CNS regulation of blood lactate concentration in anesthetized rats
This study evaluated the effect of stimulating the central nervous system (CNS) with neostigmine, an inhibitor of acetylcholinesterase, on the blood lactate concentration in fed rats and in rats fasted for 48 hours. After the rat was anesthetized with pentobarbital, neostigmine was stereotaxically i...
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| Veröffentlicht in: | Life sciences (1973) 1994, Vol.54 (20), p.1491-1499 |
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| container_title | Life sciences (1973) |
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| creator | Watanabe, Genichi Ishiguro, Toshiaki Miura, Hisayuki Uemura, Kazumasa Hiyoshi, Yasuo Ozawa, Kuniaki Nonogaki, Katsunori Tamagawa, Tatsuo Sakamoto, Nobuo Iguchi, Akihisa |
| description | This study evaluated the effect of stimulating the central nervous system (CNS) with neostigmine, an inhibitor of acetylcholinesterase, on the blood lactate concentration in fed rats and in rats fasted for 48 hours. After the rat was anesthetized with pentobarbital, neostigmine was stereotaxically injected into the third cerebral ventricle. In fed rats, the central injection of neostigmine significantly increased the blood lactate level, while concomitantly increasing plasma glucagon, epinephrine and norepinephrine concentrations. Constant infusion of somatostatin throughout the experiments, to inhibit glucagon secretion from the pancreas, did not affect alterations in blood lactate by central injection of neostigmine. In adrenodemedullated rats, CNS-stimulation by neostigmine still increased plasma norepinephrine significantly, however, the alteration in blood lactate was only one-third of that in intact rats. Intraperitoneal propranolol, but not phentolamine, prevented the rise in lactate. Neostigmine increased lactate in fasted rats as well as in fed rats. We conclude that in anesthetized rats, stimulation of the CNS by neostigmine increases blood lactate mainly through circulating epinephrine and partially through circulating norepinephrine or direct sympathetic nervous stimulation; glucagon does not appear to be involved in the increase in blood lactate. |
| doi_str_mv | 10.1016/0024-3205(94)90016-7 |
| format | Article |
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After the rat was anesthetized with pentobarbital, neostigmine was stereotaxically injected into the third cerebral ventricle. In fed rats, the central injection of neostigmine significantly increased the blood lactate level, while concomitantly increasing plasma glucagon, epinephrine and norepinephrine concentrations. Constant infusion of somatostatin throughout the experiments, to inhibit glucagon secretion from the pancreas, did not affect alterations in blood lactate by central injection of neostigmine. In adrenodemedullated rats, CNS-stimulation by neostigmine still increased plasma norepinephrine significantly, however, the alteration in blood lactate was only one-third of that in intact rats. Intraperitoneal propranolol, but not phentolamine, prevented the rise in lactate. Neostigmine increased lactate in fasted rats as well as in fed rats. We conclude that in anesthetized rats, stimulation of the CNS by neostigmine increases blood lactate mainly through circulating epinephrine and partially through circulating norepinephrine or direct sympathetic nervous stimulation; glucagon does not appear to be involved in the increase in blood lactate.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/0024-3205(94)90016-7</identifier><identifier>PMID: 7910651</identifier><identifier>CODEN: LIFSAK</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Anesthesia, General ; Animals ; Biological and medical sciences ; Blood Glucose - drug effects ; Blood Glucose - metabolism ; central nervous system ; Cerebral Ventricles - drug effects ; Cerebral Ventricles - physiology ; Eating ; epinephrine ; Epinephrine - blood ; Fasting ; Fundamental and applied biological sciences. Psychology ; glucagon ; Glucagon - blood ; Glucagon - metabolism ; Infusions, Intravenous ; Injections, Intraventricular ; Intermediate and energetic metabolism ; lactate ; Lactates - blood ; Lactic Acid ; Male ; Metabolisms and neurohumoral controls ; neostigmine ; Neostigmine - administration & dosage ; Neostigmine - pharmacology ; norepinephrine ; Norepinephrine - pharmacology ; Pentobarbital ; Phentolamine - pharmacology ; Propranolol - pharmacology ; Rats ; Rats, Wistar ; Reference Values ; Somatostatin - administration & dosage ; Somatostatin - pharmacology ; Stereotaxic Techniques ; Time Factors ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Life sciences (1973), 1994, Vol.54 (20), p.1491-1499</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-e76cffeead5b99e7c66f9585c16702c456e0ce20a2496cde4f5969f36cfe040b3</citedby><cites>FETCH-LOGICAL-c417t-e76cffeead5b99e7c66f9585c16702c456e0ce20a2496cde4f5969f36cfe040b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0024-3205(94)90016-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4137306$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7910651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watanabe, Genichi</creatorcontrib><creatorcontrib>Ishiguro, Toshiaki</creatorcontrib><creatorcontrib>Miura, Hisayuki</creatorcontrib><creatorcontrib>Uemura, Kazumasa</creatorcontrib><creatorcontrib>Hiyoshi, Yasuo</creatorcontrib><creatorcontrib>Ozawa, Kuniaki</creatorcontrib><creatorcontrib>Nonogaki, Katsunori</creatorcontrib><creatorcontrib>Tamagawa, Tatsuo</creatorcontrib><creatorcontrib>Sakamoto, Nobuo</creatorcontrib><creatorcontrib>Iguchi, Akihisa</creatorcontrib><title>CNS regulation of blood lactate concentration in anesthetized rats</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>This study evaluated the effect of stimulating the central nervous system (CNS) with neostigmine, an inhibitor of acetylcholinesterase, on the blood lactate concentration in fed rats and in rats fasted for 48 hours. After the rat was anesthetized with pentobarbital, neostigmine was stereotaxically injected into the third cerebral ventricle. In fed rats, the central injection of neostigmine significantly increased the blood lactate level, while concomitantly increasing plasma glucagon, epinephrine and norepinephrine concentrations. Constant infusion of somatostatin throughout the experiments, to inhibit glucagon secretion from the pancreas, did not affect alterations in blood lactate by central injection of neostigmine. In adrenodemedullated rats, CNS-stimulation by neostigmine still increased plasma norepinephrine significantly, however, the alteration in blood lactate was only one-third of that in intact rats. Intraperitoneal propranolol, but not phentolamine, prevented the rise in lactate. Neostigmine increased lactate in fasted rats as well as in fed rats. We conclude that in anesthetized rats, stimulation of the CNS by neostigmine increases blood lactate mainly through circulating epinephrine and partially through circulating norepinephrine or direct sympathetic nervous stimulation; glucagon does not appear to be involved in the increase in blood lactate.</description><subject>Anesthesia, General</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - drug effects</subject><subject>Blood Glucose - metabolism</subject><subject>central nervous system</subject><subject>Cerebral Ventricles - drug effects</subject><subject>Cerebral Ventricles - physiology</subject><subject>Eating</subject><subject>epinephrine</subject><subject>Epinephrine - blood</subject><subject>Fasting</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glucagon</subject><subject>Glucagon - blood</subject><subject>Glucagon - metabolism</subject><subject>Infusions, Intravenous</subject><subject>Injections, Intraventricular</subject><subject>Intermediate and energetic metabolism</subject><subject>lactate</subject><subject>Lactates - blood</subject><subject>Lactic Acid</subject><subject>Male</subject><subject>Metabolisms and neurohumoral controls</subject><subject>neostigmine</subject><subject>Neostigmine - administration & dosage</subject><subject>Neostigmine - pharmacology</subject><subject>norepinephrine</subject><subject>Norepinephrine - pharmacology</subject><subject>Pentobarbital</subject><subject>Phentolamine - pharmacology</subject><subject>Propranolol - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reference Values</subject><subject>Somatostatin - administration & dosage</subject><subject>Somatostatin - pharmacology</subject><subject>Stereotaxic Techniques</subject><subject>Time Factors</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1LAzEQhoMoWqv_QGEPInpYnezmo7kIWvwC0YN6Dml2opHtRpOtoL_e1JYe9RSY93mH4QkhexROKFBxClCxsq6AHyl2rCCPSrlGBnQkVQmiputksEK2yHZKbwDAuaw3yaZUFASnA3Ixvn8sIr7MWtP70BXBFZM2hKZoje1Nj4UNncWuj4vYd4XpMPWv2PtvbIo8Tjtkw5k24e7yHZLnq8un8U1593B9Oz6_Ky2jsi9RCuscomn4RCmUVgin-IhbKiRUlnGBYLECUzElbIPMcSWUq3MLgcGkHpLDxd73GD5m-Qg99cli2-aLwixpKZiqpBr9C1IxooryKoNsAdoYUoro9Hv0UxO_NAU9d6znAvVcoFZM_zrWMtf2l_tnkyk2q9JSas4PlrlJ1rQums76tMIYrWWdP2hIzhYYZmmfHqNO1mO23fiIttdN8H_f8QOalJdU</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Watanabe, Genichi</creator><creator>Ishiguro, Toshiaki</creator><creator>Miura, Hisayuki</creator><creator>Uemura, Kazumasa</creator><creator>Hiyoshi, Yasuo</creator><creator>Ozawa, Kuniaki</creator><creator>Nonogaki, Katsunori</creator><creator>Tamagawa, Tatsuo</creator><creator>Sakamoto, Nobuo</creator><creator>Iguchi, Akihisa</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>CNS regulation of blood lactate concentration in anesthetized rats</title><author>Watanabe, Genichi ; Ishiguro, Toshiaki ; Miura, Hisayuki ; Uemura, Kazumasa ; Hiyoshi, Yasuo ; Ozawa, Kuniaki ; Nonogaki, Katsunori ; Tamagawa, Tatsuo ; Sakamoto, Nobuo ; Iguchi, Akihisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-e76cffeead5b99e7c66f9585c16702c456e0ce20a2496cde4f5969f36cfe040b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Anesthesia, General</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - drug effects</topic><topic>Blood Glucose - metabolism</topic><topic>central nervous system</topic><topic>Cerebral Ventricles - drug effects</topic><topic>Cerebral Ventricles - physiology</topic><topic>Eating</topic><topic>epinephrine</topic><topic>Epinephrine - blood</topic><topic>Fasting</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glucagon</topic><topic>Glucagon - blood</topic><topic>Glucagon - metabolism</topic><topic>Infusions, Intravenous</topic><topic>Injections, Intraventricular</topic><topic>Intermediate and energetic metabolism</topic><topic>lactate</topic><topic>Lactates - blood</topic><topic>Lactic Acid</topic><topic>Male</topic><topic>Metabolisms and neurohumoral controls</topic><topic>neostigmine</topic><topic>Neostigmine - administration & dosage</topic><topic>Neostigmine - pharmacology</topic><topic>norepinephrine</topic><topic>Norepinephrine - pharmacology</topic><topic>Pentobarbital</topic><topic>Phentolamine - pharmacology</topic><topic>Propranolol - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reference Values</topic><topic>Somatostatin - administration & dosage</topic><topic>Somatostatin - pharmacology</topic><topic>Stereotaxic Techniques</topic><topic>Time Factors</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Watanabe, Genichi</creatorcontrib><creatorcontrib>Ishiguro, Toshiaki</creatorcontrib><creatorcontrib>Miura, Hisayuki</creatorcontrib><creatorcontrib>Uemura, Kazumasa</creatorcontrib><creatorcontrib>Hiyoshi, Yasuo</creatorcontrib><creatorcontrib>Ozawa, Kuniaki</creatorcontrib><creatorcontrib>Nonogaki, Katsunori</creatorcontrib><creatorcontrib>Tamagawa, Tatsuo</creatorcontrib><creatorcontrib>Sakamoto, Nobuo</creatorcontrib><creatorcontrib>Iguchi, Akihisa</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watanabe, Genichi</au><au>Ishiguro, Toshiaki</au><au>Miura, Hisayuki</au><au>Uemura, Kazumasa</au><au>Hiyoshi, Yasuo</au><au>Ozawa, Kuniaki</au><au>Nonogaki, Katsunori</au><au>Tamagawa, Tatsuo</au><au>Sakamoto, Nobuo</au><au>Iguchi, Akihisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CNS regulation of blood lactate concentration in anesthetized rats</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>1994</date><risdate>1994</risdate><volume>54</volume><issue>20</issue><spage>1491</spage><epage>1499</epage><pages>1491-1499</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><coden>LIFSAK</coden><abstract>This study evaluated the effect of stimulating the central nervous system (CNS) with neostigmine, an inhibitor of acetylcholinesterase, on the blood lactate concentration in fed rats and in rats fasted for 48 hours. After the rat was anesthetized with pentobarbital, neostigmine was stereotaxically injected into the third cerebral ventricle. In fed rats, the central injection of neostigmine significantly increased the blood lactate level, while concomitantly increasing plasma glucagon, epinephrine and norepinephrine concentrations. Constant infusion of somatostatin throughout the experiments, to inhibit glucagon secretion from the pancreas, did not affect alterations in blood lactate by central injection of neostigmine. In adrenodemedullated rats, CNS-stimulation by neostigmine still increased plasma norepinephrine significantly, however, the alteration in blood lactate was only one-third of that in intact rats. Intraperitoneal propranolol, but not phentolamine, prevented the rise in lactate. Neostigmine increased lactate in fasted rats as well as in fed rats. We conclude that in anesthetized rats, stimulation of the CNS by neostigmine increases blood lactate mainly through circulating epinephrine and partially through circulating norepinephrine or direct sympathetic nervous stimulation; glucagon does not appear to be involved in the increase in blood lactate.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>7910651</pmid><doi>10.1016/0024-3205(94)90016-7</doi><tpages>9</tpages></addata></record> |
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| subjects | Anesthesia, General Animals Biological and medical sciences Blood Glucose - drug effects Blood Glucose - metabolism central nervous system Cerebral Ventricles - drug effects Cerebral Ventricles - physiology Eating epinephrine Epinephrine - blood Fasting Fundamental and applied biological sciences. Psychology glucagon Glucagon - blood Glucagon - metabolism Infusions, Intravenous Injections, Intraventricular Intermediate and energetic metabolism lactate Lactates - blood Lactic Acid Male Metabolisms and neurohumoral controls neostigmine Neostigmine - administration & dosage Neostigmine - pharmacology norepinephrine Norepinephrine - pharmacology Pentobarbital Phentolamine - pharmacology Propranolol - pharmacology Rats Rats, Wistar Reference Values Somatostatin - administration & dosage Somatostatin - pharmacology Stereotaxic Techniques Time Factors Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | CNS regulation of blood lactate concentration in anesthetized rats |
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