Affinity labeling of NADP+-specific isocitrate dehydrogenase by a new fluorescent nucleotide analog, 2-[(4-bromo-2,3-dioxobutyl)thio]-1,N6-ethenoadenosine 2',5'-bisphosphate

A new reactive fluorescent adenine nucleotide analogue has been synthesized and characterized: 2-[(4-bromo-2,3-dioxobutyl)thio]-1,N6-ethenoadenosine 2',5'-bisphosphate (BDB-T epsilon ADP). This compound reacts irreversibly with NADP+-specific isocitrate dehydrogenase. Biphasic kinetics of...

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Veröffentlicht in:	Biochemistry (Easton) 1985-09, Vol.24 (20), p.5367-5377
Hauptverfasser:	Bailey, Jerome M, Colman, Roberta F
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Diphosphate - analogs & derivatives Adenosine Diphosphate - chemical synthesis Adenosine Diphosphate - pharmacology Affinity Labels - pharmacology Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Enzymes and enzyme inhibitors Fluorescent Dyes - pharmacology Fundamental and applied biological sciences. Psychology Indicators and Reagents Isocitrate Dehydrogenase - isolation & purification Isocitrate Dehydrogenase - metabolism Kinetics Macromolecular Substances Molecular Weight Myocardium - enzymology NADP Oxidoreductases Spectrometry, Fluorescence Swine
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