Cell free synthesis of Toxoplasma gondii antigens

Functionally active poly(A)-containing mRNA was isolated from tachyzoites of the RH strain of Toxoplasma gondii. The T. gondii mRNA was capable of directing the synthesis of proteins in a wheat germ in vitro translation system, but not in a cell free system derived from rabbit reticulocyte lysate. E...

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Veröffentlicht in:	Molecular and biochemical parasitology 1985-01, Vol.17 (2), p.163-170
Hauptverfasser:	Prince, Jeffrey B., Koven-Quinn, Marla A., Remington, Jack S., Sharma, Somesh D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals antigens Antigens - genetics Antigens - isolation & purification Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Cell-Free System Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Immunoprecipitation Kinetics messenger RNA Molecular Weight mRNA translation Plants - metabolism Poly A - genetics Protein Biosynthesis protein synthesis Protozoa Rabbits Reticulocytes - metabolism RNA, Messenger - genetics RNA, Messenger - isolation & purification Tachyzoite antigens Toxoplasma - genetics Toxoplasma gondii Triticum - metabolism
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