Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria
Summary Diaminopimelic acid (DAP) is a major component of the peptidoglycan layer of the mycobacterial cell wall. The mycobacterial cell wall has been implicated as a potential virulence factor and is highly immunogenic. The pathway for biosynthesis of DAP may serve as a target in the design of anti...
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| Veröffentlicht in: | Molecular microbiology 1994-02, Vol.11 (4), p.629-639 |
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| creator | Cirillo, Jeffrey D. Weisbrod, Torin R. Pascopella, Lisa Bloom, Barry R. Jacobs, William R. |
| description | Summary
Diaminopimelic acid (DAP) is a major component of the peptidoglycan layer of the mycobacterial cell wall. The mycobacterial cell wall has been implicated as a potential virulence factor and is highly immunogenic. The pathway for biosynthesis of DAP may serve as a target in the design of antimycobacterial agents and construction of in vivo selection systems. Despite its significance, this biosynthetic pathway is poorly understood in mycobacteria. In order to develop a better understanding of mycobacterial DAP biosynthesis, the aspartate semialdehyde dehydrogenase (asd) genes of Mycobacterium smegmatis, bacille Calmette‐Guerin (BCG), Mycobacterium avium, Mycobacterium leprae, and Mycobacterium tuberculosis were isolated. The M. smegmatis asd gene was isolated by complementation in Escherichia coli. This gene was then used to isolate the asd genes from other mycobacteria. The asd‐complemening fragments from BCG and M. smegmatis were sequenced. An open reading frame upstream of the mycobacterial asd gene was identified as the mycobacterial aspartokinase gene (ask). Primer extension analysis revealed that the only transcriptional start in this region is found 5’of the ask gene. This observation indicates that the mycobacterial ask and asd genes are in an operon. |
| doi_str_mv | 10.1111/j.1365-2958.1994.tb00342.x |
| format | Article |
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Diaminopimelic acid (DAP) is a major component of the peptidoglycan layer of the mycobacterial cell wall. The mycobacterial cell wall has been implicated as a potential virulence factor and is highly immunogenic. The pathway for biosynthesis of DAP may serve as a target in the design of antimycobacterial agents and construction of in vivo selection systems. Despite its significance, this biosynthetic pathway is poorly understood in mycobacteria. In order to develop a better understanding of mycobacterial DAP biosynthesis, the aspartate semialdehyde dehydrogenase (asd) genes of Mycobacterium smegmatis, bacille Calmette‐Guerin (BCG), Mycobacterium avium, Mycobacterium leprae, and Mycobacterium tuberculosis were isolated. The M. smegmatis asd gene was isolated by complementation in Escherichia coli. This gene was then used to isolate the asd genes from other mycobacteria. The asd‐complemening fragments from BCG and M. smegmatis were sequenced. An open reading frame upstream of the mycobacterial asd gene was identified as the mycobacterial aspartokinase gene (ask). Primer extension analysis revealed that the only transcriptional start in this region is found 5’of the ask gene. This observation indicates that the mycobacterial ask and asd genes are in an operon.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.1994.tb00342.x</identifier><identifier>PMID: 7910936</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Aspartate Kinase - genetics ; Aspartate-Semialdehyde Dehydrogenase - genetics ; Bacterial Proteins - genetics ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Consensus Sequence ; Diaminopimelic Acid - metabolism ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetic Complementation Test ; Metabolism. Enzymes ; Microbiology ; Molecular Sequence Data ; Mycobacterium - enzymology ; Mycobacterium - genetics ; Mycobacterium avium ; Mycobacterium smegmatis ; Mycobacterium tuberculosis ; Open Reading Frames ; Operon ; Sequence Alignment ; Sequence Homology, Amino Acid</subject><ispartof>Molecular microbiology, 1994-02, Vol.11 (4), p.629-639</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4959-1b83d6dff13638175b5302c2beaac0fc64da07de5cbaeeb7437109a05d98a4c73</citedby><cites>FETCH-LOGICAL-c4959-1b83d6dff13638175b5302c2beaac0fc64da07de5cbaeeb7437109a05d98a4c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.1994.tb00342.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.1994.tb00342.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3970264$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7910936$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cirillo, Jeffrey D.</creatorcontrib><creatorcontrib>Weisbrod, Torin R.</creatorcontrib><creatorcontrib>Pascopella, Lisa</creatorcontrib><creatorcontrib>Bloom, Barry R.</creatorcontrib><creatorcontrib>Jacobs, William R.</creatorcontrib><title>Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Diaminopimelic acid (DAP) is a major component of the peptidoglycan layer of the mycobacterial cell wall. The mycobacterial cell wall has been implicated as a potential virulence factor and is highly immunogenic. The pathway for biosynthesis of DAP may serve as a target in the design of antimycobacterial agents and construction of in vivo selection systems. Despite its significance, this biosynthetic pathway is poorly understood in mycobacteria. In order to develop a better understanding of mycobacterial DAP biosynthesis, the aspartate semialdehyde dehydrogenase (asd) genes of Mycobacterium smegmatis, bacille Calmette‐Guerin (BCG), Mycobacterium avium, Mycobacterium leprae, and Mycobacterium tuberculosis were isolated. The M. smegmatis asd gene was isolated by complementation in Escherichia coli. This gene was then used to isolate the asd genes from other mycobacteria. The asd‐complemening fragments from BCG and M. smegmatis were sequenced. An open reading frame upstream of the mycobacterial asd gene was identified as the mycobacterial aspartokinase gene (ask). Primer extension analysis revealed that the only transcriptional start in this region is found 5’of the ask gene. This observation indicates that the mycobacterial ask and asd genes are in an operon.</description><subject>Amino Acid Sequence</subject><subject>Aspartate Kinase - genetics</subject><subject>Aspartate-Semialdehyde Dehydrogenase - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Consensus Sequence</subject><subject>Diaminopimelic Acid - metabolism</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetic Complementation Test</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mycobacterium - enzymology</subject><subject>Mycobacterium - genetics</subject><subject>Mycobacterium avium</subject><subject>Mycobacterium smegmatis</subject><subject>Mycobacterium tuberculosis</subject><subject>Open Reading Frames</subject><subject>Operon</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkUFr3DAQhUVJSDdpf0LBlNCbHcmyZKuHQliaZCEhlxZ6E2Np3PXWtraSl2bz66tdm72W6jIw75unGR4hHxnNWHw3m4xxKdJciSpjShXZWFPKizx7eUMWJ-mMLKgSNOVV_uMtuQxhQynjVPILclEqRhWXC7JbBdfB2LohgcEmZg0ezIi-fZ2arknGNSYQtuBH96sdIOCRnDowYhKwb6GzuN5bTI7Fu594BN0WfTRpvOuTfm9cPXnDO3LeQBfw_VyvyPe7r9-WD-nj8_1qefuYmkIJlbK64lbapok38YqVohac5iavEcDQxsjCAi0tClMDYl0WvIxnARVWVVCYkl-RT5Pv1rvfOwyj7ttgsOtgQLcLupSCVkVF_wkyGffhnEXw8wQa70Lw2Oitb3vwe82oPoSjN_qQgD4koA_h6Dkc_RKHP8y_7Ooe7Wl0TiPq17MOwUDXeBhMG04YVyXNZRGxLxP2p-1w_x8L6KenlcwV_wu-RK9c</recordid><startdate>199402</startdate><enddate>199402</enddate><creator>Cirillo, Jeffrey D.</creator><creator>Weisbrod, Torin R.</creator><creator>Pascopella, Lisa</creator><creator>Bloom, Barry R.</creator><creator>Jacobs, William R.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>199402</creationdate><title>Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria</title><author>Cirillo, Jeffrey D. ; Weisbrod, Torin R. ; Pascopella, Lisa ; Bloom, Barry R. ; Jacobs, William R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4959-1b83d6dff13638175b5302c2beaac0fc64da07de5cbaeeb7437109a05d98a4c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Aspartate Kinase - genetics</topic><topic>Aspartate-Semialdehyde Dehydrogenase - genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Consensus Sequence</topic><topic>Diaminopimelic Acid - metabolism</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetic Complementation Test</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mycobacterium - enzymology</topic><topic>Mycobacterium - genetics</topic><topic>Mycobacterium avium</topic><topic>Mycobacterium smegmatis</topic><topic>Mycobacterium tuberculosis</topic><topic>Open Reading Frames</topic><topic>Operon</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cirillo, Jeffrey D.</creatorcontrib><creatorcontrib>Weisbrod, Torin R.</creatorcontrib><creatorcontrib>Pascopella, Lisa</creatorcontrib><creatorcontrib>Bloom, Barry R.</creatorcontrib><creatorcontrib>Jacobs, William R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cirillo, Jeffrey D.</au><au>Weisbrod, Torin R.</au><au>Pascopella, Lisa</au><au>Bloom, Barry R.</au><au>Jacobs, William R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1994-02</date><risdate>1994</risdate><volume>11</volume><issue>4</issue><spage>629</spage><epage>639</epage><pages>629-639</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Diaminopimelic acid (DAP) is a major component of the peptidoglycan layer of the mycobacterial cell wall. The mycobacterial cell wall has been implicated as a potential virulence factor and is highly immunogenic. The pathway for biosynthesis of DAP may serve as a target in the design of antimycobacterial agents and construction of in vivo selection systems. Despite its significance, this biosynthetic pathway is poorly understood in mycobacteria. In order to develop a better understanding of mycobacterial DAP biosynthesis, the aspartate semialdehyde dehydrogenase (asd) genes of Mycobacterium smegmatis, bacille Calmette‐Guerin (BCG), Mycobacterium avium, Mycobacterium leprae, and Mycobacterium tuberculosis were isolated. The M. smegmatis asd gene was isolated by complementation in Escherichia coli. This gene was then used to isolate the asd genes from other mycobacteria. The asd‐complemening fragments from BCG and M. smegmatis were sequenced. An open reading frame upstream of the mycobacterial asd gene was identified as the mycobacterial aspartokinase gene (ask). Primer extension analysis revealed that the only transcriptional start in this region is found 5’of the ask gene. This observation indicates that the mycobacterial ask and asd genes are in an operon.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7910936</pmid><doi>10.1111/j.1365-2958.1994.tb00342.x</doi><tpages>11</tpages></addata></record> |
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| ispartof | Molecular microbiology, 1994-02, Vol.11 (4), p.629-639 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amino Acid Sequence Aspartate Kinase - genetics Aspartate-Semialdehyde Dehydrogenase - genetics Bacterial Proteins - genetics Bacteriology Base Sequence Biological and medical sciences Consensus Sequence Diaminopimelic Acid - metabolism Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic Complementation Test Metabolism. Enzymes Microbiology Molecular Sequence Data Mycobacterium - enzymology Mycobacterium - genetics Mycobacterium avium Mycobacterium smegmatis Mycobacterium tuberculosis Open Reading Frames Operon Sequence Alignment Sequence Homology, Amino Acid |
| title | Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria |
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