A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium
In this study, two transformation vectors (pMG101 and pMG103) for Phanerochaete chrysosporium were constructed, based on the ble phleomycin-resistance-encoding gene and a homologous histone H4 promoter. Transformation frequencies were 6–10 per μg of DNA. Transformed vector DNA could either exist as...
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| Veröffentlicht in: | Gene 1994-05, Vol.142 (2), p.237-241 |
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| container_title | Gene |
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| creator | Gessner, Matthias Raeder, Ute |
| description | In this study, two transformation vectors (pMG101 and pMG103) for
Phanerochaete chrysosporium were constructed, based on the
ble phleomycin-resistance-encoding gene and a homologous histone
H4 promoter. Transformation frequencies were 6–10 per μg of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stably integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding
H3 gene in the promoter fragment, becomes methylated, resulting in inactivation of
ble-dependent resistance. Plasmid pMG103, which lacks the
H3, does not show methylation. |
| doi_str_mv | 10.1016/0378-1119(94)90267-4 |
| format | Article |
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Phanerochaete chrysosporium were constructed, based on the
ble phleomycin-resistance-encoding gene and a homologous histone
H4 promoter. Transformation frequencies were 6–10 per μg of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stably integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding
H3 gene in the promoter fragment, becomes methylated, resulting in inactivation of
ble-dependent resistance. Plasmid pMG103, which lacks the
H3, does not show methylation.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(94)90267-4</identifier><identifier>PMID: 8194757</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>antibiotics ; Bacterial Proteins - genetics ; Basidiomycota - drug effects ; Basidiomycota - genetics ; Basidiomycota - growth & development ; Biological and medical sciences ; Biotechnology ; ble gene ; cytotoxic compounds ; DNA transformation ; DNA, Fungal - metabolism ; drug resistance ; Drug Resistance, Microbial - genetics ; filamentous fungus ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic Markers - genetics ; Genetic technics ; genetic transformation ; Genetic Vectors ; histones ; Histones - genetics ; in vitro selection ; Methods. Procedures. Technologies ; Methylation ; Phanerochaete chrysosporium ; Phleomycins - pharmacology ; plasmid vectors ; promoter regions ; Promoter Regions, Genetic - genetics ; recombinant DNA ; reporter genes ; structural genes ; Transformation, Genetic ; Vectors (cloning, transfer, expression). Insertion sequences and transposons ; White rot basidiomycete</subject><ispartof>Gene, 1994-05, Vol.142 (2), p.237-241</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-6f4c87980556ff0b16e324f142ae775b6b94d0d67b9fe89e21f4cc66b976ddb33</citedby><cites>FETCH-LOGICAL-c441t-6f4c87980556ff0b16e324f142ae775b6b94d0d67b9fe89e21f4cc66b976ddb33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(94)90267-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4089910$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8194757$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gessner, Matthias</creatorcontrib><creatorcontrib>Raeder, Ute</creatorcontrib><title>A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium</title><title>Gene</title><addtitle>Gene</addtitle><description>In this study, two transformation vectors (pMG101 and pMG103) for
Phanerochaete chrysosporium were constructed, based on the
ble phleomycin-resistance-encoding gene and a homologous histone
H4 promoter. Transformation frequencies were 6–10 per μg of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stably integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding
H3 gene in the promoter fragment, becomes methylated, resulting in inactivation of
ble-dependent resistance. Plasmid pMG103, which lacks the
H3, does not show methylation.</description><subject>antibiotics</subject><subject>Bacterial Proteins - genetics</subject><subject>Basidiomycota - drug effects</subject><subject>Basidiomycota - genetics</subject><subject>Basidiomycota - growth & development</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>ble gene</subject><subject>cytotoxic compounds</subject><subject>DNA transformation</subject><subject>DNA, Fungal - metabolism</subject><subject>drug resistance</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>filamentous fungus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic Markers - genetics</subject><subject>Genetic technics</subject><subject>genetic transformation</subject><subject>Genetic Vectors</subject><subject>histones</subject><subject>Histones - genetics</subject><subject>in vitro selection</subject><subject>Methods. Procedures. Technologies</subject><subject>Methylation</subject><subject>Phanerochaete chrysosporium</subject><subject>Phleomycins - pharmacology</subject><subject>plasmid vectors</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>recombinant DNA</subject><subject>reporter genes</subject><subject>structural genes</subject><subject>Transformation, Genetic</subject><subject>Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><subject>White rot basidiomycete</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKtvCPwDhA0JwCNiJY8eXSlVV2kqVigQ9W44zboySONjZiv33TNjVHsEXS57vPT2_IeQNZ5854_ILq1RTcM71Ry0-aVZKVYhnZMMbpQvGquY52RyRl-Q0558MT12XJ-Sk4VqoWm2Iu6B9yEucgN4IOqc4xgUS9TFR-D0nyDnEiUZPLZ37AeK4c2Eq8B1FdnJAHwGlYaLfejtBiq63sAB1fdrlmOeYwnZ8RV54O2R4fbjPyMPXqx-XN8Xd_fXt5cVd4YTgSyG9cJi9wYjSe9ZyCVUpPBelBaXqVrZadKyTqtUeGg0lR4GT-Kxk17VVdUY-7H3xG7-2kBczhuxgGDBZ3GajpGgaUfP_glyqSjG1Ooo96FLMOYE3cwqjTTvDmVmXYNaGzdqw0cL8XYIRKHt78N-2I3RH0aF1nL8_zG12dvAJmwz5iAnWaM0ZYu_2mLfR2MeEyMP3kvGKcYHzcjU63xOAtT4FSCa7ALiWLiRwi-li-HfSP3gRrLU</recordid><startdate>19940516</startdate><enddate>19940516</enddate><creator>Gessner, Matthias</creator><creator>Raeder, Ute</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19940516</creationdate><title>A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium</title><author>Gessner, Matthias ; Raeder, Ute</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-6f4c87980556ff0b16e324f142ae775b6b94d0d67b9fe89e21f4cc66b976ddb33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>antibiotics</topic><topic>Bacterial Proteins - genetics</topic><topic>Basidiomycota - drug effects</topic><topic>Basidiomycota - genetics</topic><topic>Basidiomycota - growth & development</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>ble gene</topic><topic>cytotoxic compounds</topic><topic>DNA transformation</topic><topic>DNA, Fungal - metabolism</topic><topic>drug resistance</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>filamentous fungus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic Markers - genetics</topic><topic>Genetic technics</topic><topic>genetic transformation</topic><topic>Genetic Vectors</topic><topic>histones</topic><topic>Histones - genetics</topic><topic>in vitro selection</topic><topic>Methods. Procedures. Technologies</topic><topic>Methylation</topic><topic>Phanerochaete chrysosporium</topic><topic>Phleomycins - pharmacology</topic><topic>plasmid vectors</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>recombinant DNA</topic><topic>reporter genes</topic><topic>structural genes</topic><topic>Transformation, Genetic</topic><topic>Vectors (cloning, transfer, expression). Insertion sequences and transposons</topic><topic>White rot basidiomycete</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gessner, Matthias</creatorcontrib><creatorcontrib>Raeder, Ute</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gessner, Matthias</au><au>Raeder, Ute</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1994-05-16</date><risdate>1994</risdate><volume>142</volume><issue>2</issue><spage>237</spage><epage>241</epage><pages>237-241</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>In this study, two transformation vectors (pMG101 and pMG103) for
Phanerochaete chrysosporium were constructed, based on the
ble phleomycin-resistance-encoding gene and a homologous histone
H4 promoter. Transformation frequencies were 6–10 per μg of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stably integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding
H3 gene in the promoter fragment, becomes methylated, resulting in inactivation of
ble-dependent resistance. Plasmid pMG103, which lacks the
H3, does not show methylation.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>8194757</pmid><doi>10.1016/0378-1119(94)90267-4</doi><tpages>5</tpages></addata></record> |
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| ispartof | Gene, 1994-05, Vol.142 (2), p.237-241 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | antibiotics Bacterial Proteins - genetics Basidiomycota - drug effects Basidiomycota - genetics Basidiomycota - growth & development Biological and medical sciences Biotechnology ble gene cytotoxic compounds DNA transformation DNA, Fungal - metabolism drug resistance Drug Resistance, Microbial - genetics filamentous fungus Fundamental and applied biological sciences. Psychology Genetic engineering Genetic Markers - genetics Genetic technics genetic transformation Genetic Vectors histones Histones - genetics in vitro selection Methods. Procedures. Technologies Methylation Phanerochaete chrysosporium Phleomycins - pharmacology plasmid vectors promoter regions Promoter Regions, Genetic - genetics recombinant DNA reporter genes structural genes Transformation, Genetic Vectors (cloning, transfer, expression). Insertion sequences and transposons White rot basidiomycete |
| title | A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium |
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