Dexamethasone-Mediated Induction of MMTV- myf5 in DD3 Myoblasts Increases Endogenous myogenin Expression but Does Not Transactivate myf5

DD3 cells are a myoblast line generated by stable transfection of C3H10T1/2 fibroblasts with the bovine myf5 cDNA ( bmyf) fused to the dexamethasone-inducible MMTV promoter. After treating proliferating cells with dexamethasone, bmyf transcripts were induced approximately fivefold within 1.5-2.5 h....

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Veröffentlicht in:	Experimental cell research 1994-06, Vol.212 (2), p.321-328
Hauptverfasser:	Arnold, Todd E., Worrell, Robert A., Barth, Jeremy L., Morris, Julie, Ivarie, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cattle Dexamethasone - pharmacology DNA-Binding Proteins - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genes Genes. Genome In Vitro Techniques MEF2 Transcription Factors Mice Molecular and cellular biology Molecular genetics Muscle Proteins - genetics Muscle Proteins - physiology Myogenic Regulatory Factor 5 Myogenic Regulatory Factors - metabolism Myogenin - genetics Oligodeoxyribonucleotides - chemistry RNA, Messenger - genetics Trans-Activators Transcription Factors - metabolism Transfection
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