Steady state models of spore cell metabolism in Dictyostelium discoideum
Young sorocarps (consisting of a mass of spore cells resting on a stalk) were exposed to low levels of [U-14C]glucose and the spore cells were rapidly separated from stalk cells. Metabolites were isolated from spores and their specific radioactivities compared to these metabolites isolated from the...
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| Veröffentlicht in: | The Journal of biological chemistry 1985-12, Vol.260 (28), p.15325-15331 |
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| container_end_page | 15331 |
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| container_issue | 28 |
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| container_title | The Journal of biological chemistry |
| container_volume | 260 |
| creator | Chiew, Y Y Reimers, J M Wright, B E |
| description | Young sorocarps (consisting of a mass of spore cells resting on a stalk) were exposed to low levels of [U-14C]glucose and the spore cells were rapidly separated from stalk cells. Metabolites were isolated from spores and their specific radioactivities compared to these metabolites isolated from the whole organism; i.e. spore plus stalk cells. Based on these data, known reaction rates, and metabolite concentrations, highly constrained steady state models of metabolism in spore and stalk cells were constructed. Direct evidence has been obtained which substantiates earlier predictions regarding cell permeability, the distribution of specific metabolites, and the location of reactions in vivo. |
| doi_str_mv | 10.1016/S0021-9258(18)95739-X |
| format | Article |
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Metabolites were isolated from spores and their specific radioactivities compared to these metabolites isolated from the whole organism; i.e. spore plus stalk cells. Based on these data, known reaction rates, and metabolite concentrations, highly constrained steady state models of metabolism in spore and stalk cells were constructed. Direct evidence has been obtained which substantiates earlier predictions regarding cell permeability, the distribution of specific metabolites, and the location of reactions in vivo.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)95739-X</identifier><identifier>PMID: 4066672</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Biochemistry. Physiology. Immunology. 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Psychology</subject><subject>Glucose - metabolism</subject><subject>Models, Biological</subject><subject>Permeability</subject><subject>Protozoa</subject><subject>Spores, Fungal - metabolism</subject><subject>Time Factors</subject><subject>Trypan Blue - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtvEzEURi0EKmnhJ1TyAiG6GPBzxl6hqjyKVIlFQcrO8uMOMRrHYewB5d_jNlFY4s1d3PP5fjoIXVLylhLav7snhNFOM6neUHWl5cB1t36CVpQo3nFJ10_R6oQ8R-el_CTtCU3P0Jkgfd8PbIVu7yvYsMel2go45QBTwXnEZZdnwB6mCSeo1uUploTjFn-Ivu5zqTDFJeEQi88xwJJeoGejnQq8PM4L9P3Tx283t93d189fbq7vOi96WTsPioIjTiobQHgnNAjOuVWM-NGCo1aDdTo0jHlFAKhwVPOBcNC6HwZ-gV4f_t3N-dcCpZrUOrSedgt5KWboheac0QbKA-jnXMoMo9nNMdl5bygxDwbNo0HzoMdQZR4NmnXLXR4PLC5BOKWOytr-1XFvi7fTONutj-WEKanEoMg_bBN_bP7EGYyL2W8gGdYTw9pJyZls2PsD1rzD7wizKT7C1kNoEV9NyPE_ff8CDEWZ_Q</recordid><startdate>19851205</startdate><enddate>19851205</enddate><creator>Chiew, Y Y</creator><creator>Reimers, J M</creator><creator>Wright, B E</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19851205</creationdate><title>Steady state models of spore cell metabolism in Dictyostelium discoideum</title><author>Chiew, Y Y ; Reimers, J M ; Wright, B E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-ce81eb0b58ade4cb49e4333a820cfaeb1a9eab9de812c80ee14b193703e996773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Biochemistry. 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Psychology</topic><topic>Glucose - metabolism</topic><topic>Models, Biological</topic><topic>Permeability</topic><topic>Protozoa</topic><topic>Spores, Fungal - metabolism</topic><topic>Time Factors</topic><topic>Trypan Blue - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chiew, Y Y</creatorcontrib><creatorcontrib>Reimers, J M</creatorcontrib><creatorcontrib>Wright, B E</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiew, Y Y</au><au>Reimers, J M</au><au>Wright, B E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Steady state models of spore cell metabolism in Dictyostelium discoideum</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1985-12-05</date><risdate>1985</risdate><volume>260</volume><issue>28</issue><spage>15325</spage><epage>15331</epage><pages>15325-15331</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Young sorocarps (consisting of a mass of spore cells resting on a stalk) were exposed to low levels of [U-14C]glucose and the spore cells were rapidly separated from stalk cells. 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| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1985-12, Vol.260 (28), p.15325-15331 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Computers Dictyostelium - metabolism Fundamental and applied biological sciences. Psychology Glucose - metabolism Models, Biological Permeability Protozoa Spores, Fungal - metabolism Time Factors Trypan Blue - metabolism |
| title | Steady state models of spore cell metabolism in Dictyostelium discoideum |
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