Characteristics of hexokinase, pyruvate kinase, and glucose‐6‐phosphate dehydrogenase during adult and neonatal reticulocyte maturation
Erythrocytes from adults and newborn infants (at term and premature) were separated by Percoll density gradient centrifugation into four fractions of increasing density. Glycolytic enzymes, especially the age‐dependent ones, hexokinase (EC 2.7.1.1, HK), pyruvate kinase (EC 2.7.1.40, PK), and glucose...
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| Veröffentlicht in: | American journal of hematology 1985-11, Vol.20 (3), p.203-215 |
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| creator | Jansen, G. Koenderman, L. Rijksen, G. Cats, B. P. Staal, G. E. J. |
| description | Erythrocytes from adults and newborn infants (at term and premature) were separated by Percoll density gradient centrifugation into four fractions of increasing density. Glycolytic enzymes, especially the age‐dependent ones, hexokinase (EC 2.7.1.1, HK), pyruvate kinase (EC 2.7.1.40, PK), and glucose‐6‐phosphate dehydrogenase (EC 1.1.1.49, G6PD) were studied during reticulocyte maturation and further red cell senescence.
Analysis of the fraction with lowest density showed an almost linear and steep decline of HK, PK, and G6PD activity with a decreasing number of reticulocytes. In the next three fractions of increasing density, the activity decline was far less. These data are therefore illustrative for a biphasic activity decay pattern of HK, PK, and G6PD during both adult and neonatal red cell aging.
The strong decline in HK activity could not be ascribed to the disappearance of a particulate (mitochondrial) bound fraction of the enzyme during reticulocyte maturation. All hexokinase activity in human reticulocytes was found to be cytosolic in contrast with rabbit reticulocytes in which 70% of HK activity was particulate. |
| doi_str_mv | 10.1002/ajh.2830200302 |
| format | Article |
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Analysis of the fraction with lowest density showed an almost linear and steep decline of HK, PK, and G6PD activity with a decreasing number of reticulocytes. In the next three fractions of increasing density, the activity decline was far less. These data are therefore illustrative for a biphasic activity decay pattern of HK, PK, and G6PD during both adult and neonatal red cell aging.
The strong decline in HK activity could not be ascribed to the disappearance of a particulate (mitochondrial) bound fraction of the enzyme during reticulocyte maturation. All hexokinase activity in human reticulocytes was found to be cytosolic in contrast with rabbit reticulocytes in which 70% of HK activity was particulate.</description><identifier>ISSN: 0361-8609</identifier><identifier>EISSN: 1096-8652</identifier><identifier>DOI: 10.1002/ajh.2830200302</identifier><identifier>PMID: 4061449</identifier><identifier>CODEN: AJHEDD</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adult ; Animals ; Biological and medical sciences ; Cell Compartmentation ; Cell Separation ; Electrophoresis, Cellulose Acetate ; Erythrocyte Aging ; Erythrocytes - enzymology ; Fundamental and applied biological sciences. Psychology ; Glucosephosphate Dehydrogenase - blood ; glucose‐6‐phosphate dehydrogenase ; hexokinase ; Hexokinase - blood ; Humans ; Infant, Newborn ; Isoenzymes - blood ; pyruvate kinase ; Pyruvate Kinase - blood ; Rabbits ; Reticulocytes - enzymology ; Reticulocytes - physiology ; Vertebrates: blood, hematopoietic organs, reticuloendothelial system</subject><ispartof>American journal of hematology, 1985-11, Vol.20 (3), p.203-215</ispartof><rights>Copyright © 1985 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4642-250030e609a6f21dcd8896df8544b3bd55eef3238362718ede6f7ca1800c06eb3</citedby><cites>FETCH-LOGICAL-c4642-250030e609a6f21dcd8896df8544b3bd55eef3238362718ede6f7ca1800c06eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fajh.2830200302$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fajh.2830200302$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8662853$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4061449$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jansen, G.</creatorcontrib><creatorcontrib>Koenderman, L.</creatorcontrib><creatorcontrib>Rijksen, G.</creatorcontrib><creatorcontrib>Cats, B. P.</creatorcontrib><creatorcontrib>Staal, G. E. J.</creatorcontrib><title>Characteristics of hexokinase, pyruvate kinase, and glucose‐6‐phosphate dehydrogenase during adult and neonatal reticulocyte maturation</title><title>American journal of hematology</title><addtitle>Am J Hematol</addtitle><description>Erythrocytes from adults and newborn infants (at term and premature) were separated by Percoll density gradient centrifugation into four fractions of increasing density. Glycolytic enzymes, especially the age‐dependent ones, hexokinase (EC 2.7.1.1, HK), pyruvate kinase (EC 2.7.1.40, PK), and glucose‐6‐phosphate dehydrogenase (EC 1.1.1.49, G6PD) were studied during reticulocyte maturation and further red cell senescence.
Analysis of the fraction with lowest density showed an almost linear and steep decline of HK, PK, and G6PD activity with a decreasing number of reticulocytes. In the next three fractions of increasing density, the activity decline was far less. These data are therefore illustrative for a biphasic activity decay pattern of HK, PK, and G6PD during both adult and neonatal red cell aging.
The strong decline in HK activity could not be ascribed to the disappearance of a particulate (mitochondrial) bound fraction of the enzyme during reticulocyte maturation. All hexokinase activity in human reticulocytes was found to be cytosolic in contrast with rabbit reticulocytes in which 70% of HK activity was particulate.</description><subject>Adult</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Compartmentation</subject><subject>Cell Separation</subject><subject>Electrophoresis, Cellulose Acetate</subject><subject>Erythrocyte Aging</subject><subject>Erythrocytes - enzymology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucosephosphate Dehydrogenase - blood</subject><subject>glucose‐6‐phosphate dehydrogenase</subject><subject>hexokinase</subject><subject>Hexokinase - blood</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Isoenzymes - blood</subject><subject>pyruvate kinase</subject><subject>Pyruvate Kinase - blood</subject><subject>Rabbits</subject><subject>Reticulocytes - enzymology</subject><subject>Reticulocytes - physiology</subject><subject>Vertebrates: blood, hematopoietic organs, reticuloendothelial system</subject><issn>0361-8609</issn><issn>1096-8652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD2P1DAQhi0EOpaDlg4pBaJil7GTeJ3ytOI40Ek0UEez9mTjwxsHOwbS0V_Db-SX4GWXg47C9sjzvPPxMvaUw4oDiFd406-EKkEA5OseW3Bo5FLJWtxnCyglzzE0D9mjGG8AOK8UnLGzCiSvqmbBbjc9BtQTBRsnq2Phu6Knb_6THTDSy2KcQ_qCExV_PnAwxc4l7SP9_P5D5jP2Po79gTHUzyb4HR3QwqRgh12BJrnpt2wgP-CErgiUWyXn9ZxFe5xSwMn64TF70KGL9OT0nrOPl68_bK6W1-_fvN1cXC91JSuxFPVhVcpboewEN9oo1UjTqbqqtuXW1DVRV4pSlVKsuSJDsltr5ApAg6Rtec5eHOuOwX9OFKd2b6Mm5zBPmGK7lpWslYIMro6gDj7GQF07BrvHMLcc2oP7bXa__et-Fjw7VU7bPZk7_GR3zj8_5TFqdF3AQdt4hykpharLjDVH7Kt1NP-naXvx7uqfEX4B87SiWw</recordid><startdate>198511</startdate><enddate>198511</enddate><creator>Jansen, G.</creator><creator>Koenderman, L.</creator><creator>Rijksen, G.</creator><creator>Cats, B. P.</creator><creator>Staal, G. E. J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198511</creationdate><title>Characteristics of hexokinase, pyruvate kinase, and glucose‐6‐phosphate dehydrogenase during adult and neonatal reticulocyte maturation</title><author>Jansen, G. ; Koenderman, L. ; Rijksen, G. ; Cats, B. P. ; Staal, G. E. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4642-250030e609a6f21dcd8896df8544b3bd55eef3238362718ede6f7ca1800c06eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Compartmentation</topic><topic>Cell Separation</topic><topic>Electrophoresis, Cellulose Acetate</topic><topic>Erythrocyte Aging</topic><topic>Erythrocytes - enzymology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucosephosphate Dehydrogenase - blood</topic><topic>glucose‐6‐phosphate dehydrogenase</topic><topic>hexokinase</topic><topic>Hexokinase - blood</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Isoenzymes - blood</topic><topic>pyruvate kinase</topic><topic>Pyruvate Kinase - blood</topic><topic>Rabbits</topic><topic>Reticulocytes - enzymology</topic><topic>Reticulocytes - physiology</topic><topic>Vertebrates: blood, hematopoietic organs, reticuloendothelial system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jansen, G.</creatorcontrib><creatorcontrib>Koenderman, L.</creatorcontrib><creatorcontrib>Rijksen, G.</creatorcontrib><creatorcontrib>Cats, B. P.</creatorcontrib><creatorcontrib>Staal, G. E. J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of hematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jansen, G.</au><au>Koenderman, L.</au><au>Rijksen, G.</au><au>Cats, B. P.</au><au>Staal, G. E. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characteristics of hexokinase, pyruvate kinase, and glucose‐6‐phosphate dehydrogenase during adult and neonatal reticulocyte maturation</atitle><jtitle>American journal of hematology</jtitle><addtitle>Am J Hematol</addtitle><date>1985-11</date><risdate>1985</risdate><volume>20</volume><issue>3</issue><spage>203</spage><epage>215</epage><pages>203-215</pages><issn>0361-8609</issn><eissn>1096-8652</eissn><coden>AJHEDD</coden><abstract>Erythrocytes from adults and newborn infants (at term and premature) were separated by Percoll density gradient centrifugation into four fractions of increasing density. Glycolytic enzymes, especially the age‐dependent ones, hexokinase (EC 2.7.1.1, HK), pyruvate kinase (EC 2.7.1.40, PK), and glucose‐6‐phosphate dehydrogenase (EC 1.1.1.49, G6PD) were studied during reticulocyte maturation and further red cell senescence.
Analysis of the fraction with lowest density showed an almost linear and steep decline of HK, PK, and G6PD activity with a decreasing number of reticulocytes. In the next three fractions of increasing density, the activity decline was far less. These data are therefore illustrative for a biphasic activity decay pattern of HK, PK, and G6PD during both adult and neonatal red cell aging.
The strong decline in HK activity could not be ascribed to the disappearance of a particulate (mitochondrial) bound fraction of the enzyme during reticulocyte maturation. All hexokinase activity in human reticulocytes was found to be cytosolic in contrast with rabbit reticulocytes in which 70% of HK activity was particulate.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>4061449</pmid><doi>10.1002/ajh.2830200302</doi><tpages>13</tpages></addata></record> |
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| ispartof | American journal of hematology, 1985-11, Vol.20 (3), p.203-215 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adult Animals Biological and medical sciences Cell Compartmentation Cell Separation Electrophoresis, Cellulose Acetate Erythrocyte Aging Erythrocytes - enzymology Fundamental and applied biological sciences. Psychology Glucosephosphate Dehydrogenase - blood glucose‐6‐phosphate dehydrogenase hexokinase Hexokinase - blood Humans Infant, Newborn Isoenzymes - blood pyruvate kinase Pyruvate Kinase - blood Rabbits Reticulocytes - enzymology Reticulocytes - physiology Vertebrates: blood, hematopoietic organs, reticuloendothelial system |
| title | Characteristics of hexokinase, pyruvate kinase, and glucose‐6‐phosphate dehydrogenase during adult and neonatal reticulocyte maturation |
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