pawelec, Graham</creator><creator>Newman, Walter</creator><creator>Schwulera, Udo</creator><creator>Wernet, Peter</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19850415</creationdate><title>Heterogeneity of human natural killer recognition demonstrated by cloned effector cells and differential blocking of cytotoxicity with monoclonal antibodies</title><author>Pawelec, Graham ; Newman, Walter ; Schwulera, Udo ; Wernet, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-60301b51e563fba321485d48b6a9910d807674ec6067c507ae1e2fb1f71bdbf23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Antibodies, Monoclonal - physiology</topic><topic>Antibody Specificity</topic><topic>Antigens, Surface - analysis</topic><topic>Applied sciences</topic><topic>Binding, Competitive</topic><topic>Cell Line</topic><topic>Clone Cells - classification</topic><topic>Clone Cells - immunology</topic><topic>Cytotoxicity, Immunologic</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Killer Cells, Natural - classification</topic><topic>Killer Cells, Natural - immunology</topic><topic>Leukemia, Erythroblastic, Acute - immunology</topic><topic>Leukemia, Myeloid - immunology</topic><topic>Other techniques and industries</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pawelec, Graham</creatorcontrib><creatorcontrib>Newman, Walter</creatorcontrib><creatorcontrib>Schwulera, Udo</creatorcontrib><creatorcontrib>Wernet, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pawelec, Graham</au><au>Newman, Walter</au><au>Schwulera, Udo</au><au>Wernet, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterogeneity of human natural killer recognition demonstrated by cloned effector cells and differential blocking of cytotoxicity with monoclonal antibodies</atitle><jtitle>Cellular immunology</jtitle><addtitle>Cell Immunol</addtitle><date>1985-04-15</date><risdate>1985</risdate><volume>92</volume><issue>1</issue><spage>31</spage><epage>40</epage><pages>31-40</pages><issn>0008-8749</issn><eissn>1090-2163</eissn><coden>CLIMB8</coden><abstract>Monoclonal antibodies (MoAb) against cell surface determinants were employed to investigate the specificity of natural killer (NK)-like lysis by cloned human effector cells recognizing only K562, only HSB2, or both K562 and HSB2 target cells. MoAb W6/32.HL, TÜ39, YD1/48.HLK, and anti-Tac failed to inhibit lysis despite the expression of antigens bound by these MoAb on the effector cell surface. MoAb OKT3 moderately (⪕50%) blocked lysis of K562 and HSB2 targets, whereas MoAb 13.1, which binds T200 molecules, strongly (up to 95%) blocked lysis of K562, but not HSB2, targets. MoAb 13.1 inhibited lysis by clones which killed only K562, as well as lysis by those which killed both HSB2 and K562. In the latter case, however, only lysis of K562 was inhibited. Taken together, these results may suggest the existence of multiple receptor specificities on a single NK-active clone.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3865716</pmid><doi>10.1016/0008-8749(85)90062-0</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-8749 |
| ispartof | Cellular immunology, 1985-04, Vol.92 (1), p.31-40 |
| issn | 0008-8749 1090-2163 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antibodies, Monoclonal - physiology Antibody Specificity Antigens, Surface - analysis Applied sciences Binding, Competitive Cell Line Clone Cells - classification Clone Cells - immunology Cytotoxicity, Immunologic Exact sciences and technology Humans Killer Cells, Natural - classification Killer Cells, Natural - immunology Leukemia, Erythroblastic, Acute - immunology Leukemia, Myeloid - immunology Other techniques and industries |
| title | Heterogeneity of human natural killer recognition demonstrated by cloned effector cells and differential blocking of cytotoxicity with monoclonal antibodies |
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