Molecular cloning of human Syk. A B cell protein-tyrosine kinase associated with the surface immunoglobulin M-B cell receptor complex
B cell antigen receptors are multicomponent complexes consisting of the surface immunoglobulin and accessory molecules with associating protein-tyrosine kinases. A spleen tyrosine kinase, Syk, in porcine B cells and a 72-kDa protein-tyrosine kinase, PTK72, in murine B cells associate with the B cell...
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| Veröffentlicht in: | The Journal of biological chemistry 1994-04, Vol.269 (16), p.12310-12319 |
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| creator | CHE-LEUNG LAW SIDORENKO, S. P CHANDRAN, K. A DRAVES, K. E CHAN, A. C WEISS, A EDELHOFF, S DISTECHE, C. M CLARK, E. A |
| description | B cell antigen receptors are multicomponent complexes consisting of the surface immunoglobulin and accessory molecules with
associating protein-tyrosine kinases. A spleen tyrosine kinase, Syk, in porcine B cells and a 72-kDa protein-tyrosine kinase,
PTK72, in murine B cells associate with the B cell antigen receptor. Herein, we report the isolation of a full-length cDNA
encoding the human homologue of Syk. This cDNA predicted a polypeptide consisting of two NH2-terminal SH2 domains and a COOH-terminal
tyrosine kinase domain. Syk is highly conserved between human and swine and is homologous to the T cell-associated protein-tyrosine
kinase ZAP-70. Both Syk mRNA and protein were detected in cells derived from multiple hematopoietic lineages. Within the B
cell compartment, Syk was expressed from pro-B cells to plasma cells. In vitro kinase assays conducted on the human Syk protein
isolated from B cells revealed the presence of autophosphorylation activity on Syk tyrosine residues. Tyrosine phosphorylation
of Syk associating with the B cell receptor complex in human was augmented rapidly after surface immunoglobulin cross-linking.
The human SYK locus was mapped to chromosome 9 at band q22. |
| doi_str_mv | 10.1016/S0021-9258(17)32717-5 |
| format | Article |
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associating protein-tyrosine kinases. A spleen tyrosine kinase, Syk, in porcine B cells and a 72-kDa protein-tyrosine kinase,
PTK72, in murine B cells associate with the B cell antigen receptor. Herein, we report the isolation of a full-length cDNA
encoding the human homologue of Syk. This cDNA predicted a polypeptide consisting of two NH2-terminal SH2 domains and a COOH-terminal
tyrosine kinase domain. Syk is highly conserved between human and swine and is homologous to the T cell-associated protein-tyrosine
kinase ZAP-70. Both Syk mRNA and protein were detected in cells derived from multiple hematopoietic lineages. Within the B
cell compartment, Syk was expressed from pro-B cells to plasma cells. In vitro kinase assays conducted on the human Syk protein
isolated from B cells revealed the presence of autophosphorylation activity on Syk tyrosine residues. Tyrosine phosphorylation
of Syk associating with the B cell receptor complex in human was augmented rapidly after surface immunoglobulin cross-linking.
The human SYK locus was mapped to chromosome 9 at band q22.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(17)32717-5</identifier><identifier>PMID: 8163536</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Amino Acid Sequence ; Analytical, structural and metabolic biochemistry ; Animals ; association ; B-Lymphocytes - enzymology ; B-Lymphocytes - immunology ; Base Sequence ; Biological and medical sciences ; cDNA ; Cell Line ; cell surface ; Chromosome Mapping ; Chromosomes, Human, Pair 9 ; Cloning, Molecular - methods ; Conserved Sequence ; DNA, Complementary - analysis ; Enzyme Precursors - biosynthesis ; Enzyme Precursors - genetics ; Enzyme Precursors - isolation & purification ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; genes ; Humans ; immunoglobulin M ; Intracellular Signaling Peptides and Proteins ; lymphocytes B ; man ; Mice ; Molecular Sequence Data ; nucleotide sequence ; Phosphorylation ; prediction ; protein-tyrosine kinase ; Protein-Tyrosine Kinases - biosynthesis ; Protein-Tyrosine Kinases - genetics ; Protein-Tyrosine Kinases - isolation & purification ; Receptors, Antigen, B-Cell - isolation & purification ; Receptors, Antigen, B-Cell - metabolism ; Restriction Mapping ; RNA, Messenger - analysis ; RNA, Messenger - biosynthesis ; Sequence Homology, Amino Acid ; Spleen - enzymology ; Swine ; Syk Kinase ; T-Lymphocytes - immunology ; Transfection ; Transferases</subject><ispartof>The Journal of biological chemistry, 1994-04, Vol.269 (16), p.12310-12319</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-47d4b8a644cd39e2abf707ce94854816e247edad717bcdd1aa54a7f4009c46033</citedby><cites>FETCH-LOGICAL-c440t-47d4b8a644cd39e2abf707ce94854816e247edad717bcdd1aa54a7f4009c46033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4153701$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8163536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHE-LEUNG LAW</creatorcontrib><creatorcontrib>SIDORENKO, S. P</creatorcontrib><creatorcontrib>CHANDRAN, K. A</creatorcontrib><creatorcontrib>DRAVES, K. E</creatorcontrib><creatorcontrib>CHAN, A. C</creatorcontrib><creatorcontrib>WEISS, A</creatorcontrib><creatorcontrib>EDELHOFF, S</creatorcontrib><creatorcontrib>DISTECHE, C. M</creatorcontrib><creatorcontrib>CLARK, E. A</creatorcontrib><title>Molecular cloning of human Syk. A B cell protein-tyrosine kinase associated with the surface immunoglobulin M-B cell receptor complex</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>B cell antigen receptors are multicomponent complexes consisting of the surface immunoglobulin and accessory molecules with
associating protein-tyrosine kinases. A spleen tyrosine kinase, Syk, in porcine B cells and a 72-kDa protein-tyrosine kinase,
PTK72, in murine B cells associate with the B cell antigen receptor. Herein, we report the isolation of a full-length cDNA
encoding the human homologue of Syk. This cDNA predicted a polypeptide consisting of two NH2-terminal SH2 domains and a COOH-terminal
tyrosine kinase domain. Syk is highly conserved between human and swine and is homologous to the T cell-associated protein-tyrosine
kinase ZAP-70. Both Syk mRNA and protein were detected in cells derived from multiple hematopoietic lineages. Within the B
cell compartment, Syk was expressed from pro-B cells to plasma cells. In vitro kinase assays conducted on the human Syk protein
isolated from B cells revealed the presence of autophosphorylation activity on Syk tyrosine residues. Tyrosine phosphorylation
of Syk associating with the B cell receptor complex in human was augmented rapidly after surface immunoglobulin cross-linking.
The human SYK locus was mapped to chromosome 9 at band q22.</description><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>association</subject><subject>B-Lymphocytes - enzymology</subject><subject>B-Lymphocytes - immunology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cDNA</subject><subject>Cell Line</subject><subject>cell surface</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 9</subject><subject>Cloning, Molecular - methods</subject><subject>Conserved Sequence</subject><subject>DNA, Complementary - analysis</subject><subject>Enzyme Precursors - biosynthesis</subject><subject>Enzyme Precursors - genetics</subject><subject>Enzyme Precursors - isolation & purification</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Humans</subject><subject>immunoglobulin M</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>lymphocytes B</subject><subject>man</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequence</subject><subject>Phosphorylation</subject><subject>prediction</subject><subject>protein-tyrosine kinase</subject><subject>Protein-Tyrosine Kinases - biosynthesis</subject><subject>Protein-Tyrosine Kinases - genetics</subject><subject>Protein-Tyrosine Kinases - isolation & purification</subject><subject>Receptors, Antigen, B-Cell - isolation & purification</subject><subject>Receptors, Antigen, B-Cell - metabolism</subject><subject>Restriction Mapping</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Sequence Homology, Amino Acid</subject><subject>Spleen - enzymology</subject><subject>Swine</subject><subject>Syk Kinase</subject><subject>T-Lymphocytes - immunology</subject><subject>Transfection</subject><subject>Transferases</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUQC0EKkPhEyp5gRAsUuz4lSzbipfUikVBYmc5zs3E1LEHO1GZD-C_8bTRsMQbL-65z4PQGSXnlFD5_paQmlZtLZq3VL1jtaKqEk_QhpKGVUzQH0_R5og8Ry9y_knK4y09QScNlUwwuUF_bqIHu3iTsPUxuLDFccDjMpmAb_d35_gCX2IL3uNdijO4UM37FLMLgO9cMBmwyTlaZ2bo8b2bRzyPgPOSBmMBu2laQtz62C3eBXxTrbUSWNjNsfSM087D75fo2WB8hlfrf4q-f_zw7epzdf3105eri-vKck7miqued42RnNuetVCbblBEWWh5I3hZCWquoDd9OUVn-54aI7hRAyektVwSxk7Rm8e6ZZlfC-RZTy4fJjIB4pK1klzIhrf_BalsBJV1XUDxCNpylZxg0LvkJpP2mhJ98KQfPOmDBE2VfvCkRck7Wxss3QT9MWsVU-Kv17jJ1vghmWBdPmKcCqYI_YeNbjveuwS6c9GOMOlatmVKTWtGCfsLUjmn-w</recordid><startdate>19940422</startdate><enddate>19940422</enddate><creator>CHE-LEUNG LAW</creator><creator>SIDORENKO, S. P</creator><creator>CHANDRAN, K. A</creator><creator>DRAVES, K. E</creator><creator>CHAN, A. C</creator><creator>WEISS, A</creator><creator>EDELHOFF, S</creator><creator>DISTECHE, C. M</creator><creator>CLARK, E. A</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19940422</creationdate><title>Molecular cloning of human Syk. A B cell protein-tyrosine kinase associated with the surface immunoglobulin M-B cell receptor complex</title><author>CHE-LEUNG LAW ; SIDORENKO, S. P ; CHANDRAN, K. A ; DRAVES, K. E ; CHAN, A. C ; WEISS, A ; EDELHOFF, S ; DISTECHE, C. M ; CLARK, E. A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-47d4b8a644cd39e2abf707ce94854816e247edad717bcdd1aa54a7f4009c46033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>association</topic><topic>B-Lymphocytes - enzymology</topic><topic>B-Lymphocytes - immunology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cDNA</topic><topic>Cell Line</topic><topic>cell surface</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 9</topic><topic>Cloning, Molecular - methods</topic><topic>Conserved Sequence</topic><topic>DNA, Complementary - analysis</topic><topic>Enzyme Precursors - biosynthesis</topic><topic>Enzyme Precursors - genetics</topic><topic>Enzyme Precursors - isolation & purification</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Humans</topic><topic>immunoglobulin M</topic><topic>Intracellular Signaling Peptides and Proteins</topic><topic>lymphocytes B</topic><topic>man</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequence</topic><topic>Phosphorylation</topic><topic>prediction</topic><topic>protein-tyrosine kinase</topic><topic>Protein-Tyrosine Kinases - biosynthesis</topic><topic>Protein-Tyrosine Kinases - genetics</topic><topic>Protein-Tyrosine Kinases - isolation & purification</topic><topic>Receptors, Antigen, B-Cell - isolation & purification</topic><topic>Receptors, Antigen, B-Cell - metabolism</topic><topic>Restriction Mapping</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Sequence Homology, Amino Acid</topic><topic>Spleen - enzymology</topic><topic>Swine</topic><topic>Syk Kinase</topic><topic>T-Lymphocytes - immunology</topic><topic>Transfection</topic><topic>Transferases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHE-LEUNG LAW</creatorcontrib><creatorcontrib>SIDORENKO, S. P</creatorcontrib><creatorcontrib>CHANDRAN, K. A</creatorcontrib><creatorcontrib>DRAVES, K. E</creatorcontrib><creatorcontrib>CHAN, A. C</creatorcontrib><creatorcontrib>WEISS, A</creatorcontrib><creatorcontrib>EDELHOFF, S</creatorcontrib><creatorcontrib>DISTECHE, C. M</creatorcontrib><creatorcontrib>CLARK, E. A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHE-LEUNG LAW</au><au>SIDORENKO, S. P</au><au>CHANDRAN, K. A</au><au>DRAVES, K. E</au><au>CHAN, A. C</au><au>WEISS, A</au><au>EDELHOFF, S</au><au>DISTECHE, C. M</au><au>CLARK, E. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning of human Syk. A B cell protein-tyrosine kinase associated with the surface immunoglobulin M-B cell receptor complex</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1994-04-22</date><risdate>1994</risdate><volume>269</volume><issue>16</issue><spage>12310</spage><epage>12319</epage><pages>12310-12319</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>B cell antigen receptors are multicomponent complexes consisting of the surface immunoglobulin and accessory molecules with
associating protein-tyrosine kinases. A spleen tyrosine kinase, Syk, in porcine B cells and a 72-kDa protein-tyrosine kinase,
PTK72, in murine B cells associate with the B cell antigen receptor. Herein, we report the isolation of a full-length cDNA
encoding the human homologue of Syk. This cDNA predicted a polypeptide consisting of two NH2-terminal SH2 domains and a COOH-terminal
tyrosine kinase domain. Syk is highly conserved between human and swine and is homologous to the T cell-associated protein-tyrosine
kinase ZAP-70. Both Syk mRNA and protein were detected in cells derived from multiple hematopoietic lineages. Within the B
cell compartment, Syk was expressed from pro-B cells to plasma cells. In vitro kinase assays conducted on the human Syk protein
isolated from B cells revealed the presence of autophosphorylation activity on Syk tyrosine residues. Tyrosine phosphorylation
of Syk associating with the B cell receptor complex in human was augmented rapidly after surface immunoglobulin cross-linking.
The human SYK locus was mapped to chromosome 9 at band q22.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8163536</pmid><doi>10.1016/S0021-9258(17)32717-5</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1994-04, Vol.269 (16), p.12310-12319 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76456849 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals association B-Lymphocytes - enzymology B-Lymphocytes - immunology Base Sequence Biological and medical sciences cDNA Cell Line cell surface Chromosome Mapping Chromosomes, Human, Pair 9 Cloning, Molecular - methods Conserved Sequence DNA, Complementary - analysis Enzyme Precursors - biosynthesis Enzyme Precursors - genetics Enzyme Precursors - isolation & purification Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology genes Humans immunoglobulin M Intracellular Signaling Peptides and Proteins lymphocytes B man Mice Molecular Sequence Data nucleotide sequence Phosphorylation prediction protein-tyrosine kinase Protein-Tyrosine Kinases - biosynthesis Protein-Tyrosine Kinases - genetics Protein-Tyrosine Kinases - isolation & purification Receptors, Antigen, B-Cell - isolation & purification Receptors, Antigen, B-Cell - metabolism Restriction Mapping RNA, Messenger - analysis RNA, Messenger - biosynthesis Sequence Homology, Amino Acid Spleen - enzymology Swine Syk Kinase T-Lymphocytes - immunology Transfection Transferases |
| title | Molecular cloning of human Syk. A B cell protein-tyrosine kinase associated with the surface immunoglobulin M-B cell receptor complex |
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