N-terminal variants of fatty acid-binding protein from bovine heart overexpressed in Escherichia coli

An expression vector for bovine heart fatty acid-binding protein (H-FABP) was constructed by introducing the coding part of the cDNA into the pET-3d vector. Transformed Escherichia coli strain BL21 (DE3)pLysS produced functional recombinant H-FABP up to 40% of the soluble proteins. The expression of...

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Veröffentlicht in:	Journal of biotechnology 1994-04, Vol.33 (3), p.259-269
Hauptverfasser:	Specht, Bernfried, Oudenampsen-Krüger, Elke, Ingendoh, Arnd, Hillenkamp, Franz, Lezius, Axel G., Spener, Friedrich
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Biotechnology Carrier Proteins - biosynthesis Carrier Proteins - chemistry Carrier Proteins - genetics Cattle Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Fatty acid-binding protein Fatty Acid-Binding Proteins Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics MALDI mass spectrometry Mass Spectrometry Methods. Procedures. Technologies Modification of gene expression level Molecular Sequence Data Neoplasm Proteins Overexpression Protein heterogeneity Recombinant Proteins - biosynthesis Variant
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