Abnormal expression of plasminogen activators in aortic aneurysmal and occlusive disease
Purpose and Methods : Aortic aneurysms are characterized by the destruction of the extracellular matrix of the media, whereas occlusive disease involves excess matrix accumulation within the intima. Plasmin degrades extracellular matrix directly and indirectly by activation of latent metalloenzymes....
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| Veröffentlicht in: | Journal of vascular surgery 1994-05, Vol.19 (5), p.865-872 |
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| creator | Reilly, Jeffrey M. Sicard, Gregorio A. Lucore, Charles L. |
| description | Purpose and Methods : Aortic aneurysms are characterized by the destruction of the extracellular matrix of the media, whereas occlusive disease involves excess matrix accumulation within the intima. Plasmin degrades extracellular matrix directly and indirectly by activation of latent metalloenzymes. To determine the expression of tissue- and urokinase-type plasminogen activators, immunoassay, fibrin autography, Northern analysis, and immunohistochemistry were performed on specimens of aneurysmal (
n = 12), occlusive (
n = 8), and healthy (
n = 6) aorta.
Results: Immunoassay of tissue-type plasminogen activator revealed 8.7 ± 0.9 ng tissue-type plasminogen activator/mg extracted protein in aneurysmal aorta, 5.7 ± 0.3 ng/mg in normal aorta, and 2.5 ± 0.3 ng/mg in occlusive aorta (
p < 0.05 for comparisons between all groups). No urokinase-type plasminogen activator antigen was detected by urokinase-type plasminogen activator immunoassay. Fibrin autography exhibited lytic activity at 64 kDa and 54 kDa attributable to tissue-type plasminogen activator and urokinase-type plasminogen activator. The vast majority of fibrinolysis was secondary to free tissue-type plasminogen activator and was greatest in aneurysmal disease and least in occlusive disease. There was only a small amount of lysis secondary to urokinase-type plasminogen activator. Expression of tissue-type plasminogen activator and urokinase-type plasminogen activators mRNA was comparable in aneurysmal and occlusive aortas. In contrast to occlusive disease, aneurysms had an inflammatory cell infiltrate characterized by the expression of urokinase-type plasminogen activator by specific mononuclear cells. Tissue-type plasminogen activator expression was evident in the intima of normal and diseased aorta and in the media of diseased aorta.
Conclusion : Differential expression of plasminogen activators within the arterial wall may contribute to the unique pathogenesis of aneurysmal and occlusive aortic disease. (J V
ASC S
URG 1994;19:865-72) |
| doi_str_mv | 10.1016/S0741-5214(94)70012-5 |
| format | Article |
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n = 12), occlusive (
n = 8), and healthy (
n = 6) aorta.
Results: Immunoassay of tissue-type plasminogen activator revealed 8.7 ± 0.9 ng tissue-type plasminogen activator/mg extracted protein in aneurysmal aorta, 5.7 ± 0.3 ng/mg in normal aorta, and 2.5 ± 0.3 ng/mg in occlusive aorta (
p < 0.05 for comparisons between all groups). No urokinase-type plasminogen activator antigen was detected by urokinase-type plasminogen activator immunoassay. Fibrin autography exhibited lytic activity at 64 kDa and 54 kDa attributable to tissue-type plasminogen activator and urokinase-type plasminogen activator. The vast majority of fibrinolysis was secondary to free tissue-type plasminogen activator and was greatest in aneurysmal disease and least in occlusive disease. There was only a small amount of lysis secondary to urokinase-type plasminogen activator. Expression of tissue-type plasminogen activator and urokinase-type plasminogen activators mRNA was comparable in aneurysmal and occlusive aortas. In contrast to occlusive disease, aneurysms had an inflammatory cell infiltrate characterized by the expression of urokinase-type plasminogen activator by specific mononuclear cells. Tissue-type plasminogen activator expression was evident in the intima of normal and diseased aorta and in the media of diseased aorta.
Conclusion : Differential expression of plasminogen activators within the arterial wall may contribute to the unique pathogenesis of aneurysmal and occlusive aortic disease. (J V
ASC S
URG 1994;19:865-72)</description><identifier>ISSN: 0741-5214</identifier><identifier>EISSN: 1097-6809</identifier><identifier>DOI: 10.1016/S0741-5214(94)70012-5</identifier><identifier>PMID: 8170041</identifier><identifier>CODEN: JVSUES</identifier><language>eng</language><publisher>New York, NY: Mosby, Inc</publisher><subject>Adult ; Aged ; Aorta, Abdominal - chemistry ; Aorta, Abdominal - enzymology ; Aortic Aneurysm, Abdominal - enzymology ; Aortic Aneurysm, Abdominal - etiology ; Aortic Diseases - enzymology ; Aortic Diseases - etiology ; Arterial Occlusive Diseases - enzymology ; Arterial Occlusive Diseases - etiology ; Autoradiography - methods ; Autoradiography - statistics & numerical data ; Biological and medical sciences ; Blood and lymphatic vessels ; Blotting, Northern - methods ; Blotting, Northern - statistics & numerical data ; Cardiology. Vascular system ; Diseases of the aorta ; DNA Probes ; Electrophoresis, Polyacrylamide Gel - methods ; Electrophoresis, Polyacrylamide Gel - statistics & numerical data ; Fibrin - analysis ; Humans ; Immunohistochemistry - methods ; Immunohistochemistry - statistics & numerical data ; Medical sciences ; Middle Aged ; Plasminogen Activators - analysis ; Plasminogen Activators - isolation & purification ; Plasminogen Activators - metabolism</subject><ispartof>Journal of vascular surgery, 1994-05, Vol.19 (5), p.865-872</ispartof><rights>1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-d8c9afee1c0552a288c5e991762e50d1b890338e9c6a614b5ab07b91fd2331bf3</citedby><cites>FETCH-LOGICAL-c502t-d8c9afee1c0552a288c5e991762e50d1b890338e9c6a614b5ab07b91fd2331bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0741521494700125$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4056836$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8170041$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reilly, Jeffrey M.</creatorcontrib><creatorcontrib>Sicard, Gregorio A.</creatorcontrib><creatorcontrib>Lucore, Charles L.</creatorcontrib><creatorcontrib>From the Department of Surgery, Washington University School of Medicine, St. Louis, and the Department of Medicine, Southern Illinois University School of Medicine, Springfield</creatorcontrib><title>Abnormal expression of plasminogen activators in aortic aneurysmal and occlusive disease</title><title>Journal of vascular surgery</title><addtitle>J Vasc Surg</addtitle><description>Purpose and Methods : Aortic aneurysms are characterized by the destruction of the extracellular matrix of the media, whereas occlusive disease involves excess matrix accumulation within the intima. Plasmin degrades extracellular matrix directly and indirectly by activation of latent metalloenzymes. To determine the expression of tissue- and urokinase-type plasminogen activators, immunoassay, fibrin autography, Northern analysis, and immunohistochemistry were performed on specimens of aneurysmal (
n = 12), occlusive (
n = 8), and healthy (
n = 6) aorta.
Results: Immunoassay of tissue-type plasminogen activator revealed 8.7 ± 0.9 ng tissue-type plasminogen activator/mg extracted protein in aneurysmal aorta, 5.7 ± 0.3 ng/mg in normal aorta, and 2.5 ± 0.3 ng/mg in occlusive aorta (
p < 0.05 for comparisons between all groups). No urokinase-type plasminogen activator antigen was detected by urokinase-type plasminogen activator immunoassay. Fibrin autography exhibited lytic activity at 64 kDa and 54 kDa attributable to tissue-type plasminogen activator and urokinase-type plasminogen activator. The vast majority of fibrinolysis was secondary to free tissue-type plasminogen activator and was greatest in aneurysmal disease and least in occlusive disease. There was only a small amount of lysis secondary to urokinase-type plasminogen activator. Expression of tissue-type plasminogen activator and urokinase-type plasminogen activators mRNA was comparable in aneurysmal and occlusive aortas. In contrast to occlusive disease, aneurysms had an inflammatory cell infiltrate characterized by the expression of urokinase-type plasminogen activator by specific mononuclear cells. Tissue-type plasminogen activator expression was evident in the intima of normal and diseased aorta and in the media of diseased aorta.
Conclusion : Differential expression of plasminogen activators within the arterial wall may contribute to the unique pathogenesis of aneurysmal and occlusive aortic disease. (J V
ASC S
URG 1994;19:865-72)</description><subject>Adult</subject><subject>Aged</subject><subject>Aorta, Abdominal - chemistry</subject><subject>Aorta, Abdominal - enzymology</subject><subject>Aortic Aneurysm, Abdominal - enzymology</subject><subject>Aortic Aneurysm, Abdominal - etiology</subject><subject>Aortic Diseases - enzymology</subject><subject>Aortic Diseases - etiology</subject><subject>Arterial Occlusive Diseases - enzymology</subject><subject>Arterial Occlusive Diseases - etiology</subject><subject>Autoradiography - methods</subject><subject>Autoradiography - statistics & numerical data</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Blotting, Northern - methods</subject><subject>Blotting, Northern - statistics & numerical data</subject><subject>Cardiology. Vascular system</subject><subject>Diseases of the aorta</subject><subject>DNA Probes</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Electrophoresis, Polyacrylamide Gel - statistics & numerical data</subject><subject>Fibrin - analysis</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Immunohistochemistry - statistics & numerical data</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Plasminogen Activators - analysis</subject><subject>Plasminogen Activators - isolation & purification</subject><subject>Plasminogen Activators - metabolism</subject><issn>0741-5214</issn><issn>1097-6809</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVpSTbb_oSAD6E0Bzca25KlUwkhaQKBHJJAb0KWx0HBtrYae-n--2g_2GtPwzDPOzM8jJ0D_wkc5NUzryvIRQHVD11d1pxDkYtPbAFc17lUXH9miyNyys6I3hMDQtUn7ERBClSwYH-umzHEwfYZ_ltFJPJhzEKXrXpLgx_DG46ZdZNf2ylEynzqQpy8y-yIc9zQNmnHNgvO9TP5NWatJ7SEX9mXzvaE3w51yV7vbl9u7vPHp98PN9ePuRO8mPJWOW07RHBciMIWSjmBWkMtCxS8hUZpXpYKtZNWQtUI2_C60dC1RVlC05VL9n2_dxXD3xlpMoMnh32fHgwzmVpWshBpx5KJPehiIIrYmVX0g40bA9xsjZqdUbPVZXRldkaNSLnzw4G5GbA9pg4K0_ziMLfkbN9FOzpPR6ziQqpSJuzXHsMkY-0xGnIeR4etj-gm0wb_n0c-AIgwk0o</recordid><startdate>19940501</startdate><enddate>19940501</enddate><creator>Reilly, Jeffrey M.</creator><creator>Sicard, Gregorio A.</creator><creator>Lucore, Charles L.</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940501</creationdate><title>Abnormal expression of plasminogen activators in aortic aneurysmal and occlusive disease</title><author>Reilly, Jeffrey M. ; Sicard, Gregorio A. ; Lucore, Charles L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-d8c9afee1c0552a288c5e991762e50d1b890338e9c6a614b5ab07b91fd2331bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aorta, Abdominal - chemistry</topic><topic>Aorta, Abdominal - enzymology</topic><topic>Aortic Aneurysm, Abdominal - enzymology</topic><topic>Aortic Aneurysm, Abdominal - etiology</topic><topic>Aortic Diseases - enzymology</topic><topic>Aortic Diseases - etiology</topic><topic>Arterial Occlusive Diseases - enzymology</topic><topic>Arterial Occlusive Diseases - etiology</topic><topic>Autoradiography - methods</topic><topic>Autoradiography - statistics & numerical data</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Blotting, Northern - methods</topic><topic>Blotting, Northern - statistics & numerical data</topic><topic>Cardiology. Vascular system</topic><topic>Diseases of the aorta</topic><topic>DNA Probes</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Electrophoresis, Polyacrylamide Gel - statistics & numerical data</topic><topic>Fibrin - analysis</topic><topic>Humans</topic><topic>Immunohistochemistry - methods</topic><topic>Immunohistochemistry - statistics & numerical data</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Plasminogen Activators - analysis</topic><topic>Plasminogen Activators - isolation & purification</topic><topic>Plasminogen Activators - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reilly, Jeffrey M.</creatorcontrib><creatorcontrib>Sicard, Gregorio A.</creatorcontrib><creatorcontrib>Lucore, Charles L.</creatorcontrib><creatorcontrib>From the Department of Surgery, Washington University School of Medicine, St. Louis, and the Department of Medicine, Southern Illinois University School of Medicine, Springfield</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of vascular surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reilly, Jeffrey M.</au><au>Sicard, Gregorio A.</au><au>Lucore, Charles L.</au><aucorp>From the Department of Surgery, Washington University School of Medicine, St. Louis, and the Department of Medicine, Southern Illinois University School of Medicine, Springfield</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abnormal expression of plasminogen activators in aortic aneurysmal and occlusive disease</atitle><jtitle>Journal of vascular surgery</jtitle><addtitle>J Vasc Surg</addtitle><date>1994-05-01</date><risdate>1994</risdate><volume>19</volume><issue>5</issue><spage>865</spage><epage>872</epage><pages>865-872</pages><issn>0741-5214</issn><eissn>1097-6809</eissn><coden>JVSUES</coden><abstract>Purpose and Methods : Aortic aneurysms are characterized by the destruction of the extracellular matrix of the media, whereas occlusive disease involves excess matrix accumulation within the intima. Plasmin degrades extracellular matrix directly and indirectly by activation of latent metalloenzymes. To determine the expression of tissue- and urokinase-type plasminogen activators, immunoassay, fibrin autography, Northern analysis, and immunohistochemistry were performed on specimens of aneurysmal (
n = 12), occlusive (
n = 8), and healthy (
n = 6) aorta.
Results: Immunoassay of tissue-type plasminogen activator revealed 8.7 ± 0.9 ng tissue-type plasminogen activator/mg extracted protein in aneurysmal aorta, 5.7 ± 0.3 ng/mg in normal aorta, and 2.5 ± 0.3 ng/mg in occlusive aorta (
p < 0.05 for comparisons between all groups). No urokinase-type plasminogen activator antigen was detected by urokinase-type plasminogen activator immunoassay. Fibrin autography exhibited lytic activity at 64 kDa and 54 kDa attributable to tissue-type plasminogen activator and urokinase-type plasminogen activator. The vast majority of fibrinolysis was secondary to free tissue-type plasminogen activator and was greatest in aneurysmal disease and least in occlusive disease. There was only a small amount of lysis secondary to urokinase-type plasminogen activator. Expression of tissue-type plasminogen activator and urokinase-type plasminogen activators mRNA was comparable in aneurysmal and occlusive aortas. In contrast to occlusive disease, aneurysms had an inflammatory cell infiltrate characterized by the expression of urokinase-type plasminogen activator by specific mononuclear cells. Tissue-type plasminogen activator expression was evident in the intima of normal and diseased aorta and in the media of diseased aorta.
Conclusion : Differential expression of plasminogen activators within the arterial wall may contribute to the unique pathogenesis of aneurysmal and occlusive aortic disease. (J V
ASC S
URG 1994;19:865-72)</abstract><cop>New York, NY</cop><pub>Mosby, Inc</pub><pmid>8170041</pmid><doi>10.1016/S0741-5214(94)70012-5</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adult Aged Aorta, Abdominal - chemistry Aorta, Abdominal - enzymology Aortic Aneurysm, Abdominal - enzymology Aortic Aneurysm, Abdominal - etiology Aortic Diseases - enzymology Aortic Diseases - etiology Arterial Occlusive Diseases - enzymology Arterial Occlusive Diseases - etiology Autoradiography - methods Autoradiography - statistics & numerical data Biological and medical sciences Blood and lymphatic vessels Blotting, Northern - methods Blotting, Northern - statistics & numerical data Cardiology. Vascular system Diseases of the aorta DNA Probes Electrophoresis, Polyacrylamide Gel - methods Electrophoresis, Polyacrylamide Gel - statistics & numerical data Fibrin - analysis Humans Immunohistochemistry - methods Immunohistochemistry - statistics & numerical data Medical sciences Middle Aged Plasminogen Activators - analysis Plasminogen Activators - isolation & purification Plasminogen Activators - metabolism |
| title | Abnormal expression of plasminogen activators in aortic aneurysmal and occlusive disease |
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