Spectrophotometric method of controlled pheophytinization for the determination of both chlorophylls and pheophytins in plant extracts
Conventional spectrophotometric methods of chlorophyll (Chl) measurement with double-wavelength readings of absorbances corresponding to the peak values of both Chl a and Chl b are usable only is pheophytins (Pheo) are absent in the pigment extract. We present here a kinetic method of controlled phe...
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| Veröffentlicht in: | Analytical biochemistry 1985-08, Vol.149 (1), p.121-129 |
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| container_title | Analytical biochemistry |
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| creator | Laval-Martin, Danielle L. |
| description | Conventional spectrophotometric methods of chlorophyll (Chl) measurement with double-wavelength readings of absorbances corresponding to the peak values of both Chl
a and Chl
b are usable only is pheophytins (Pheo) are absent in the pigment extract. We present here a kinetic method of controlled phenophytinization of the Chl present in acetone/water (
90
10
,
v
v
) exctracts that allows the measurement of both Chl
a and
b, as well as of the initial Pheo preexisting in the plant material at the moment of the extraction. This method gives better accuracy in Chl
b determination than conventional methods, particularly when
Chl a
Chl b
ratios are greater than 5. Examples are given illustrating the usefulness of this method. |
| doi_str_mv | 10.1016/0003-2697(85)90484-1 |
| format | Article |
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a and Chl
b are usable only is pheophytins (Pheo) are absent in the pigment extract. We present here a kinetic method of controlled phenophytinization of the Chl present in acetone/water (
90
10
,
v
v
) exctracts that allows the measurement of both Chl
a and
b, as well as of the initial Pheo preexisting in the plant material at the moment of the extraction. This method gives better accuracy in Chl
b determination than conventional methods, particularly when
Chl a
Chl b
ratios are greater than 5. Examples are given illustrating the usefulness of this method.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(85)90484-1</identifier><identifier>PMID: 3935000</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Analytical, structural and metabolic biochemistry ; Applied sciences ; Biological and medical sciences ; chlorophyll ; Chlorophyll - analogs & derivatives ; Chlorophyll - analysis ; Euglena gracilis - analysis ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Heterocyclic compounds, pigments ; Kinetics ; Mathematics ; Other biological molecules ; Other techniques and industries ; pheophytin ; pheophytinization ; Pheophytins - analysis ; Plant Extracts - analysis ; spectrophotometric method ; Spectrophotometry - methods ; Tetrapyrrolic pigments</subject><ispartof>Analytical biochemistry, 1985-08, Vol.149 (1), p.121-129</ispartof><rights>1985</rights><rights>1987 INIST-CNRS</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-dbcf619740da376a78e78f6ace7879d8f325f1663244adbaf37c5b554fd3fdb43</citedby><cites>FETCH-LOGICAL-c481t-dbcf619740da376a78e78f6ace7879d8f325f1663244adbaf37c5b554fd3fdb43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003269785904841$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7974145$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8790190$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3935000$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laval-Martin, Danielle L.</creatorcontrib><title>Spectrophotometric method of controlled pheophytinization for the determination of both chlorophylls and pheophytins in plant extracts</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Conventional spectrophotometric methods of chlorophyll (Chl) measurement with double-wavelength readings of absorbances corresponding to the peak values of both Chl
a and Chl
b are usable only is pheophytins (Pheo) are absent in the pigment extract. We present here a kinetic method of controlled phenophytinization of the Chl present in acetone/water (
90
10
,
v
v
) exctracts that allows the measurement of both Chl
a and
b, as well as of the initial Pheo preexisting in the plant material at the moment of the extraction. This method gives better accuracy in Chl
b determination than conventional methods, particularly when
Chl a
Chl b
ratios are greater than 5. Examples are given illustrating the usefulness of this method.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>chlorophyll</subject><subject>Chlorophyll - analogs & derivatives</subject><subject>Chlorophyll - analysis</subject><subject>Euglena gracilis - analysis</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heterocyclic compounds, pigments</subject><subject>Kinetics</subject><subject>Mathematics</subject><subject>Other biological molecules</subject><subject>Other techniques and industries</subject><subject>pheophytin</subject><subject>pheophytinization</subject><subject>Pheophytins - analysis</subject><subject>Plant Extracts - analysis</subject><subject>spectrophotometric method</subject><subject>Spectrophotometry - methods</subject><subject>Tetrapyrrolic pigments</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2KFDEUhYMoY8_oGyhkITIuSpPOX9VmQIYZFQZcqOuQSm6oSFWlTNKD7QPMc5uym8aVrg7kfPdwcy5CLyh5SwmV7wghrNnKTl224k1HeMsb-ghtKOlkQxjpHqPNCXmKznP-TgilXMgzdMY6Jqq5QQ9fFrAlxWWIJU5QUrC4yhAdjh7bOFdvHMHhZYAK7UuYwy9TQpyxjwmXAbCDAmkK8-G1TvWxDNgOY1xj9-OYsZn_Dsg4zHgZzVww_CzJ2JKfoSfejBmeH_UCfbu9-Xr9sbn7_OHT9fu7xvKWlsb11kvaKU6cYUoa1YJqvTS2iupc69lWeCol23JuXG88U1b0QnDvmHc9Zxfo9SF3SfHHDnLRU8gWxroMxF3WSgpKWkIryA-gTTHnBF4vKUwm7TUleq1fr93qtVvdCv2nfr2OvTzm7_oJ3Gno2Hf1Xx19k60ZfTKzDfmE1U8Q2v0XU7WBesiKXR0wqJXdB0g62wCzBRdSvap2Mfx73d-KabIZ</recordid><startdate>19850815</startdate><enddate>19850815</enddate><creator>Laval-Martin, Danielle L.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19850815</creationdate><title>Spectrophotometric method of controlled pheophytinization for the determination of both chlorophylls and pheophytins in plant extracts</title><author>Laval-Martin, Danielle L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-dbcf619740da376a78e78f6ace7879d8f325f1663244adbaf37c5b554fd3fdb43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>chlorophyll</topic><topic>Chlorophyll - analogs & derivatives</topic><topic>Chlorophyll - analysis</topic><topic>Euglena gracilis - analysis</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heterocyclic compounds, pigments</topic><topic>Kinetics</topic><topic>Mathematics</topic><topic>Other biological molecules</topic><topic>Other techniques and industries</topic><topic>pheophytin</topic><topic>pheophytinization</topic><topic>Pheophytins - analysis</topic><topic>Plant Extracts - analysis</topic><topic>spectrophotometric method</topic><topic>Spectrophotometry - methods</topic><topic>Tetrapyrrolic pigments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laval-Martin, Danielle L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laval-Martin, Danielle L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spectrophotometric method of controlled pheophytinization for the determination of both chlorophylls and pheophytins in plant extracts</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1985-08-15</date><risdate>1985</risdate><volume>149</volume><issue>1</issue><spage>121</spage><epage>129</epage><pages>121-129</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>Conventional spectrophotometric methods of chlorophyll (Chl) measurement with double-wavelength readings of absorbances corresponding to the peak values of both Chl
a and Chl
b are usable only is pheophytins (Pheo) are absent in the pigment extract. We present here a kinetic method of controlled phenophytinization of the Chl present in acetone/water (
90
10
,
v
v
) exctracts that allows the measurement of both Chl
a and
b, as well as of the initial Pheo preexisting in the plant material at the moment of the extraction. This method gives better accuracy in Chl
b determination than conventional methods, particularly when
Chl a
Chl b
ratios are greater than 5. Examples are given illustrating the usefulness of this method.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3935000</pmid><doi>10.1016/0003-2697(85)90484-1</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1985-08, Vol.149 (1), p.121-129 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76510801 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analytical, structural and metabolic biochemistry Applied sciences Biological and medical sciences chlorophyll Chlorophyll - analogs & derivatives Chlorophyll - analysis Euglena gracilis - analysis Exact sciences and technology Fundamental and applied biological sciences. Psychology Heterocyclic compounds, pigments Kinetics Mathematics Other biological molecules Other techniques and industries pheophytin pheophytinization Pheophytins - analysis Plant Extracts - analysis spectrophotometric method Spectrophotometry - methods Tetrapyrrolic pigments |
| title | Spectrophotometric method of controlled pheophytinization for the determination of both chlorophylls and pheophytins in plant extracts |
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