Determination of haptoglobin expression in IL-6 treated HepG2 cells by ELISA and by RNA hybridization — Evaluation of a quantitative method to measure IL-6

Interleukin-6 (IL-6) is known to be an important modulator of acute phase (AP) protein expression in hepatocytes both in vivo and in vitro. In the present study the inducing activity of IL-6 on the expression of the AP protein haptoglobin (HP) by the human hepatoma cell line HepG2, has been evaluate...

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Veröffentlicht in:	Journal of immunological methods 1994-05, Vol.171 (2), p.157-167
Hauptverfasser:	Boe, A., Canosi, U., Donini, S., Mastrangeli, R., Ythier, A., Crescenzi, O.Serlupi
Format:	Artikel
Sprache:	eng
Schlagworte:	Acute phase protein Analytical, structural and metabolic biochemistry Animals Base Sequence Biological and medical sciences Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - metabolism CHO Cells - drug effects Cricetinae Dexamethasone ELISA Enzyme-Linked Immunosorbent Assay Escherichia coli Evaluation Studies as Topic Filter hybridization Fundamental and applied biological sciences. Psychology General aspects, investigation methods Haptoglobins - biosynthesis Haptoglobins - genetics Hepatoma cell Humans IL-6 Interleukin-6 - analysis Interleukin-6 - pharmacology Kinetics Molecular Sequence Data Nucleic Acid Hybridization Proteins RNA, Messenger - biosynthesis RNA, Messenger - genetics Tumor Cells, Cultured - drug effects
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