Screening of monoclonal antibodies recognizing oncofetal antigens for isolation of trophoblasts from maternal blood for prenatal diagnosis
Many monoclonal antibodies have been produced against tumour‐associated cell surface antigens for cancer therapy. They have therefore been selected for minimal reactivity with normal tissues and in particular for lack of binding to blood cells or serum components. Many of the antigens recognized are...
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| Veröffentlicht in: | Prenatal diagnosis 1994-02, Vol.14 (2), p.131-140 |
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| container_title | Prenatal diagnosis |
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| creator | Durrant, L. G. McDowell, K. M. Holmes, R. A. Liu, D. T. Y. |
| description | Many monoclonal antibodies have been produced against tumour‐associated cell surface antigens for cancer therapy. They have therefore been selected for minimal reactivity with normal tissues and in particular for lack of binding to blood cells or serum components. Many of the antigens recognized are of fetal origin. These monoclonal antibodies may therefore be ideal candidates to recognize and sort fetal trophoblasts from maternal blood for prenatal diagnosis of genetic abnormalities. A panel of 19 anti‐tumour antibodies were therefore screened for reactivity with early trimester placenta and two, 340 and 154, were shown to stain trophoblasts. If MAb 340 is linked to magnetic beads, it can efficiently sort trophoblast cell lines from whole blood. |
| doi_str_mv | 10.1002/pd.1970140209 |
| format | Article |
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G. ; McDowell, K. M. ; Holmes, R. A. ; Liu, D. T. Y.</creator><creatorcontrib>Durrant, L. G. ; McDowell, K. M. ; Holmes, R. A. ; Liu, D. T. Y.</creatorcontrib><description>Many monoclonal antibodies have been produced against tumour‐associated cell surface antigens for cancer therapy. They have therefore been selected for minimal reactivity with normal tissues and in particular for lack of binding to blood cells or serum components. Many of the antigens recognized are of fetal origin. These monoclonal antibodies may therefore be ideal candidates to recognize and sort fetal trophoblasts from maternal blood for prenatal diagnosis of genetic abnormalities. A panel of 19 anti‐tumour antibodies were therefore screened for reactivity with early trimester placenta and two, 340 and 154, were shown to stain trophoblasts. If MAb 340 is linked to magnetic beads, it can efficiently sort trophoblast cell lines from whole blood.</description><identifier>ISSN: 0197-3851</identifier><identifier>EISSN: 1097-0223</identifier><identifier>DOI: 10.1002/pd.1970140209</identifier><identifier>PMID: 8183849</identifier><identifier>CODEN: PRDIDM</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Antibodies, Monoclonal ; Antibody Specificity ; Antigens, Neoplasm - immunology ; Biological and medical sciences ; Cell Separation - methods ; Female ; Fluorescent Antibody Technique ; Gynecology. Andrology. Obstetrics ; Humans ; Immunoenzyme Techniques ; Immunohistochemistry ; Magnetics ; Management. Prenatal diagnosis ; Medical sciences ; Microspheres ; monoclonal antibodies ; Pregnancy ; Pregnancy. Fetus. Placenta ; Prenatal Diagnosis ; prenatal screening ; Trophoblasts ; Trophoblasts - cytology ; Trophoblasts - immunology ; Tumor Cells, Cultured</subject><ispartof>Prenatal diagnosis, 1994-02, Vol.14 (2), p.131-140</ispartof><rights>Copyright © 1994 John Wiley & Sons, Ltd.</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4349-776d0296097fd482c5a9051c00acc1088c8f66cb5985d836f4c993f3120550a53</citedby><cites>FETCH-LOGICAL-c4349-776d0296097fd482c5a9051c00acc1088c8f66cb5985d836f4c993f3120550a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpd.1970140209$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpd.1970140209$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3945981$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8183849$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Durrant, L. G.</creatorcontrib><creatorcontrib>McDowell, K. M.</creatorcontrib><creatorcontrib>Holmes, R. A.</creatorcontrib><creatorcontrib>Liu, D. T. Y.</creatorcontrib><title>Screening of monoclonal antibodies recognizing oncofetal antigens for isolation of trophoblasts from maternal blood for prenatal diagnosis</title><title>Prenatal diagnosis</title><addtitle>Prenat. Diagn</addtitle><description>Many monoclonal antibodies have been produced against tumour‐associated cell surface antigens for cancer therapy. They have therefore been selected for minimal reactivity with normal tissues and in particular for lack of binding to blood cells or serum components. Many of the antigens recognized are of fetal origin. These monoclonal antibodies may therefore be ideal candidates to recognize and sort fetal trophoblasts from maternal blood for prenatal diagnosis of genetic abnormalities. A panel of 19 anti‐tumour antibodies were therefore screened for reactivity with early trimester placenta and two, 340 and 154, were shown to stain trophoblasts. If MAb 340 is linked to magnetic beads, it can efficiently sort trophoblast cell lines from whole blood.</description><subject>Antibodies, Monoclonal</subject><subject>Antibody Specificity</subject><subject>Antigens, Neoplasm - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell Separation - methods</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Immunohistochemistry</subject><subject>Magnetics</subject><subject>Management. Prenatal diagnosis</subject><subject>Medical sciences</subject><subject>Microspheres</subject><subject>monoclonal antibodies</subject><subject>Pregnancy</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>Prenatal Diagnosis</subject><subject>prenatal screening</subject><subject>Trophoblasts</subject><subject>Trophoblasts - cytology</subject><subject>Trophoblasts - immunology</subject><subject>Tumor Cells, Cultured</subject><issn>0197-3851</issn><issn>1097-0223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EKkvhyBEpB8Qtxf_i2EdUoIAqqNQierMcx14MjifYWUH5CHxqkm60iAuc5vB-82aeHkKPCT4hGNPnY39CVIsJxxSrO2hDsGprTCm7izZ4VmomG3IfPSjly4xLqtojdCSJZJKrDfp1abNzKaRtBb4aIIGNkEysTJpCB31wpcrOwjaFn7dQsuDdtAJbl0rlIVehQDRTgLS4TBnGz9BFU6ZZzTBUg5lcXly7CNDfbozZJbP49MFsE5RQHqJ73sTiHq3zGH18_erq9E19_uHs7emL89pyxlXdtqLHVIk5pu-5pLYxCjfEYmysJVhKK70QtmuUbHrJhOdWKeYZobhpsGnYMXq29x0zfNu5MukhFOtiNMnBruhWcEUlx_8FiZBYSEpmsN6DNkMp2Xk95jCYfKMJ1ktJeuz1n5Jm_slqvOsG1x_otZVZf7rqplgTfTbJhnLAmOJzuOVsu8e-h-hu_n1TX7z864H14VAm9-OwafJXLVrWNvrT-zP97opdU3Fxqa_Zb2d3uuU</recordid><startdate>199402</startdate><enddate>199402</enddate><creator>Durrant, L. G.</creator><creator>McDowell, K. M.</creator><creator>Holmes, R. A.</creator><creator>Liu, D. T. Y.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199402</creationdate><title>Screening of monoclonal antibodies recognizing oncofetal antigens for isolation of trophoblasts from maternal blood for prenatal diagnosis</title><author>Durrant, L. G. ; McDowell, K. M. ; Holmes, R. A. ; Liu, D. T. Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4349-776d0296097fd482c5a9051c00acc1088c8f66cb5985d836f4c993f3120550a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Antibodies, Monoclonal</topic><topic>Antibody Specificity</topic><topic>Antigens, Neoplasm - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell Separation - methods</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Immunohistochemistry</topic><topic>Magnetics</topic><topic>Management. Prenatal diagnosis</topic><topic>Medical sciences</topic><topic>Microspheres</topic><topic>monoclonal antibodies</topic><topic>Pregnancy</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>Prenatal Diagnosis</topic><topic>prenatal screening</topic><topic>Trophoblasts</topic><topic>Trophoblasts - cytology</topic><topic>Trophoblasts - immunology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Durrant, L. G.</creatorcontrib><creatorcontrib>McDowell, K. M.</creatorcontrib><creatorcontrib>Holmes, R. A.</creatorcontrib><creatorcontrib>Liu, D. T. Y.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Prenatal diagnosis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Durrant, L. G.</au><au>McDowell, K. M.</au><au>Holmes, R. A.</au><au>Liu, D. T. Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening of monoclonal antibodies recognizing oncofetal antigens for isolation of trophoblasts from maternal blood for prenatal diagnosis</atitle><jtitle>Prenatal diagnosis</jtitle><addtitle>Prenat. Diagn</addtitle><date>1994-02</date><risdate>1994</risdate><volume>14</volume><issue>2</issue><spage>131</spage><epage>140</epage><pages>131-140</pages><issn>0197-3851</issn><eissn>1097-0223</eissn><coden>PRDIDM</coden><abstract>Many monoclonal antibodies have been produced against tumour‐associated cell surface antigens for cancer therapy. They have therefore been selected for minimal reactivity with normal tissues and in particular for lack of binding to blood cells or serum components. Many of the antigens recognized are of fetal origin. These monoclonal antibodies may therefore be ideal candidates to recognize and sort fetal trophoblasts from maternal blood for prenatal diagnosis of genetic abnormalities. A panel of 19 anti‐tumour antibodies were therefore screened for reactivity with early trimester placenta and two, 340 and 154, were shown to stain trophoblasts. If MAb 340 is linked to magnetic beads, it can efficiently sort trophoblast cell lines from whole blood.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>8183849</pmid><doi>10.1002/pd.1970140209</doi><tpages>10</tpages></addata></record> |
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| ispartof | Prenatal diagnosis, 1994-02, Vol.14 (2), p.131-140 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Antibodies, Monoclonal Antibody Specificity Antigens, Neoplasm - immunology Biological and medical sciences Cell Separation - methods Female Fluorescent Antibody Technique Gynecology. Andrology. Obstetrics Humans Immunoenzyme Techniques Immunohistochemistry Magnetics Management. Prenatal diagnosis Medical sciences Microspheres monoclonal antibodies Pregnancy Pregnancy. Fetus. Placenta Prenatal Diagnosis prenatal screening Trophoblasts Trophoblasts - cytology Trophoblasts - immunology Tumor Cells, Cultured |
| title | Screening of monoclonal antibodies recognizing oncofetal antigens for isolation of trophoblasts from maternal blood for prenatal diagnosis |
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