A Physical Map of the Region Encompassing the Human Interleukin-1α, Interleukin-1β, and Interleukin-1 Receptor Antagonist Genes

We have constructed a restriction map of the human genomic region containing the genes encoding the three members of the interleukin-1 (IL-1) family, IL-1α, IL-1β, and IL-1 receptor antagonist (IL-1ra). For this purpose, pulsed-field gel electrophoresis blots were hybridized with probes derived from...

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Veröffentlicht in:Genomics (San Diego, Calif.) Calif.), 1994-01, Vol.19 (2), p.382-384
Hauptverfasser: Nicklin, Martin J.H., Weith, Andreas, Duff, Gordon W.
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Sprache:eng
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Zusammenfassung:We have constructed a restriction map of the human genomic region containing the genes encoding the three members of the interleukin-1 (IL-1) family, IL-1α, IL-1β, and IL-1 receptor antagonist (IL-1ra). For this purpose, pulsed-field gel electrophoresis blots were hybridized with probes derived from the three genes. The genes (IL1A, IL1B, and IL1RN, respectively) were found to map to a common restriction fragment of approximately 430 kb that is flanked by two clusters of sites for methylation-sensitive rare-cutter restriction enzymes (putative CpG islands). A likely third internal CpG island was marked by two rare-cutter sites. CpG and non-CpG-specific enzymes were used to map the three genes. Relative to one terminal CpG island, the three genes were mapped to the following intervals: IL1A was between +0 and +35 kb, IL1B was between +70 and +110 kb, and IL1RN was between 1330 and +430 kb.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1994.1076