Bioluminescent enzyme immunoassay for estriol. Use of reversibly inactivated bacterial luciferase as label

A bioluminescent enzyme immunoassay using estriol labeled with reversibly inactivated bacterial luciferase is described. An estriol derivative bearing an alkylthiolsulfonate is linked to the cysteinyl thiols of luciferase by formation of mixed disulfide linkages; thus, luciferase becomes inactive. A...

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Veröffentlicht in:	Analytical biochemistry 1985-09, Vol.149 (2), p.309-315
Hauptverfasser:	Yein, Fredrick S., Marschke, Charles K., Deming, Philip C., Holzman, Thomas F., Satoh, Paul S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Applied sciences Biological and medical sciences enzyme immunoassay Estradiol - analysis estriol Estriol - analogs & derivatives Exact sciences and technology Fundamental and applied biological sciences. Psychology Immunoenzyme Techniques Luciferases - antagonists & inhibitors luminescence Luminescent Measurements Other biological molecules Other techniques and industries reversibly inhibited luciferase Terpenes, steroids. Hormones Vibrio - enzymology
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