The pectin lyase-encoding gene ( pnl) family from Glomerella cingulata: characterization of pnlA and its expression in yeast

Oligodeoxyribonucleotide primers were designed from conserved amino acid (aa) sequences between pectin lyase D (PNLD) from Aspergillus niger and pectate lyases A and E (PELA/E) from Erwinia chrysanthemi. The polymerase chain reaction (PCR) was used with these primers to amplify genomic DNA from the...

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Veröffentlicht in:	Gene 1994-05, Vol.142 (1), p.141-146
Hauptverfasser:	Templeton, Matthew D., Sharrock, Keith R., Bowen, Joanna K., Crowhurst, ross N., Rikkerink, Erik H.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Ascomycota - enzymology Ascomycota - genetics Base Sequence Biological and medical sciences cDNA cloning Cloning, Molecular Colletotrichum gloeosporioides complementary DNA DNA, Fungal exons Fundamental and applied biological sciences. Psychology fungi genbank/l22857 Gene Expression Genes, Fungal Genes. Genome genetic transformation Glomerella cingulata introns lyases Molecular and cellular biology Molecular genetics Molecular Sequence Data Multigene Family nucleotide sequences PCR pnla gene Polysaccharide-Lyases - genetics Saccharomyces cerevisiae Sequence Homology, Amino Acid structural genes trans-eliminase yeast expression
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