Specific binding of [ 3H]heparin to bovine granulosa cell membranes

Bovine granulosa cell membranes from small (SFM) and large (LFM) antral follicles were incubated with [ 3H]heparin, a commercial radioactively labeled glycosaminoglycan (GAG). Binding was specific, reversible, saturable, and dependent on time, pH, ionic strength and divalent cations. SFM exhibited d...

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Veröffentlicht in:	Molecular and cellular endocrinology 1985-09, Vol.42 (2), p.135-144
Hauptverfasser:	Bushmeyer, S.M., Bellin, M.E., Ax, R.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals binding Biological and medical sciences Calcium - pharmacology Cattle Cell Membrane - metabolism Female follicle Fundamental and applied biological sciences. Psychology glycosaminoglycans granulosa cells Granulosa Cells - metabolism heparin Heparin - metabolism Hydrogen-Ion Concentration Kinetics Magnesium - pharmacology Mammalian female genital system Morphology. Physiology Ovarian Follicle - anatomy & histology Sodium Chloride - pharmacology Vertebrates: reproduction
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