CaBP9K levels during the luteal and follicular phases of the estrous cycle in the bovine uterus
The expression of calbindin-D(9K) (CaBP(9K)) and calbindin-D(28K) (CaBP(28K)) genes in the reproductive system is well established for rodent and avian species, but not for domestic livestock. This investigation expanded the study of these proteins to include the bovine uterus and examined the level...
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| Veröffentlicht in: | Biology of reproduction 1994-03, Vol.50 (3), p.561-571 |
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| creator | Inpanbutr, N Miller, E.K Petroff, B.K Iacopino, A.M |
| description | The expression of calbindin-D(9K) (CaBP(9K)) and calbindin-D(28K) (CaBP(28K)) genes in the reproductive system is well established for rodent and avian species, but not for domestic livestock. This investigation expanded the study of these proteins to include the bovine uterus and examined the levels of CaBP(9K) and CaBP(9K) mRNA in the nonpregnant bovine uterus during the estrous cycle. Immunohistochemical studies revealed that CaBP(9K) was present in all uterine glandular and luminal epithelial cells. In contrast, the closely related calcium binding protein CaBP(28K) was present in only one to two glandular cells in all the samples examined. Neither protein was localized in the myometrium or in the stromal cells of the endometrium. RIA and dot blot hybridization were used to quantify the amount of CaBP(9K) and CaBP(9K) mRNA. The levels of both the protein and its mRNA were threefold higher during the luteal phase than during the follicular phase. RIA was also used to determine bovine uterine levels of 17 beta-estradiol and progesterone. Progesterone levels were higher during the luteal phase than during the follicular phase, while 17 beta-estradiol levels were higher during the follicular phase. This investigation represents the first characterization of CaBP(9K) gene expression in the bovine uterus. It demonstrated that the expression of CaBP(9K) and CaBP(9K) mRNA was greatest during the progesterone-dominated luteal phase of the bovine estrous cycle. These results indicated that CaBP(9K) may be involved in uterine glandular function during the luteal phase |
| doi_str_mv | 10.1095/biolreprod50.3.561 |
| format | Article |
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This investigation expanded the study of these proteins to include the bovine uterus and examined the levels of CaBP(9K) and CaBP(9K) mRNA in the nonpregnant bovine uterus during the estrous cycle. Immunohistochemical studies revealed that CaBP(9K) was present in all uterine glandular and luminal epithelial cells. In contrast, the closely related calcium binding protein CaBP(28K) was present in only one to two glandular cells in all the samples examined. Neither protein was localized in the myometrium or in the stromal cells of the endometrium. RIA and dot blot hybridization were used to quantify the amount of CaBP(9K) and CaBP(9K) mRNA. The levels of both the protein and its mRNA were threefold higher during the luteal phase than during the follicular phase. RIA was also used to determine bovine uterine levels of 17 beta-estradiol and progesterone. Progesterone levels were higher during the luteal phase than during the follicular phase, while 17 beta-estradiol levels were higher during the follicular phase. This investigation represents the first characterization of CaBP(9K) gene expression in the bovine uterus. It demonstrated that the expression of CaBP(9K) and CaBP(9K) mRNA was greatest during the progesterone-dominated luteal phase of the bovine estrous cycle. These results indicated that CaBP(9K) may be involved in uterine glandular function during the luteal phase</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod50.3.561</identifier><identifier>PMID: 8167228</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; ARN MENSAJERO ; ARN MESSAGER ; Biological and medical sciences ; Calbindins ; Cattle - metabolism ; CICLO ESTRAL ; CYCLE OESTRAL ; Epithelium - metabolism ; Estradiol - metabolism ; ESTROGENOS ; Estrus - physiology ; EXPRESION GENICA ; EXPRESSION DES GENES ; Female ; Follicular Phase - physiology ; Fundamental and applied biological sciences. Psychology ; Hormone metabolism and regulation ; Immunoenzyme Techniques ; Luteal Phase - physiology ; Mammalian female genital system ; Nucleic Acid Hybridization ; OESTROGENE ; PROGESTERONA ; PROGESTERONE ; Progesterone - metabolism ; PROTEINAS ; PROTEINE ; Radioimmunoassay ; RNA, Messenger - metabolism ; S100 Calcium Binding Protein G - genetics ; S100 Calcium Binding Protein G - metabolism ; Tissue Distribution ; UTERO ; UTERUS ; Uterus - metabolism ; VACA ; VACHE ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1994-03, Vol.50 (3), p.561-571</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4061175$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8167228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inpanbutr, N</creatorcontrib><creatorcontrib>Miller, E.K</creatorcontrib><creatorcontrib>Petroff, B.K</creatorcontrib><creatorcontrib>Iacopino, A.M</creatorcontrib><title>CaBP9K levels during the luteal and follicular phases of the estrous cycle in the bovine uterus</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The expression of calbindin-D(9K) (CaBP(9K)) and calbindin-D(28K) (CaBP(28K)) genes in the reproductive system is well established for rodent and avian species, but not for domestic livestock. This investigation expanded the study of these proteins to include the bovine uterus and examined the levels of CaBP(9K) and CaBP(9K) mRNA in the nonpregnant bovine uterus during the estrous cycle. Immunohistochemical studies revealed that CaBP(9K) was present in all uterine glandular and luminal epithelial cells. In contrast, the closely related calcium binding protein CaBP(28K) was present in only one to two glandular cells in all the samples examined. Neither protein was localized in the myometrium or in the stromal cells of the endometrium. RIA and dot blot hybridization were used to quantify the amount of CaBP(9K) and CaBP(9K) mRNA. The levels of both the protein and its mRNA were threefold higher during the luteal phase than during the follicular phase. RIA was also used to determine bovine uterine levels of 17 beta-estradiol and progesterone. Progesterone levels were higher during the luteal phase than during the follicular phase, while 17 beta-estradiol levels were higher during the follicular phase. This investigation represents the first characterization of CaBP(9K) gene expression in the bovine uterus. It demonstrated that the expression of CaBP(9K) and CaBP(9K) mRNA was greatest during the progesterone-dominated luteal phase of the bovine estrous cycle. These results indicated that CaBP(9K) may be involved in uterine glandular function during the luteal phase</description><subject>Animals</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>Biological and medical sciences</subject><subject>Calbindins</subject><subject>Cattle - metabolism</subject><subject>CICLO ESTRAL</subject><subject>CYCLE OESTRAL</subject><subject>Epithelium - metabolism</subject><subject>Estradiol - metabolism</subject><subject>ESTROGENOS</subject><subject>Estrus - physiology</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Female</subject><subject>Follicular Phase - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormone metabolism and regulation</subject><subject>Immunoenzyme Techniques</subject><subject>Luteal Phase - physiology</subject><subject>Mammalian female genital system</subject><subject>Nucleic Acid Hybridization</subject><subject>OESTROGENE</subject><subject>PROGESTERONA</subject><subject>PROGESTERONE</subject><subject>Progesterone - metabolism</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Radioimmunoassay</subject><subject>RNA, Messenger - metabolism</subject><subject>S100 Calcium Binding Protein G - genetics</subject><subject>S100 Calcium Binding Protein G - metabolism</subject><subject>Tissue Distribution</subject><subject>UTERO</subject><subject>UTERUS</subject><subject>Uterus - metabolism</subject><subject>VACA</subject><subject>VACHE</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kVtrGzEQhUVpSJ20fyBQ0EObt3Wk1W312JrciCGFNs9iVjtrq8i7ruSN8b-viE2eZpjzzWE4Q8gVZ3POrLppwxgTbtPYKTYXc6X5BzLjqraVqXXzkcwYY7oSQotP5CLnv4xxKWpxTs4brk1dNzPiFvDzl32iEV8xZtpNKQwrulsjjdMOIVIYOtqPMQY_RUh0u4aMmY79G4N5l8YpU3_wEWkY3obt-BoGpGU9TfkzOeshZvxyqpfk5e72z-KhWj7fPy5-LKu-5npXScWwdJ2WwvdMKW9b09bCet1KDtZ2tsUavLEa0PdScuY76VVrLVPGQycuyfXRt6Txbyp3uU3IHmOEAcuFzmipORNNAb-ewKndYOe2KWwgHdwpkaJ_O-mQPcQ-weBDfsck05wbVbDvR2wdVut9SOjyBmIspsLt93vFnHDlH4W7OnI9jA5WqVi9_LaKGdMI8R8dXIdd</recordid><startdate>19940301</startdate><enddate>19940301</enddate><creator>Inpanbutr, N</creator><creator>Miller, E.K</creator><creator>Petroff, B.K</creator><creator>Iacopino, A.M</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19940301</creationdate><title>CaBP9K levels during the luteal and follicular phases of the estrous cycle in the bovine uterus</title><author>Inpanbutr, N ; Miller, E.K ; Petroff, B.K ; Iacopino, A.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f216t-450ef21d643cf055c9b7b239c6b41a99d9be2ac796aecf4410cd4c5b99057cad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Biological and medical sciences</topic><topic>Calbindins</topic><topic>Cattle - metabolism</topic><topic>CICLO ESTRAL</topic><topic>CYCLE OESTRAL</topic><topic>Epithelium - metabolism</topic><topic>Estradiol - metabolism</topic><topic>ESTROGENOS</topic><topic>Estrus - physiology</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Female</topic><topic>Follicular Phase - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormone metabolism and regulation</topic><topic>Immunoenzyme Techniques</topic><topic>Luteal Phase - physiology</topic><topic>Mammalian female genital system</topic><topic>Nucleic Acid Hybridization</topic><topic>OESTROGENE</topic><topic>PROGESTERONA</topic><topic>PROGESTERONE</topic><topic>Progesterone - metabolism</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Radioimmunoassay</topic><topic>RNA, Messenger - metabolism</topic><topic>S100 Calcium Binding Protein G - genetics</topic><topic>S100 Calcium Binding Protein G - metabolism</topic><topic>Tissue Distribution</topic><topic>UTERO</topic><topic>UTERUS</topic><topic>Uterus - metabolism</topic><topic>VACA</topic><topic>VACHE</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inpanbutr, N</creatorcontrib><creatorcontrib>Miller, E.K</creatorcontrib><creatorcontrib>Petroff, B.K</creatorcontrib><creatorcontrib>Iacopino, A.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inpanbutr, N</au><au>Miller, E.K</au><au>Petroff, B.K</au><au>Iacopino, A.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CaBP9K levels during the luteal and follicular phases of the estrous cycle in the bovine uterus</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1994-03-01</date><risdate>1994</risdate><volume>50</volume><issue>3</issue><spage>561</spage><epage>571</epage><pages>561-571</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>The expression of calbindin-D(9K) (CaBP(9K)) and calbindin-D(28K) (CaBP(28K)) genes in the reproductive system is well established for rodent and avian species, but not for domestic livestock. This investigation expanded the study of these proteins to include the bovine uterus and examined the levels of CaBP(9K) and CaBP(9K) mRNA in the nonpregnant bovine uterus during the estrous cycle. Immunohistochemical studies revealed that CaBP(9K) was present in all uterine glandular and luminal epithelial cells. In contrast, the closely related calcium binding protein CaBP(28K) was present in only one to two glandular cells in all the samples examined. Neither protein was localized in the myometrium or in the stromal cells of the endometrium. RIA and dot blot hybridization were used to quantify the amount of CaBP(9K) and CaBP(9K) mRNA. The levels of both the protein and its mRNA were threefold higher during the luteal phase than during the follicular phase. RIA was also used to determine bovine uterine levels of 17 beta-estradiol and progesterone. Progesterone levels were higher during the luteal phase than during the follicular phase, while 17 beta-estradiol levels were higher during the follicular phase. This investigation represents the first characterization of CaBP(9K) gene expression in the bovine uterus. It demonstrated that the expression of CaBP(9K) and CaBP(9K) mRNA was greatest during the progesterone-dominated luteal phase of the bovine estrous cycle. These results indicated that CaBP(9K) may be involved in uterine glandular function during the luteal phase</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>8167228</pmid><doi>10.1095/biolreprod50.3.561</doi><tpages>11</tpages></addata></record> |
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| ispartof | Biology of reproduction, 1994-03, Vol.50 (3), p.561-571 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_76461038 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals ARN MENSAJERO ARN MESSAGER Biological and medical sciences Calbindins Cattle - metabolism CICLO ESTRAL CYCLE OESTRAL Epithelium - metabolism Estradiol - metabolism ESTROGENOS Estrus - physiology EXPRESION GENICA EXPRESSION DES GENES Female Follicular Phase - physiology Fundamental and applied biological sciences. Psychology Hormone metabolism and regulation Immunoenzyme Techniques Luteal Phase - physiology Mammalian female genital system Nucleic Acid Hybridization OESTROGENE PROGESTERONA PROGESTERONE Progesterone - metabolism PROTEINAS PROTEINE Radioimmunoassay RNA, Messenger - metabolism S100 Calcium Binding Protein G - genetics S100 Calcium Binding Protein G - metabolism Tissue Distribution UTERO UTERUS Uterus - metabolism VACA VACHE Vertebrates: reproduction |
| title | CaBP9K levels during the luteal and follicular phases of the estrous cycle in the bovine uterus |
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