$Identification of artifactual cathepsin D activity in cardiac subcellular fractions related to formation of an iron-EDTA complex$

Identification of artifactual cathepsin D activity in cardiac subcellular fractions related to formation of an iron-EDTA complex

The conventional assay procedure for cathepsin D (E.C.3.4.23.5) activity in tissue homogenates and subcellular fractions requires incubation with hemoglobin as substrate. Cathepsin D (CD) activity is calculated by determining the increase in absorbance at 280 nm after precipitation of all proteins w...

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Veröffentlicht in:	Journal of molecular and cellular cardiology 1985-10, Vol.17 (10), p.997-1003
Hauptverfasser:	Nandi, Dhirendra L., Lesch, Michael, Samarel, Allen M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cathepsin D Cathepsin D - metabolism Cattle Edetic Acid - isolation & purification Edetic Acid - metabolism Edetic Acid - pharmacology Enzymes and enzyme inhibitors Fe-EDTA complex Ferric Compounds - metabolism Ferric Compounds - pharmacology Fundamental and applied biological sciences. Psychology Heart Ventricles - enzymology Hemoglobins - metabolism Hydrolases Imminoprecipitation Iron - pharmacology Kinetics Lysosomal enzymes Myocardium - enzymology Rabbits Subcellular fractions Subcellular Fractions - enzymology
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