A transient rise in intracellular free calcium is not a sufficient stimulus for respiratory burst activation in human polymorphonuclear leukocytes
Binding of murine monoclonal antibody PMN7C3 to human neutrophils results in a large rapid, dose dependent transient increase in intracellular free calcium as measured by QUIN-2 fluorescence. Unlike other calcium mobilizing agents PMN7C3 does not induce any increase in respiratory burst activity ove...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1985-10, Vol.132 (2), p.674-680 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 132 |
| creator | Apfeldorf, William J. Melnick, David A. Meshulam, Tova Rasmussen, Howard Malech, Harry L. |
| description | Binding of murine monoclonal antibody PMN7C3 to human neutrophils results in a large rapid, dose dependent transient increase in intracellular free calcium as measured by QUIN-2 fluorescence. Unlike other calcium mobilizing agents PMN7C3 does not induce any increase in respiratory burst activity over basal level. The PMN7C3 effect requires multivalent binding. Chelation of extracellular calcium does not significantly decrease the fluorescence transient generated by exposure to PMN7C3. Lowering of basal levels of intracellular free calcium concentration by maintaining QUIN-2-loaded PMN in calcium free medium eliminates the fluorescence transient. The observations demonstrate that a cell surface receptor mediated intracellular free calcium transient may be generated without any associated respiratory burst activation. |
| doi_str_mv | 10.1016/0006-291X(85)91185-4 |
| format | Article |
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Unlike other calcium mobilizing agents PMN7C3 does not induce any increase in respiratory burst activity over basal level. The PMN7C3 effect requires multivalent binding. Chelation of extracellular calcium does not significantly decrease the fluorescence transient generated by exposure to PMN7C3. Lowering of basal levels of intracellular free calcium concentration by maintaining QUIN-2-loaded PMN in calcium free medium eliminates the fluorescence transient. The observations demonstrate that a cell surface receptor mediated intracellular free calcium transient may be generated without any associated respiratory burst activation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(85)91185-4</identifier><identifier>PMID: 3933507</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Antibodies, Monoclonal ; Calcium - metabolism ; Calcium - pharmacology ; Calcium - physiology ; Concanavalin A - pharmacology ; Dose-Response Relationship, Immunologic ; Egtazic Acid - pharmacology ; Humans ; Intracellular Fluid - immunology ; Neutrophils - drug effects ; Neutrophils - metabolism ; Oxygen Consumption ; Zymosan - pharmacology</subject><ispartof>Biochemical and biophysical research communications, 1985-10, Vol.132 (2), p.674-680</ispartof><rights>1985</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-87c009e84fb301b4a3c04e607512451c57e5b2c49d83b53384717319078487493</citedby><cites>FETCH-LOGICAL-c388t-87c009e84fb301b4a3c04e607512451c57e5b2c49d83b53384717319078487493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X85911854$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3933507$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Apfeldorf, William J.</creatorcontrib><creatorcontrib>Melnick, David A.</creatorcontrib><creatorcontrib>Meshulam, Tova</creatorcontrib><creatorcontrib>Rasmussen, Howard</creatorcontrib><creatorcontrib>Malech, Harry L.</creatorcontrib><title>A transient rise in intracellular free calcium is not a sufficient stimulus for respiratory burst activation in human polymorphonuclear leukocytes</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Binding of murine monoclonal antibody PMN7C3 to human neutrophils results in a large rapid, dose dependent transient increase in intracellular free calcium as measured by QUIN-2 fluorescence. 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The observations demonstrate that a cell surface receptor mediated intracellular free calcium transient may be generated without any associated respiratory burst activation.</description><subject>Antibodies, Monoclonal</subject><subject>Calcium - metabolism</subject><subject>Calcium - pharmacology</subject><subject>Calcium - physiology</subject><subject>Concanavalin A - pharmacology</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>Egtazic Acid - pharmacology</subject><subject>Humans</subject><subject>Intracellular Fluid - immunology</subject><subject>Neutrophils - drug effects</subject><subject>Neutrophils - metabolism</subject><subject>Oxygen Consumption</subject><subject>Zymosan - pharmacology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9qFTEUxoMo9bb6BgpZiS5Gk0kySTZCKVqFghsFdyGTe4ZGM5Mxfwr3NfrEZnovXSoEAjnf952T80PoFSXvKaHDB0LI0PWa_nyrxDtNqRIdf4J2lGjS9ZTwp2j3KHmOznP-RQilfNBn6IxpxgSRO3R_iUuyS_awFJx8BuyXdtqbgxBqsAlPCQA7G5yvM_YZL7Fgi3OdJu8ebLn4uYaa8RQTTpBXn2yJ6YDHmnLTuuLvbPFxC8a3dbYLXmM4zDGtt3GpLkDrEqD-ju5QIL9AzyYbMrw83Rfox-dP36--dDffrr9eXd50jilVOiUdIRoUn0ZG6Mgtc4TDQKSgPRfUCQli7B3Xe8VGwZjikkpGNZGKK8k1u0Bvjrlrin8q5GJmn7df2wVizUYOfGCiZ_8VUs51G2VoQn4UuhRzTjCZNfnZpoOhxGzMzAbEbECMEuaBmeHN9vqUX8cZ9o-mE6RW_3isQ9vGnYdk8rZ4B3ufwBWzj_7fDf4CCwSobg</recordid><startdate>19851030</startdate><enddate>19851030</enddate><creator>Apfeldorf, William J.</creator><creator>Melnick, David A.</creator><creator>Meshulam, Tova</creator><creator>Rasmussen, Howard</creator><creator>Malech, Harry L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19851030</creationdate><title>A transient rise in intracellular free calcium is not a sufficient stimulus for respiratory burst activation in human polymorphonuclear leukocytes</title><author>Apfeldorf, William J. ; Melnick, David A. ; Meshulam, Tova ; Rasmussen, Howard ; Malech, Harry L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-87c009e84fb301b4a3c04e607512451c57e5b2c49d83b53384717319078487493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Antibodies, Monoclonal</topic><topic>Calcium - metabolism</topic><topic>Calcium - pharmacology</topic><topic>Calcium - physiology</topic><topic>Concanavalin A - pharmacology</topic><topic>Dose-Response Relationship, Immunologic</topic><topic>Egtazic Acid - pharmacology</topic><topic>Humans</topic><topic>Intracellular Fluid - immunology</topic><topic>Neutrophils - drug effects</topic><topic>Neutrophils - metabolism</topic><topic>Oxygen Consumption</topic><topic>Zymosan - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Apfeldorf, William J.</creatorcontrib><creatorcontrib>Melnick, David A.</creatorcontrib><creatorcontrib>Meshulam, Tova</creatorcontrib><creatorcontrib>Rasmussen, Howard</creatorcontrib><creatorcontrib>Malech, Harry L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Apfeldorf, William J.</au><au>Melnick, David A.</au><au>Meshulam, Tova</au><au>Rasmussen, Howard</au><au>Malech, Harry L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A transient rise in intracellular free calcium is not a sufficient stimulus for respiratory burst activation in human polymorphonuclear leukocytes</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1985-10-30</date><risdate>1985</risdate><volume>132</volume><issue>2</issue><spage>674</spage><epage>680</epage><pages>674-680</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Binding of murine monoclonal antibody PMN7C3 to human neutrophils results in a large rapid, dose dependent transient increase in intracellular free calcium as measured by QUIN-2 fluorescence. Unlike other calcium mobilizing agents PMN7C3 does not induce any increase in respiratory burst activity over basal level. The PMN7C3 effect requires multivalent binding. Chelation of extracellular calcium does not significantly decrease the fluorescence transient generated by exposure to PMN7C3. Lowering of basal levels of intracellular free calcium concentration by maintaining QUIN-2-loaded PMN in calcium free medium eliminates the fluorescence transient. The observations demonstrate that a cell surface receptor mediated intracellular free calcium transient may be generated without any associated respiratory burst activation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>3933507</pmid><doi>10.1016/0006-291X(85)91185-4</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1985-10, Vol.132 (2), p.674-680 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Antibodies, Monoclonal Calcium - metabolism Calcium - pharmacology Calcium - physiology Concanavalin A - pharmacology Dose-Response Relationship, Immunologic Egtazic Acid - pharmacology Humans Intracellular Fluid - immunology Neutrophils - drug effects Neutrophils - metabolism Oxygen Consumption Zymosan - pharmacology |
| title | A transient rise in intracellular free calcium is not a sufficient stimulus for respiratory burst activation in human polymorphonuclear leukocytes |
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