β-subunit of human chorionic gonadotropin hormone and firefly luciferase simultaneously synthesized in insect cells using a recombinant baculovirus are differentially expressed and transported

A recombinant baculovirus vAc beta hCG-luc was constructed that carried the cDNAs encoding firefly luciferase (luc) and beta-subunit of human chorionic gonadotropin (beta hCG) placed under the transcriptional control of individual copies of the baculovirus polyhedrin gene promoter. The simple, rapid...

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Veröffentlicht in:	DNA and cell biology 1994-03, Vol.13 (3), p.275-282
Hauptverfasser:	HASNAIN, S. E, NAKHAI, B, EHTESHAM, N. Z, SRIDHAR, P, RANJAN, A, TALWAR, G. P, JHA, P. K
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Baculoviridae - genetics baculovirus Biological and medical sciences Biotechnology Cell Line Chorionic Gonadotropin - analysis Chorionic Gonadotropin - biosynthesis Chorionic Gonadotropin - chemistry Chorionic Gonadotropin, beta Subunit, Human Coleoptera - enzymology Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene Expression Genetic engineering Genetic technics Humans Leydig Cells - drug effects Leydig Cells - physiology Luciferases - biosynthesis Luciferases - metabolism Male Methods. Procedures. Technologies Mice Miscellaneous Moths Peptide Fragments - analysis Peptide Fragments - biosynthesis Peptide Fragments - chemistry Protein Processing, Post-Translational Protein Structure, Secondary Receptors, LH - metabolism Recombinant Proteins - biosynthesis Recombinant Proteins - metabolism Recombinant Proteins - pharmacology Recombination, Genetic Restriction Mapping RNA, Messenger - biosynthesis RNA, Messenger - metabolism Synthetic digonucleotides and genes. Sequencing Thermodynamics Transcription, Genetic Transfection Xenorhabdus luminescens
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