Purification and characterization of an endogenous inhibitor specific to the Z-Leu-Leu-Leu-MCA degrading activity in proteasome and its identification as heat-shock protein 90
We previously identified a benzyloxycarbonyl(Z)-Leu-Leu-Leu-4-methylcoumaryl-7-amide (ZLLL-MCA) degrading activity in proteasome as a candidate for the regulator of neurite outgrowth. As its counterpart, we purified a protein from bovine brain that specifically inhibits the ZLLL-MCA degrading activi...
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| Veröffentlicht in: | FEBS letters 1994-05, Vol.344 (2), p.229-233 |
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| description | We previously identified a benzyloxycarbonyl(Z)-Leu-Leu-Leu-4-methylcoumaryl-7-amide (ZLLL-MCA) degrading activity in proteasome as a candidate for the regulator of neurite outgrowth. As its counterpart, we purified a protein from bovine brain that specifically inhibits the ZLLL-MCA degrading activity in proteasome. This protein is heat stable and has no effect on the other catalytic activities in proteasome, or on the activities of trypsin, chymotrypsin, or m- and μ-calpains either. The molar ratio of inhibitor-to-proteasome that inhibits 50% of the ZLLL-MCA degrading activity of proteasome is 1:1. The inhibitory mechanism of the inhibitor against proteasome is non-competitive. Finally, the inhibitor was identified as heat-shock protein 90 (HSP90) by partial amino acid sequencing and immunodetection. The results suggest that HSP90 initiates neurite outgrowth through the inhibition of the ZLLL-MCA degrading activity in proteasome. |
| doi_str_mv | 10.1016/0014-5793(94)00388-2 |
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As its counterpart, we purified a protein from bovine brain that specifically inhibits the ZLLL-MCA degrading activity in proteasome. This protein is heat stable and has no effect on the other catalytic activities in proteasome, or on the activities of trypsin, chymotrypsin, or m- and μ-calpains either. The molar ratio of inhibitor-to-proteasome that inhibits 50% of the ZLLL-MCA degrading activity of proteasome is 1:1. The inhibitory mechanism of the inhibitor against proteasome is non-competitive. Finally, the inhibitor was identified as heat-shock protein 90 (HSP90) by partial amino acid sequencing and immunodetection. The results suggest that HSP90 initiates neurite outgrowth through the inhibition of the ZLLL-MCA degrading activity in proteasome.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(94)00388-2</identifier><identifier>PMID: 8187890</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Boc-VLK-MCA, tertiary butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide ; Brain - enzymology ; buffer A, 20 mM Tris-HCl, pH 7.0, containing 10% glycerol ; buffer B, 20 mM Tris-HCl, pH 7.5, containing 5 mM EDTA and 10% glycerol ; Cattle ; Electrophoresis, Polyacrylamide Gel ; Endopeptidases - metabolism ; Fluorescent Dyes ; Heat-shock protein 90 ; Heat-Shock Proteins - chemistry ; Heat-Shock Proteins - isolation & purification ; HSP, heat-shock protein ; MCA, 4-methylcoumaryl-7-amide ; Molecular Sequence Data ; Molecular Weight ; Neurite outgrowth ; Oligopeptides - metabolism ; PAGE, polyacrylamide gel electrophoresis ; Protease Inhibitors - chemistry ; Protease Inhibitors - isolation & purification ; Protease Inhibitors - pharmacology ; Proteasome ; Proteasome inhibitor ; PVDF, polyvinylidene difluoride ; Sequence Analysis ; SucLLVY-MCA, succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide ; Z, benzyloxycarbonyl ; ZLLE-NA, benzyloxycarbonyl-Leu-Leu-Glu-β-naphthyl-amide ; ZLLL-MCA, benzyloxycarbonyl-Leu-Leu-4-methylcoumaryl-7-amide ; ZLLLal, benzyloxycarbonyl-Leu-Leu-leucinal</subject><ispartof>FEBS letters, 1994-05, Vol.344 (2), p.229-233</ispartof><rights>1994</rights><rights>FEBS Letters 344 (1994) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5322-f8b2d0c9716bfd48a586dcb7aff576bba0e7ab79c86b75c174798535d85f349d3</citedby><cites>FETCH-LOGICAL-c5322-f8b2d0c9716bfd48a586dcb7aff576bba0e7ab79c86b75c174798535d85f349d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579394003882$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8187890$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsubuki, Satoshi</creatorcontrib><creatorcontrib>Saito, Yumiko</creatorcontrib><creatorcontrib>Kawashima, Seiichi</creatorcontrib><title>Purification and characterization of an endogenous inhibitor specific to the Z-Leu-Leu-Leu-MCA degrading activity in proteasome and its identification as heat-shock protein 90</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We previously identified a benzyloxycarbonyl(Z)-Leu-Leu-Leu-4-methylcoumaryl-7-amide (ZLLL-MCA) degrading activity in proteasome as a candidate for the regulator of neurite outgrowth. As its counterpart, we purified a protein from bovine brain that specifically inhibits the ZLLL-MCA degrading activity in proteasome. This protein is heat stable and has no effect on the other catalytic activities in proteasome, or on the activities of trypsin, chymotrypsin, or m- and μ-calpains either. The molar ratio of inhibitor-to-proteasome that inhibits 50% of the ZLLL-MCA degrading activity of proteasome is 1:1. The inhibitory mechanism of the inhibitor against proteasome is non-competitive. Finally, the inhibitor was identified as heat-shock protein 90 (HSP90) by partial amino acid sequencing and immunodetection. The results suggest that HSP90 initiates neurite outgrowth through the inhibition of the ZLLL-MCA degrading activity in proteasome.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Boc-VLK-MCA, tertiary butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide</subject><subject>Brain - enzymology</subject><subject>buffer A, 20 mM Tris-HCl, pH 7.0, containing 10% glycerol</subject><subject>buffer B, 20 mM Tris-HCl, pH 7.5, containing 5 mM EDTA and 10% glycerol</subject><subject>Cattle</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Endopeptidases - metabolism</subject><subject>Fluorescent Dyes</subject><subject>Heat-shock protein 90</subject><subject>Heat-Shock Proteins - chemistry</subject><subject>Heat-Shock Proteins - isolation & purification</subject><subject>HSP, heat-shock protein</subject><subject>MCA, 4-methylcoumaryl-7-amide</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Neurite outgrowth</subject><subject>Oligopeptides - metabolism</subject><subject>PAGE, polyacrylamide gel electrophoresis</subject><subject>Protease Inhibitors - chemistry</subject><subject>Protease Inhibitors - isolation & purification</subject><subject>Protease Inhibitors - pharmacology</subject><subject>Proteasome</subject><subject>Proteasome inhibitor</subject><subject>PVDF, polyvinylidene difluoride</subject><subject>Sequence Analysis</subject><subject>SucLLVY-MCA, succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide</subject><subject>Z, benzyloxycarbonyl</subject><subject>ZLLE-NA, benzyloxycarbonyl-Leu-Leu-Glu-β-naphthyl-amide</subject><subject>ZLLL-MCA, benzyloxycarbonyl-Leu-Leu-4-methylcoumaryl-7-amide</subject><subject>ZLLLal, benzyloxycarbonyl-Leu-Leu-leucinal</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUcGO0zAQtRBo6S78AUg-ITgE7MSJ7QvSUm0BqQgOcOFiOfakMbRx13YWlZ_aX8RpqooT4mCN_Oa9N6N5CD2j5DUltHlDCGVFzWX1UrJXhFRCFOUDtKCCV0XFGvEQLc6Ux-gyxh8k_wWVF-hCZJaQZIHuv4zBdc7o5PyA9WCx6XXQJkFwv2fQdxnHMFi_gcGPEbuhd61LPuC4BzOpcfI49YC_F2sYz-_T8hpb2ARt3bDB2dPduXTIcrwPPoGOfgfHkS5lUwtD-muTiHvQqYi9Nz9nftZJ8gQ96vQ2wtNTvULfVjdflx-K9ef3H5fX68LUVVkWnWhLS4zktGk7y4SuRWNNy3XX1bxpW02A65ZLI5qW14ZyxqWoq9qKuquYtNUVejH75tG3I8Skdi4a2G71APkGijdMlA1hmchmogk-xgCd2ge30-GgKFFTTmoKQU0hKMnUMSdVZtnzk__Y7sCeRadgcn8193-5LRz-y1Otbt6VU2PCJTui06C3sxHka905CCoaB4MB6wKYpKx3_970D1RkuHo</recordid><startdate>19940516</startdate><enddate>19940516</enddate><creator>Tsubuki, Satoshi</creator><creator>Saito, Yumiko</creator><creator>Kawashima, Seiichi</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940516</creationdate><title>Purification and characterization of an endogenous inhibitor specific to the Z-Leu-Leu-Leu-MCA degrading activity in proteasome and its identification as heat-shock protein 90</title><author>Tsubuki, Satoshi ; Saito, Yumiko ; Kawashima, Seiichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5322-f8b2d0c9716bfd48a586dcb7aff576bba0e7ab79c86b75c174798535d85f349d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Boc-VLK-MCA, tertiary butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide</topic><topic>Brain - enzymology</topic><topic>buffer A, 20 mM Tris-HCl, pH 7.0, containing 10% glycerol</topic><topic>buffer B, 20 mM Tris-HCl, pH 7.5, containing 5 mM EDTA and 10% glycerol</topic><topic>Cattle</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Endopeptidases - metabolism</topic><topic>Fluorescent Dyes</topic><topic>Heat-shock protein 90</topic><topic>Heat-Shock Proteins - chemistry</topic><topic>Heat-Shock Proteins - isolation & purification</topic><topic>HSP, heat-shock protein</topic><topic>MCA, 4-methylcoumaryl-7-amide</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>Neurite outgrowth</topic><topic>Oligopeptides - metabolism</topic><topic>PAGE, polyacrylamide gel electrophoresis</topic><topic>Protease Inhibitors - chemistry</topic><topic>Protease Inhibitors - isolation & purification</topic><topic>Protease Inhibitors - pharmacology</topic><topic>Proteasome</topic><topic>Proteasome inhibitor</topic><topic>PVDF, polyvinylidene difluoride</topic><topic>Sequence Analysis</topic><topic>SucLLVY-MCA, succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide</topic><topic>Z, benzyloxycarbonyl</topic><topic>ZLLE-NA, benzyloxycarbonyl-Leu-Leu-Glu-β-naphthyl-amide</topic><topic>ZLLL-MCA, benzyloxycarbonyl-Leu-Leu-4-methylcoumaryl-7-amide</topic><topic>ZLLLal, benzyloxycarbonyl-Leu-Leu-leucinal</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsubuki, Satoshi</creatorcontrib><creatorcontrib>Saito, Yumiko</creatorcontrib><creatorcontrib>Kawashima, Seiichi</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsubuki, Satoshi</au><au>Saito, Yumiko</au><au>Kawashima, Seiichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and characterization of an endogenous inhibitor specific to the Z-Leu-Leu-Leu-MCA degrading activity in proteasome and its identification as heat-shock protein 90</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1994-05-16</date><risdate>1994</risdate><volume>344</volume><issue>2</issue><spage>229</spage><epage>233</epage><pages>229-233</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>We previously identified a benzyloxycarbonyl(Z)-Leu-Leu-Leu-4-methylcoumaryl-7-amide (ZLLL-MCA) degrading activity in proteasome as a candidate for the regulator of neurite outgrowth. As its counterpart, we purified a protein from bovine brain that specifically inhibits the ZLLL-MCA degrading activity in proteasome. This protein is heat stable and has no effect on the other catalytic activities in proteasome, or on the activities of trypsin, chymotrypsin, or m- and μ-calpains either. The molar ratio of inhibitor-to-proteasome that inhibits 50% of the ZLLL-MCA degrading activity of proteasome is 1:1. The inhibitory mechanism of the inhibitor against proteasome is non-competitive. Finally, the inhibitor was identified as heat-shock protein 90 (HSP90) by partial amino acid sequencing and immunodetection. The results suggest that HSP90 initiates neurite outgrowth through the inhibition of the ZLLL-MCA degrading activity in proteasome.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>8187890</pmid><doi>10.1016/0014-5793(94)00388-2</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1994-05, Vol.344 (2), p.229-233 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Boc-VLK-MCA, tertiary butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide Brain - enzymology buffer A, 20 mM Tris-HCl, pH 7.0, containing 10% glycerol buffer B, 20 mM Tris-HCl, pH 7.5, containing 5 mM EDTA and 10% glycerol Cattle Electrophoresis, Polyacrylamide Gel Endopeptidases - metabolism Fluorescent Dyes Heat-shock protein 90 Heat-Shock Proteins - chemistry Heat-Shock Proteins - isolation & purification HSP, heat-shock protein MCA, 4-methylcoumaryl-7-amide Molecular Sequence Data Molecular Weight Neurite outgrowth Oligopeptides - metabolism PAGE, polyacrylamide gel electrophoresis Protease Inhibitors - chemistry Protease Inhibitors - isolation & purification Protease Inhibitors - pharmacology Proteasome Proteasome inhibitor PVDF, polyvinylidene difluoride Sequence Analysis SucLLVY-MCA, succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide Z, benzyloxycarbonyl ZLLE-NA, benzyloxycarbonyl-Leu-Leu-Glu-β-naphthyl-amide ZLLL-MCA, benzyloxycarbonyl-Leu-Leu-4-methylcoumaryl-7-amide ZLLLal, benzyloxycarbonyl-Leu-Leu-leucinal |
| title | Purification and characterization of an endogenous inhibitor specific to the Z-Leu-Leu-Leu-MCA degrading activity in proteasome and its identification as heat-shock protein 90 |
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