Binding of carbohydrates to solid supports: evaluation of a prototype system

Mono- and disaccharides were covalently and irreversibly bound to aminopolystyrene beads in good yield by heating in dilute aqueous solution. The degree and stability of sugar binding were determined by chemical and radiochemical methods and the accessibility of the bound sugars was demonstrated by...

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Veröffentlicht in:Glycoconjugate journal 1994-02, Vol.11 (1), p.11-16
Hauptverfasser: Tweeddale, H J, Batley, M, Mei, X G, Redmond, J W
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container_title Glycoconjugate journal
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creator Tweeddale, H J
Batley, M
Mei, X G
Redmond, J W
description Mono- and disaccharides were covalently and irreversibly bound to aminopolystyrene beads in good yield by heating in dilute aqueous solution. The degree and stability of sugar binding were determined by chemical and radiochemical methods and the accessibility of the bound sugars was demonstrated by exoglycosidase hydrolysis and by an enzyme-linked lectin-binding assay using Concanavalin A.
doi_str_mv 10.1007/BF00732427
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source MEDLINE; SpringerLink Journals - AutoHoldings
subjects Chromatography - methods
Glucose - analogs & derivatives
Maltose - analogs & derivatives
Oxidation-Reduction
Polystyrenes - chemistry
title Binding of carbohydrates to solid supports: evaluation of a prototype system
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