The synthesis of lipids from [1- 14C]acetate by isolated rat brain capillaries
Lipid biosynthesis was investigated in isolated cerebral microvessels obtained from adult Sprague-Dawley rats using [1- 14C]acetate as precursor. All lipid classes were labelled by [1- 14C]acetate. Neutral lipids incorporated about 50% of radiolabelled acetate, among which free fatty acids and trigl...
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| Veröffentlicht in: | Neuroscience letters 1985-11, Vol.62 (1), p.143-147 |
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| container_title | Neuroscience letters |
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| creator | Homayoun, P. Roux, F. Niel, E. Bourre, J.M. |
| description | Lipid biosynthesis was investigated in isolated cerebral microvessels obtained from adult Sprague-Dawley rats using [1-
14C]acetate as precursor. All lipid classes were labelled by [1-
14C]acetate. Neutral lipids incorporated about 50% of radiolabelled acetate, among which free fatty acids and triglycerids showed the highest level of incorporation. Moreover, about 4% of radioactivity was found in cholesterol fraction. In phospholipid fraction, phosphatidylcholine and phosphatidylethanolamine were the main radiolabelled phospholipids. [1-
14C]acetate was also incorporated into sulphatides and cerebrosides. The presence of bovine serum albumin in incubation medium modified the percentage of incorporation in different lipid fractions. |
| doi_str_mv | 10.1016/0304-3940(85)90298-8 |
| format | Article |
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14C]acetate as precursor. All lipid classes were labelled by [1-
14C]acetate. Neutral lipids incorporated about 50% of radiolabelled acetate, among which free fatty acids and triglycerids showed the highest level of incorporation. Moreover, about 4% of radioactivity was found in cholesterol fraction. In phospholipid fraction, phosphatidylcholine and phosphatidylethanolamine were the main radiolabelled phospholipids. [1-
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14C]acetate as precursor. All lipid classes were labelled by [1-
14C]acetate. Neutral lipids incorporated about 50% of radiolabelled acetate, among which free fatty acids and triglycerids showed the highest level of incorporation. Moreover, about 4% of radioactivity was found in cholesterol fraction. In phospholipid fraction, phosphatidylcholine and phosphatidylethanolamine were the main radiolabelled phospholipids. [1-
14C]acetate was also incorporated into sulphatides and cerebrosides. The presence of bovine serum albumin in incubation medium modified the percentage of incorporation in different lipid fractions.</description><subject>Acetates - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>brain capillary</subject><subject>Capillaries - anatomy & histology</subject><subject>Capillaries - metabolism</subject><subject>Carbon Radioisotopes</subject><subject>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</subject><subject>Cerebrovascular Circulation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycolipids - biosynthesis</subject><subject>In Vitro Techniques</subject><subject>lipid synthesis</subject><subject>Lipids - biosynthesis</subject><subject>Male</subject><subject>Methods</subject><subject>Microscopy, Phase-Contrast</subject><subject>Phospholipids - biosynthesis</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFqGzEQhkVJcNykb9CADiG0h02llVYrXQLBtE0gpJfkFIKYlWaxynrX1awDfvuua-NjTzMw3_z8fIx9luJGCmm-CSV0oZwWX2z11YnS2cJ-YHNp67KoXV2esPkROWMfiX4LISpZ6RmbKSeM1nrOnp6XyGnbj0ukRHxoeZfWKRJv87Dir7LgUi_eIOAII_JmyxMN3bRGnmHkTYbU8wDr1HWQE9IFO22hI_x0mOfs5cf358V98fjr58Pi7rEIypqxaCrtAJSLWjsbTHQylFFpY0KlAhgZDEQlFZYlNLWsULZ1K007rQFEiUKds-t97joPfzZIo18lCji16HHYkK-NdkbWbgL1Hgx5IMrY-nVOK8hbL4XfafQ7R37nyNvK_9Po7fR2ecjfNCuMx6eDt-l-dbgDBejaDH1IdMSsEcKaesJu9xhOLt4TZk8hYR8wpoxh9HFI_-_xFxuNjMw</recordid><startdate>19851120</startdate><enddate>19851120</enddate><creator>Homayoun, P.</creator><creator>Roux, F.</creator><creator>Niel, E.</creator><creator>Bourre, J.M.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19851120</creationdate><title>The synthesis of lipids from [1- 14C]acetate by isolated rat brain capillaries</title><author>Homayoun, P. ; Roux, F. ; Niel, E. ; Bourre, J.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-b549aa39d4498c6d91c2d3466c53ca61c6ad313e22ab715e1f7f16f715ca02e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Acetates - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>brain capillary</topic><topic>Capillaries - anatomy & histology</topic><topic>Capillaries - metabolism</topic><topic>Carbon Radioisotopes</topic><topic>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</topic><topic>Cerebrovascular Circulation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycolipids - biosynthesis</topic><topic>In Vitro Techniques</topic><topic>lipid synthesis</topic><topic>Lipids - biosynthesis</topic><topic>Male</topic><topic>Methods</topic><topic>Microscopy, Phase-Contrast</topic><topic>Phospholipids - biosynthesis</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Homayoun, P.</creatorcontrib><creatorcontrib>Roux, F.</creatorcontrib><creatorcontrib>Niel, E.</creatorcontrib><creatorcontrib>Bourre, J.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Homayoun, P.</au><au>Roux, F.</au><au>Niel, E.</au><au>Bourre, J.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The synthesis of lipids from [1- 14C]acetate by isolated rat brain capillaries</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1985-11-20</date><risdate>1985</risdate><volume>62</volume><issue>1</issue><spage>143</spage><epage>147</epage><pages>143-147</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Lipid biosynthesis was investigated in isolated cerebral microvessels obtained from adult Sprague-Dawley rats using [1-
14C]acetate as precursor. All lipid classes were labelled by [1-
14C]acetate. Neutral lipids incorporated about 50% of radiolabelled acetate, among which free fatty acids and triglycerids showed the highest level of incorporation. Moreover, about 4% of radioactivity was found in cholesterol fraction. In phospholipid fraction, phosphatidylcholine and phosphatidylethanolamine were the main radiolabelled phospholipids. [1-
14C]acetate was also incorporated into sulphatides and cerebrosides. The presence of bovine serum albumin in incubation medium modified the percentage of incorporation in different lipid fractions.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>3906444</pmid><doi>10.1016/0304-3940(85)90298-8</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3940 |
| ispartof | Neuroscience letters, 1985-11, Vol.62 (1), p.143-147 |
| issn | 0304-3940 1872-7972 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Acetates - metabolism Animals Biological and medical sciences brain capillary Capillaries - anatomy & histology Capillaries - metabolism Carbon Radioisotopes Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges Cerebrovascular Circulation Female Fundamental and applied biological sciences. Psychology Glycolipids - biosynthesis In Vitro Techniques lipid synthesis Lipids - biosynthesis Male Methods Microscopy, Phase-Contrast Phospholipids - biosynthesis rat Rats Rats, Inbred Strains Vertebrates: nervous system and sense organs |
| title | The synthesis of lipids from [1- 14C]acetate by isolated rat brain capillaries |
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