In-gel protein N- and C-termini identification and its application for transgenic protein characterization

A new method for the determination of N‐ and C‐termini of a protein isolated in a polyacrylamide gel is introduced. In‐gel partial protein hydrolysis by hydrochloric acid is used to generate N‐ and C‐terminal peptides for identification. This new method is complementary to existing techniques. The a...

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Veröffentlicht in:	Rapid communications in mass spectrometry 2010-12, Vol.24 (23), p.3447-3455
Hauptverfasser:	Xiang, Bosong, Macisaac, Susan, Lardizabal, Kathryn, Li, Bin
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylation Acyltransferase Alcohol dehydrogenase Amino Acid Motifs Amino Acid Sequence Animals Bovine serum albumin Diacylglycerol Diacylglycerol O-Acyltransferase - chemistry Diacylglycerol O-Acyltransferase - genetics Diacylglycerol O-Acyltransferase - metabolism Electrophoresis, Polyacrylamide Gel Glycine max - chemistry Glycine max - genetics Glycine max - metabolism Hydrochloric acid Hydrolysis Mass spectroscopy Molecular Sequence Data N-Terminus Peptide Mapping - methods Peptides - chemistry Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - chemistry Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Seeds Serum Albumin, Bovine - chemistry Soybeans Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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