Reversed-phase high-performance liquid chromatography assay for recombinant acidic fibroblast growth factor in E. coli cell suspensions and lysate samples

A reversed-phase HPLC assay for acidic Fibroblast Growth Factor (aFGF) expressed in E. coli is described. The assay was developed on a Polymer Labs. PLRP-S macroporous poly(styrene-benzene) column. A sample preparation procedure was developed to permit quantitation of aFGF in crude samples, such as...

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Veröffentlicht in:	Journal of Chromatography A 1994-03, Vol.663 (1), p.43-51
Hauptverfasser:	DePhillips, P., Buckland, B., Gbewonyo, K., Yamazaki, S., Sitrin, R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, High Pressure Liquid - methods Escherichia coli - genetics Fermentation Fibroblast Growth Factor 1 - analysis Fibroblast Growth Factor 1 - genetics Recombinant Proteins - analysis Recombinant Proteins - genetics Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet
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