Bacteria identification of otitis media with fluorescence spectroscopy
We have investigated the fluorescence profiles of four common pathogens: S. pneumoniae, S. aureus, M. catarrhalis, and H. influenzae. The steady‐state auto fluorescence spectra of bacteria are measured as a function of the incident light from 200 to 700 nm. The spectra for each bacterium are combine...
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| Veröffentlicht in: | Lasers in surgery and medicine 1994, Vol.14 (2), p.155-163 |
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| container_title | Lasers in surgery and medicine |
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| creator | Sorrell, Matthew J. Tribble, Jerri Reinisch, Lou Werkhaven, Jay A. Ossoff, Robert H. |
| description | We have investigated the fluorescence profiles of four common pathogens: S. pneumoniae, S. aureus, M. catarrhalis, and H. influenzae. The steady‐state auto fluorescence spectra of bacteria are measured as a function of the incident light from 200 to 700 nm. The spectra for each bacterium are combined into a fluorescence profile or fluorescence finger print. Each bacterium produces a unique in vitro fluorescence profile when measured in a saline suspension. The profiles are reproducible. Suspensions of a bacterial strain, where the identification is not known, can be correctly matched to a small library of previously measured fluorescence profiles using a linear least‐squares fitting algorithm. In addition, we have measured the fluorescence and absorption spectrum of the tympanic membrane removed from a chinchilla. The optical properties of the tympanic membrane and the least‐squares identification process form precept for a non‐invasive, fluorescence based bacterial diagnosis technique to be used in otitis media. © 1994 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/1096-9101(1994)14:2<155::AID-LSM1900140208>3.0.CO;2-L |
| format | Article |
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The steady‐state auto fluorescence spectra of bacteria are measured as a function of the incident light from 200 to 700 nm. The spectra for each bacterium are combined into a fluorescence profile or fluorescence finger print. Each bacterium produces a unique in vitro fluorescence profile when measured in a saline suspension. The profiles are reproducible. Suspensions of a bacterial strain, where the identification is not known, can be correctly matched to a small library of previously measured fluorescence profiles using a linear least‐squares fitting algorithm. In addition, we have measured the fluorescence and absorption spectrum of the tympanic membrane removed from a chinchilla. The optical properties of the tympanic membrane and the least‐squares identification process form precept for a non‐invasive, fluorescence based bacterial diagnosis technique to be used in otitis media. © 1994 Wiley‐Liss, Inc.</description><identifier>ISSN: 0196-8092</identifier><identifier>EISSN: 1096-9101</identifier><identifier>DOI: 10.1002/1096-9101(1994)14:2<155::AID-LSM1900140208>3.0.CO;2-L</identifier><identifier>PMID: 8183049</identifier><identifier>CODEN: LSMEDI</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>acute otitis media ; Algorithms ; Animals ; bacteria identification ; Biological and medical sciences ; Chinchilla ; Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology ; fluorescence ; Haemophilus influenzae - isolation & purification ; Humans ; Least-Squares Analysis ; Medical sciences ; Moraxella (Branhamella) catarrhalis - isolation & purification ; Non tumoral diseases ; noninvasive diagnostics ; Otitis Media - microbiology ; Otorhinolaryngology. Stomatology ; Reproducibility of Results ; Spectrometry, Fluorescence - methods ; Staphylococcus aureus - isolation & purification ; Streptococcus pneumoniae - isolation & purification ; Tympanic Membrane</subject><ispartof>Lasers in surgery and medicine, 1994, Vol.14 (2), p.155-163</ispartof><rights>Copyright © 1994 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4398-e6eb94c033c33f6e9c84304c3f672f72b2c2143e021dfd6b2c8321c0300679503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1096-9101%281994%2914%3A2%3C155%3A%3AAID-LSM1900140208%3E3.0.CO%3B2-L$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1096-9101%281994%2914%3A2%3C155%3A%3AAID-LSM1900140208%3E3.0.CO%3B2-L$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4006158$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8183049$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sorrell, Matthew J.</creatorcontrib><creatorcontrib>Tribble, Jerri</creatorcontrib><creatorcontrib>Reinisch, Lou</creatorcontrib><creatorcontrib>Werkhaven, Jay A.</creatorcontrib><creatorcontrib>Ossoff, Robert H.</creatorcontrib><title>Bacteria identification of otitis media with fluorescence spectroscopy</title><title>Lasers in surgery and medicine</title><addtitle>Lasers Surg. Med</addtitle><description>We have investigated the fluorescence profiles of four common pathogens: S. pneumoniae, S. aureus, M. catarrhalis, and H. influenzae. The steady‐state auto fluorescence spectra of bacteria are measured as a function of the incident light from 200 to 700 nm. The spectra for each bacterium are combined into a fluorescence profile or fluorescence finger print. Each bacterium produces a unique in vitro fluorescence profile when measured in a saline suspension. The profiles are reproducible. Suspensions of a bacterial strain, where the identification is not known, can be correctly matched to a small library of previously measured fluorescence profiles using a linear least‐squares fitting algorithm. In addition, we have measured the fluorescence and absorption spectrum of the tympanic membrane removed from a chinchilla. The optical properties of the tympanic membrane and the least‐squares identification process form precept for a non‐invasive, fluorescence based bacterial diagnosis technique to be used in otitis media. © 1994 Wiley‐Liss, Inc.</description><subject>acute otitis media</subject><subject>Algorithms</subject><subject>Animals</subject><subject>bacteria identification</subject><subject>Biological and medical sciences</subject><subject>Chinchilla</subject><subject>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</subject><subject>fluorescence</subject><subject>Haemophilus influenzae - isolation & purification</subject><subject>Humans</subject><subject>Least-Squares Analysis</subject><subject>Medical sciences</subject><subject>Moraxella (Branhamella) catarrhalis - isolation & purification</subject><subject>Non tumoral diseases</subject><subject>noninvasive diagnostics</subject><subject>Otitis Media - microbiology</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Reproducibility of Results</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Streptococcus pneumoniae - isolation & purification</subject><subject>Tympanic Membrane</subject><issn>0196-8092</issn><issn>1096-9101</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE9vEzEQxS0EKqHwEZD2gBA9bJixvX8coFK7paVoIUIF9ThyHK9w2eyGtaOSb4-jRCv1gjh5PO955vnH2AeEKQLwtwgqTxUCvkGl5AnKGX-PWTabnV1fpPXNF1QAKIFDeSqmMK3m73haP2KT8d1jNgGMdQmKP2XPvL8DAMGhOGJHJZYCpJqwy3Ntgh2cTtzSdsE1zujg-i7pm6QPLjifrOwyyvcu_EyadtMP1hvbGZv4tTVh6L3p19vn7EmjW29fHM5j9uPy4_fqU1rPr66rszo1UqgytbldKGlACCNEk1tlShlzmFgXvCn4ghuOUljguGyWebyWgmP0A-SFykAcs9f7ueuh_72xPtDKxThtqzvbbzwVuSzyTGViDGBiQj_YhtaDW-lhSwi040s7TrTjRDu-hJJiL8uIIl96wJcEAVXzqNdx7stDgM0ighmnHoBG_dVB197othl0Z5wfbTJ-BLMy2mhvu3et3f53tn9FeyjEDel-g_PB_hk36OEX5YUoMrr9ekUX5zdVcYvf6LP4CzVdsQo</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Sorrell, Matthew J.</creator><creator>Tribble, Jerri</creator><creator>Reinisch, Lou</creator><creator>Werkhaven, Jay A.</creator><creator>Ossoff, Robert H.</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>Bacteria identification of otitis media with fluorescence spectroscopy</title><author>Sorrell, Matthew J. ; Tribble, Jerri ; Reinisch, Lou ; Werkhaven, Jay A. ; Ossoff, Robert H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4398-e6eb94c033c33f6e9c84304c3f672f72b2c2143e021dfd6b2c8321c0300679503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>acute otitis media</topic><topic>Algorithms</topic><topic>Animals</topic><topic>bacteria identification</topic><topic>Biological and medical sciences</topic><topic>Chinchilla</topic><topic>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</topic><topic>fluorescence</topic><topic>Haemophilus influenzae - isolation & purification</topic><topic>Humans</topic><topic>Least-Squares Analysis</topic><topic>Medical sciences</topic><topic>Moraxella (Branhamella) catarrhalis - isolation & purification</topic><topic>Non tumoral diseases</topic><topic>noninvasive diagnostics</topic><topic>Otitis Media - microbiology</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Reproducibility of Results</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Streptococcus pneumoniae - isolation & purification</topic><topic>Tympanic Membrane</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sorrell, Matthew J.</creatorcontrib><creatorcontrib>Tribble, Jerri</creatorcontrib><creatorcontrib>Reinisch, Lou</creatorcontrib><creatorcontrib>Werkhaven, Jay A.</creatorcontrib><creatorcontrib>Ossoff, Robert H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Lasers in surgery and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sorrell, Matthew J.</au><au>Tribble, Jerri</au><au>Reinisch, Lou</au><au>Werkhaven, Jay A.</au><au>Ossoff, Robert H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacteria identification of otitis media with fluorescence spectroscopy</atitle><jtitle>Lasers in surgery and medicine</jtitle><addtitle>Lasers Surg. Med</addtitle><date>1994</date><risdate>1994</risdate><volume>14</volume><issue>2</issue><spage>155</spage><epage>163</epage><pages>155-163</pages><issn>0196-8092</issn><eissn>1096-9101</eissn><coden>LSMEDI</coden><abstract>We have investigated the fluorescence profiles of four common pathogens: S. pneumoniae, S. aureus, M. catarrhalis, and H. influenzae. The steady‐state auto fluorescence spectra of bacteria are measured as a function of the incident light from 200 to 700 nm. The spectra for each bacterium are combined into a fluorescence profile or fluorescence finger print. Each bacterium produces a unique in vitro fluorescence profile when measured in a saline suspension. The profiles are reproducible. Suspensions of a bacterial strain, where the identification is not known, can be correctly matched to a small library of previously measured fluorescence profiles using a linear least‐squares fitting algorithm. In addition, we have measured the fluorescence and absorption spectrum of the tympanic membrane removed from a chinchilla. The optical properties of the tympanic membrane and the least‐squares identification process form precept for a non‐invasive, fluorescence based bacterial diagnosis technique to be used in otitis media. © 1994 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>8183049</pmid><doi>10.1002/1096-9101(1994)14:2<155::AID-LSM1900140208>3.0.CO;2-L</doi><tpages>9</tpages></addata></record> |
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| ispartof | Lasers in surgery and medicine, 1994, Vol.14 (2), p.155-163 |
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| language | eng |
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| subjects | acute otitis media Algorithms Animals bacteria identification Biological and medical sciences Chinchilla Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology fluorescence Haemophilus influenzae - isolation & purification Humans Least-Squares Analysis Medical sciences Moraxella (Branhamella) catarrhalis - isolation & purification Non tumoral diseases noninvasive diagnostics Otitis Media - microbiology Otorhinolaryngology. Stomatology Reproducibility of Results Spectrometry, Fluorescence - methods Staphylococcus aureus - isolation & purification Streptococcus pneumoniae - isolation & purification Tympanic Membrane |
| title | Bacteria identification of otitis media with fluorescence spectroscopy |
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