Effect of culture conditions on the NADH/NAD ratio and total amounts of NAD(H) in chemostat cultures of Enterococcus faecalis NCTC 775
Enterococcus faecalis was grown in chemostat culture on various energy sources at dilution rates ranging from 0.05 h−1 to 0.5 h−1, under both aerobic and anaerobic conditions. NADH/NAD ratios and total nicotinamide adenine dinucleotide pool size (NAD(H)) were determined. It was found that the NADH/N...
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| Veröffentlicht in: | FEMS microbiology letters 1994-03, Vol.116 (3), p.263-267 |
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| creator | Snoep, Jacky L. Graef, Mark R. Mattos, M.Joost Teixeira Neijssel, Oense M. |
| description | Enterococcus faecalis was grown in chemostat culture on various energy sources at dilution rates ranging from 0.05 h−1 to 0.5 h−1, under both aerobic and anaerobic conditions. NADH/NAD ratios and total nicotinamide adenine dinucleotide pool size (NAD(H)) were determined. It was found that the NADH/NAD ratio was controlled by the steady state product concentrations rather than by the degree of reduction of the energy source. Highest ratios were observed when NADH was reoxidized via ethanol formation, whereas in aerobic cultures, in which predominantly acetate was produced and oxidation of NADH occurred via the NADH oxidase, ratios were lowest. Addition of ethanol to the medium resulted in an increase of the NADH/NAD ratio, both aerobically and anaerobically. The total amount of NAD(H) was found to be influenced by the culture conditions. Under anaerobic conditions, the NADH oxidation (NAD reduction) rate appeared to correlate with the total amount of nicotinamide nucleotides. In contrast, no effect of the culture conditions on the total amount of NAD(H) was observed in aerobically grown cells. |
| doi_str_mv | 10.1111/j.1574-6968.1994.tb06713.x |
| format | Article |
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NADH/NAD ratios and total nicotinamide adenine dinucleotide pool size (NAD(H)) were determined. It was found that the NADH/NAD ratio was controlled by the steady state product concentrations rather than by the degree of reduction of the energy source. Highest ratios were observed when NADH was reoxidized via ethanol formation, whereas in aerobic cultures, in which predominantly acetate was produced and oxidation of NADH occurred via the NADH oxidase, ratios were lowest. Addition of ethanol to the medium resulted in an increase of the NADH/NAD ratio, both aerobically and anaerobically. The total amount of NAD(H) was found to be influenced by the culture conditions. Under anaerobic conditions, the NADH oxidation (NAD reduction) rate appeared to correlate with the total amount of nicotinamide nucleotides. In contrast, no effect of the culture conditions on the total amount of NAD(H) was observed in aerobically grown cells.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1994.tb06713.x</identifier><identifier>PMID: 8181697</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acetates - metabolism ; Acetic Acid ; Aerobiosis ; Anaerobiosis ; Bacteriological Techniques ; Bacteriology ; Biological and medical sciences ; Chemostat culture ; Enterococcus faecalis ; Enterococcus faecalis - growth & development ; Enterococcus faecalis - metabolism ; Ethanol - metabolism ; Fundamental and applied biological sciences. Psychology ; Gluconates - metabolism ; Glucose - metabolism ; Lactates - metabolism ; Lactic Acid ; Metabolism. 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NADH/NAD ratios and total nicotinamide adenine dinucleotide pool size (NAD(H)) were determined. It was found that the NADH/NAD ratio was controlled by the steady state product concentrations rather than by the degree of reduction of the energy source. Highest ratios were observed when NADH was reoxidized via ethanol formation, whereas in aerobic cultures, in which predominantly acetate was produced and oxidation of NADH occurred via the NADH oxidase, ratios were lowest. Addition of ethanol to the medium resulted in an increase of the NADH/NAD ratio, both aerobically and anaerobically. The total amount of NAD(H) was found to be influenced by the culture conditions. Under anaerobic conditions, the NADH oxidation (NAD reduction) rate appeared to correlate with the total amount of nicotinamide nucleotides. In contrast, no effect of the culture conditions on the total amount of NAD(H) was observed in aerobically grown cells.</description><subject>Acetates - metabolism</subject><subject>Acetic Acid</subject><subject>Aerobiosis</subject><subject>Anaerobiosis</subject><subject>Bacteriological Techniques</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chemostat culture</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecalis - growth & development</subject><subject>Enterococcus faecalis - metabolism</subject><subject>Ethanol - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gluconates - metabolism</subject><subject>Glucose - metabolism</subject><subject>Lactates - metabolism</subject><subject>Lactic Acid</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>NAD - metabolism</subject><subject>NADH/NAD ratio</subject><subject>Oxidation-Reduction</subject><subject>Redox potential</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkc1u1DAURi0EKkPhEZAshBAsktrxX9wFUjVMGaShbMra8jjXakZJXGJHtC_Ac-MwYbYIL2zJ37nX1j0IvaGkpHldHEoqFC-klnVJteZl2hOpKCsfnqDVKXqKVoSpuqBEq-foRYwHQgiviDxDZzWtqdRqhX5tvAeXcPDYTV2aRsAuDE2b2jBEHAac7gDfXH3aXuQNjzbfYzs0OIVkO2z7MA0pztU5fr_9gNsBuzvoQ0w2_e34J98MCcbggnNTxN6Cs10b8c36do2VEi_RM2-7CK-W8xx9v97crrfF7tvnL-urXeF4pVlR1dRp2ENTgXBeWCtYrfaUK0eqGqTVqgLqqbNeMiYBGiAatCCNkpoSDuwcvTv2vR_DjwliMn0bHXSdHSBM0SjJVV1x8k-QSskEYzSDl0fQjSHGEby5H9vejo-GEjPbMgczKzGzEjPbMost85CLXy-vTPsemlPpoifnb5fcxjwwP9rBtfGE8exT1CJjH4_Yz7aDx__4gLn-uqvyqH4DIzqwwA</recordid><startdate>199403</startdate><enddate>199403</enddate><creator>Snoep, Jacky L.</creator><creator>Graef, Mark R.</creator><creator>Mattos, M.Joost Teixeira</creator><creator>Neijssel, Oense M.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>199403</creationdate><title>Effect of culture conditions on the NADH/NAD ratio and total amounts of NAD(H) in chemostat cultures of Enterococcus faecalis NCTC 775</title><author>Snoep, Jacky L. ; Graef, Mark R. ; Mattos, M.Joost Teixeira ; Neijssel, Oense M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4293-281c9ebed2e5cf5aa5387b147c028e6a972e1f1caf6336eede09e950d769104e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Acetates - metabolism</topic><topic>Acetic Acid</topic><topic>Aerobiosis</topic><topic>Anaerobiosis</topic><topic>Bacteriological Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chemostat culture</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecalis - growth & development</topic><topic>Enterococcus faecalis - metabolism</topic><topic>Ethanol - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gluconates - metabolism</topic><topic>Glucose - metabolism</topic><topic>Lactates - metabolism</topic><topic>Lactic Acid</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>NAD - metabolism</topic><topic>NADH/NAD ratio</topic><topic>Oxidation-Reduction</topic><topic>Redox potential</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Snoep, Jacky L.</creatorcontrib><creatorcontrib>Graef, Mark R.</creatorcontrib><creatorcontrib>Mattos, M.Joost Teixeira</creatorcontrib><creatorcontrib>Neijssel, Oense M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Snoep, Jacky L.</au><au>Graef, Mark R.</au><au>Mattos, M.Joost Teixeira</au><au>Neijssel, Oense M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of culture conditions on the NADH/NAD ratio and total amounts of NAD(H) in chemostat cultures of Enterococcus faecalis NCTC 775</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1994-03</date><risdate>1994</risdate><volume>116</volume><issue>3</issue><spage>263</spage><epage>267</epage><pages>263-267</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Enterococcus faecalis was grown in chemostat culture on various energy sources at dilution rates ranging from 0.05 h−1 to 0.5 h−1, under both aerobic and anaerobic conditions. NADH/NAD ratios and total nicotinamide adenine dinucleotide pool size (NAD(H)) were determined. It was found that the NADH/NAD ratio was controlled by the steady state product concentrations rather than by the degree of reduction of the energy source. Highest ratios were observed when NADH was reoxidized via ethanol formation, whereas in aerobic cultures, in which predominantly acetate was produced and oxidation of NADH occurred via the NADH oxidase, ratios were lowest. Addition of ethanol to the medium resulted in an increase of the NADH/NAD ratio, both aerobically and anaerobically. The total amount of NAD(H) was found to be influenced by the culture conditions. Under anaerobic conditions, the NADH oxidation (NAD reduction) rate appeared to correlate with the total amount of nicotinamide nucleotides. In contrast, no effect of the culture conditions on the total amount of NAD(H) was observed in aerobically grown cells.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8181697</pmid><doi>10.1111/j.1574-6968.1994.tb06713.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | FEMS microbiology letters, 1994-03, Vol.116 (3), p.263-267 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | Oxford University Press Journals Digital Archive legacy; MEDLINE; Access via Wiley Online Library; Alma/SFX Local Collection |
| subjects | Acetates - metabolism Acetic Acid Aerobiosis Anaerobiosis Bacteriological Techniques Bacteriology Biological and medical sciences Chemostat culture Enterococcus faecalis Enterococcus faecalis - growth & development Enterococcus faecalis - metabolism Ethanol - metabolism Fundamental and applied biological sciences. Psychology Gluconates - metabolism Glucose - metabolism Lactates - metabolism Lactic Acid Metabolism. Enzymes Microbiology NAD - metabolism NADH/NAD ratio Oxidation-Reduction Redox potential |
| title | Effect of culture conditions on the NADH/NAD ratio and total amounts of NAD(H) in chemostat cultures of Enterococcus faecalis NCTC 775 |
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