Bioluminescent enzyme immunoassay for estriol. Use of reversibly inactivated bacterial luciferase as label
A bioluminescent enzyme immunoassay using estriol labeled with reversibly inactivated bacterial luciferase is described. An estriol derivative bearing an alkylthiolsulfonate is linked to the cysteinyl thiols of luciferase by formation of mixed disulfide linkages; thus, luciferase becomes inactive. A...
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| Veröffentlicht in: | Analytical biochemistry 1985-09, Vol.149 (2), p.309-315 |
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| container_issue | 2 |
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| container_title | Analytical biochemistry |
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| creator | Yein, Fredrick S. Marschke, Charles K. Deming, Philip C. Holzman, Thomas F. Satoh, Paul S. |
| description | A bioluminescent enzyme immunoassay using estriol labeled with reversibly inactivated bacterial luciferase is described. An estriol derivative bearing an alkylthiolsulfonate is linked to the cysteinyl thiols of luciferase by formation of mixed disulfide linkages; thus, luciferase becomes inactive. After immunoassay, the inactive luciferase of the label bound to the immunoprecipitate is reactivated by incubation with dithiothreitol and the luciferase activity then is quantitated by a 20-s reaction performed with an automated luminometer (LKB 1251). Under the defined conditions, the labels are stable for at least 14 days as tested at 4°C. A standard curve with a wide linear range from 50 to 6000 pg is demonstrated. This unique technology discussed here, therefore, offers exciting possibilities as a sensitive and rapid enzyme immunoassay for estriol. |
| doi_str_mv | 10.1016/0003-2697(85)90575-5 |
| format | Article |
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Use of reversibly inactivated bacterial luciferase as label</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Yein, Fredrick S. ; Marschke, Charles K. ; Deming, Philip C. ; Holzman, Thomas F. ; Satoh, Paul S.</creator><creatorcontrib>Yein, Fredrick S. ; Marschke, Charles K. ; Deming, Philip C. ; Holzman, Thomas F. ; Satoh, Paul S.</creatorcontrib><description>A bioluminescent enzyme immunoassay using estriol labeled with reversibly inactivated bacterial luciferase is described. An estriol derivative bearing an alkylthiolsulfonate is linked to the cysteinyl thiols of luciferase by formation of mixed disulfide linkages; thus, luciferase becomes inactive. After immunoassay, the inactive luciferase of the label bound to the immunoprecipitate is reactivated by incubation with dithiothreitol and the luciferase activity then is quantitated by a 20-s reaction performed with an automated luminometer (LKB 1251). Under the defined conditions, the labels are stable for at least 14 days as tested at 4°C. A standard curve with a wide linear range from 50 to 6000 pg is demonstrated. This unique technology discussed here, therefore, offers exciting possibilities as a sensitive and rapid enzyme immunoassay for estriol.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(85)90575-5</identifier><identifier>PMID: 3907406</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Analytical, structural and metabolic biochemistry ; Applied sciences ; Biological and medical sciences ; enzyme immunoassay ; Estradiol - analysis ; estriol ; Estriol - analogs & derivatives ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Immunoenzyme Techniques ; Luciferases - antagonists & inhibitors ; luminescence ; Luminescent Measurements ; Other biological molecules ; Other techniques and industries ; reversibly inhibited luciferase ; Terpenes, steroids. 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Use of reversibly inactivated bacterial luciferase as label</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A bioluminescent enzyme immunoassay using estriol labeled with reversibly inactivated bacterial luciferase is described. An estriol derivative bearing an alkylthiolsulfonate is linked to the cysteinyl thiols of luciferase by formation of mixed disulfide linkages; thus, luciferase becomes inactive. After immunoassay, the inactive luciferase of the label bound to the immunoprecipitate is reactivated by incubation with dithiothreitol and the luciferase activity then is quantitated by a 20-s reaction performed with an automated luminometer (LKB 1251). Under the defined conditions, the labels are stable for at least 14 days as tested at 4°C. A standard curve with a wide linear range from 50 to 6000 pg is demonstrated. This unique technology discussed here, therefore, offers exciting possibilities as a sensitive and rapid enzyme immunoassay for estriol.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>enzyme immunoassay</subject><subject>Estradiol - analysis</subject><subject>estriol</subject><subject>Estriol - analogs & derivatives</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoenzyme Techniques</subject><subject>Luciferases - antagonists & inhibitors</subject><subject>luminescence</subject><subject>Luminescent Measurements</subject><subject>Other biological molecules</subject><subject>Other techniques and industries</subject><subject>reversibly inhibited luciferase</subject><subject>Terpenes, steroids. Hormones</subject><subject>Vibrio - enzymology</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFq3DAQhkVoSTZp3iABHUJpD05HtiRbl0Ib0rQQyCU5C1kagYJsJ5K9sHn6arvLHtODGMF8M_zzEXLB4JoBk98AoKlqqdovnfiqQLSiEkdkxUDJChpQH8jqgJyQ05yfARjjQh6T40ZBy0GuyPPPMMVlCCNmi-NMcXzbDEjDMCzjZHI2G-qnRDHPqYDX9CkjnTxNuMaUQx83NIzGzmFtZnS0L19MwUQaFxs8JlNwk2k0PcZP5KM3MeP5vp6Rp1-3jze_q_uHuz83P-4r20g-V9yK1svyOux81ztb1yidLXmFsTUH43opDXfInGNW9crVSvXeKi-62nlszsjn3d6XNL0uJbkeQjkuRjPitGTdSsGYBF5AvgNtmnJO6PVLCoNJG81AbxXrrT-99ac7of8p1qKMXe73L_2A7jC0d1r6V_u-ydZEn8xoQz5gXQeNavj_sFaVg-vttu87DIuydcCksw04WnQhoZ21m8L7cf8CV2mnKg</recordid><startdate>198509</startdate><enddate>198509</enddate><creator>Yein, Fredrick S.</creator><creator>Marschke, Charles K.</creator><creator>Deming, Philip C.</creator><creator>Holzman, Thomas F.</creator><creator>Satoh, Paul S.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198509</creationdate><title>Bioluminescent enzyme immunoassay for estriol. Use of reversibly inactivated bacterial luciferase as label</title><author>Yein, Fredrick S. ; Marschke, Charles K. ; Deming, Philip C. ; Holzman, Thomas F. ; Satoh, Paul S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-4c57f657f8e8f8bdc22e6dc0745ac240adb66a4de1dd1c9b9d299bfc9f582dfe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>enzyme immunoassay</topic><topic>Estradiol - analysis</topic><topic>estriol</topic><topic>Estriol - analogs & derivatives</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoenzyme Techniques</topic><topic>Luciferases - antagonists & inhibitors</topic><topic>luminescence</topic><topic>Luminescent Measurements</topic><topic>Other biological molecules</topic><topic>Other techniques and industries</topic><topic>reversibly inhibited luciferase</topic><topic>Terpenes, steroids. Hormones</topic><topic>Vibrio - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yein, Fredrick S.</creatorcontrib><creatorcontrib>Marschke, Charles K.</creatorcontrib><creatorcontrib>Deming, Philip C.</creatorcontrib><creatorcontrib>Holzman, Thomas F.</creatorcontrib><creatorcontrib>Satoh, Paul S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yein, Fredrick S.</au><au>Marschke, Charles K.</au><au>Deming, Philip C.</au><au>Holzman, Thomas F.</au><au>Satoh, Paul S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioluminescent enzyme immunoassay for estriol. Use of reversibly inactivated bacterial luciferase as label</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1985-09</date><risdate>1985</risdate><volume>149</volume><issue>2</issue><spage>309</spage><epage>315</epage><pages>309-315</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>A bioluminescent enzyme immunoassay using estriol labeled with reversibly inactivated bacterial luciferase is described. An estriol derivative bearing an alkylthiolsulfonate is linked to the cysteinyl thiols of luciferase by formation of mixed disulfide linkages; thus, luciferase becomes inactive. After immunoassay, the inactive luciferase of the label bound to the immunoprecipitate is reactivated by incubation with dithiothreitol and the luciferase activity then is quantitated by a 20-s reaction performed with an automated luminometer (LKB 1251). Under the defined conditions, the labels are stable for at least 14 days as tested at 4°C. A standard curve with a wide linear range from 50 to 6000 pg is demonstrated. This unique technology discussed here, therefore, offers exciting possibilities as a sensitive and rapid enzyme immunoassay for estriol.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3907406</pmid><doi>10.1016/0003-2697(85)90575-5</doi><tpages>7</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 1985-09, Vol.149 (2), p.309-315 |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Analytical, structural and metabolic biochemistry Applied sciences Biological and medical sciences enzyme immunoassay Estradiol - analysis estriol Estriol - analogs & derivatives Exact sciences and technology Fundamental and applied biological sciences. Psychology Immunoenzyme Techniques Luciferases - antagonists & inhibitors luminescence Luminescent Measurements Other biological molecules Other techniques and industries reversibly inhibited luciferase Terpenes, steroids. Hormones Vibrio - enzymology |
| title | Bioluminescent enzyme immunoassay for estriol. Use of reversibly inactivated bacterial luciferase as label |
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