Reversed-phase high-performance liquid chromatography assay for recombinant acidic fibroblast growth factor in E. coli cell suspensions and lysate samples
A reversed-phase HPLC assay for acidic Fibroblast Growth Factor (aFGF) expressed in E. coli is described. The assay was developed on a Polymer Labs. PLRP-S macroporous poly(styrene-benzene) column. A sample preparation procedure was developed to permit quantitation of aFGF in crude samples, such as...
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| Veröffentlicht in: | Journal of Chromatography A 1994-03, Vol.663 (1), p.43-51 |
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| container_title | Journal of Chromatography A |
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| creator | DePhillips, P. Buckland, B. Gbewonyo, K. Yamazaki, S. Sitrin, R. |
| description | A reversed-phase HPLC assay for acidic Fibroblast Growth Factor (aFGF) expressed in
E. coli is described. The assay was developed on a Polymer Labs. PLRP-S macroporous poly(styrene-benzene) column. A sample preparation procedure was developed to permit quantitation of aFGF in crude samples, such as cell suspensions and cell lysates. The assay was linear over the range of 25–140 μg/ml with an accuracy of 5%. By using a POROS column containing a stationary phase with “through-pores” and a superficial mobile phase velocity of 1440 cm/h, the analysis could be performed in 3 min. The polymeric supports used for this assay were durable; periodic washing with sodium hydroxide maintained column performance for extended periods. |
| doi_str_mv | 10.1016/0021-9673(94)80494-X |
| format | Article |
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E. coli is described. The assay was developed on a Polymer Labs. PLRP-S macroporous poly(styrene-benzene) column. A sample preparation procedure was developed to permit quantitation of aFGF in crude samples, such as cell suspensions and cell lysates. The assay was linear over the range of 25–140 μg/ml with an accuracy of 5%. By using a POROS column containing a stationary phase with “through-pores” and a superficial mobile phase velocity of 1440 cm/h, the analysis could be performed in 3 min. The polymeric supports used for this assay were durable; periodic washing with sodium hydroxide maintained column performance for extended periods.</description><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Escherichia coli - genetics</subject><subject>Fermentation</subject><subject>Fibroblast Growth Factor 1 - analysis</subject><subject>Fibroblast Growth Factor 1 - genetics</subject><subject>Recombinant Proteins - analysis</subject><subject>Recombinant Proteins - genetics</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1v1TAQzAFUSuEfgOQTgkOKHTt2ckFCVWmRKiEhkHqzNs7mxSiJU69T9P4KvxY_3lOPnFbamdmPmaJ4I_il4EJ_5LwSZauNfN-qDw1XrSrvnxXnT-0XxUuiX5wLw011VpyZWiiu9Xnx5zs-YiTsy3UEQjb63ViuGIcQZ1gcssk_bL5nboxhhhR2EdZxz4AI9iyTWEQX5s4vsCQGzvfescF3MXQTUGK7GH6nkQ3gUub6hV1fMhcmzxxOE6ONVlzIh4UYLD2b9gQJGcG8TkiviucDTISvT_Wi-Pnl-sfVbXn37ebr1ee70lWmSmVdCail0Ro1SCM6hRJarpxqalS6FR3IppdOVYBCGSebIcuAu4zpqmsHeVG8O85dY3jYkJKdPR3ugwXDRtZo1RhZV5mojkQXA1HEwa7RzxD3VnB7iMEe_LYHv22r7L8Y7H2WvT3N37oZ-yfRKYOMfzrimJ989BgtOY_Z-95nc5Ptg___gr9Cz5x7</recordid><startdate>19940304</startdate><enddate>19940304</enddate><creator>DePhillips, P.</creator><creator>Buckland, B.</creator><creator>Gbewonyo, K.</creator><creator>Yamazaki, S.</creator><creator>Sitrin, R.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940304</creationdate><title>Reversed-phase high-performance liquid chromatography assay for recombinant acidic fibroblast growth factor in E. coli cell suspensions and lysate samples</title><author>DePhillips, P. ; Buckland, B. ; Gbewonyo, K. ; Yamazaki, S. ; Sitrin, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-521a53766e6a371b4e3a904c485e4691ba38d3c42ae147c38f272a0c46962b9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Escherichia coli - genetics</topic><topic>Fermentation</topic><topic>Fibroblast Growth Factor 1 - analysis</topic><topic>Fibroblast Growth Factor 1 - genetics</topic><topic>Recombinant Proteins - analysis</topic><topic>Recombinant Proteins - genetics</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DePhillips, P.</creatorcontrib><creatorcontrib>Buckland, B.</creatorcontrib><creatorcontrib>Gbewonyo, K.</creatorcontrib><creatorcontrib>Yamazaki, S.</creatorcontrib><creatorcontrib>Sitrin, R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DePhillips, P.</au><au>Buckland, B.</au><au>Gbewonyo, K.</au><au>Yamazaki, S.</au><au>Sitrin, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reversed-phase high-performance liquid chromatography assay for recombinant acidic fibroblast growth factor in E. coli cell suspensions and lysate samples</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>1994-03-04</date><risdate>1994</risdate><volume>663</volume><issue>1</issue><spage>43</spage><epage>51</epage><pages>43-51</pages><issn>0021-9673</issn><abstract>A reversed-phase HPLC assay for acidic Fibroblast Growth Factor (aFGF) expressed in
E. coli is described. The assay was developed on a Polymer Labs. PLRP-S macroporous poly(styrene-benzene) column. A sample preparation procedure was developed to permit quantitation of aFGF in crude samples, such as cell suspensions and cell lysates. The assay was linear over the range of 25–140 μg/ml with an accuracy of 5%. By using a POROS column containing a stationary phase with “through-pores” and a superficial mobile phase velocity of 1440 cm/h, the analysis could be performed in 3 min. The polymeric supports used for this assay were durable; periodic washing with sodium hydroxide maintained column performance for extended periods.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7514066</pmid><doi>10.1016/0021-9673(94)80494-X</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of Chromatography A, 1994-03, Vol.663 (1), p.43-51 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Chromatography, High Pressure Liquid - methods Escherichia coli - genetics Fermentation Fibroblast Growth Factor 1 - analysis Fibroblast Growth Factor 1 - genetics Recombinant Proteins - analysis Recombinant Proteins - genetics Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet |
| title | Reversed-phase high-performance liquid chromatography assay for recombinant acidic fibroblast growth factor in E. coli cell suspensions and lysate samples |
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