Escherichia coli K12 regains its O antigen
Department of Microbiology (G08), The University of Sydney, Sydney NSW 2006, Australia ABSTRACT Summary: Extant Escherichia coli K12 strains are phenotypically rough, their lipopolysaccharide having a complete core structure, but no O antigen. We used DNA hybridization and DNA sequencing to show tha...
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| Veröffentlicht in: | Microbiology (Society for General Microbiology) 1994-01, Vol.140 (1), p.49-57 |
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| creator | Liu, Dan Reeves, Peter R |
| description | Department of Microbiology (G08), The University of Sydney, Sydney NSW 2006, Australia
ABSTRACT
Summary: Extant Escherichia coli K12 strains are phenotypically rough, their lipopolysaccharide having a complete core structure, but no O antigen. We used DNA hybridization and DNA sequencing to show that the rough phenotype of this strain is due to the presence of one of two independent mutations in the rfb gene cluster. The rfb-50 mutation, consisting of an IS5 insertion at the downstream end of rfb , is present in strain EMG2, which is representative of most K12 derivatives. The rfb-51 mutation is a deletion at the upstream end of rfb , and was found in strain WG1. A gene cloned from strain WG1 could complement the rfb-50 mutation in strain EMG2, and the complemented strain produced O antigen which was typed as O16 with cross reaction to O17.
Author for correspondence: Peter R. Reeves. Tel: +61 2 6922536. Fax: +61 2 6924571.
Keywords: Escherichia coli K12, lipopolysaccharide, O antigen, rfb gene mutations |
| doi_str_mv | 10.1099/13500872-140-1-49 |
| format | Article |
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ABSTRACT
Summary: Extant Escherichia coli K12 strains are phenotypically rough, their lipopolysaccharide having a complete core structure, but no O antigen. We used DNA hybridization and DNA sequencing to show that the rough phenotype of this strain is due to the presence of one of two independent mutations in the rfb gene cluster. The rfb-50 mutation, consisting of an IS5 insertion at the downstream end of rfb , is present in strain EMG2, which is representative of most K12 derivatives. The rfb-51 mutation is a deletion at the upstream end of rfb , and was found in strain WG1. A gene cloned from strain WG1 could complement the rfb-50 mutation in strain EMG2, and the complemented strain produced O antigen which was typed as O16 with cross reaction to O17.
Author for correspondence: Peter R. Reeves. Tel: +61 2 6922536. Fax: +61 2 6924571.
Keywords: Escherichia coli K12, lipopolysaccharide, O antigen, rfb gene mutations</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/13500872-140-1-49</identifier><identifier>PMID: 7512872</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Chromosome Mapping ; DNA Transposable Elements ; DNA, Bacterial - genetics ; Escherichia coli - genetics ; Escherichia coli - immunology ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetic Complementation Test ; Genetics ; Microbiology ; Molecular Sequence Data ; Multigene Family ; Mutation ; O Antigens ; Polysaccharides, Bacterial - genetics ; Species Specificity</subject><ispartof>Microbiology (Society for General Microbiology), 1994-01, Vol.140 (1), p.49-57</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-cecab8303d11848140fa82c52e8593cb7cf42fec650bb8cae8a867d0af522bdf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,4012,27910,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4032914$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7512872$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Dan</creatorcontrib><creatorcontrib>Reeves, Peter R</creatorcontrib><title>Escherichia coli K12 regains its O antigen</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>Department of Microbiology (G08), The University of Sydney, Sydney NSW 2006, Australia
ABSTRACT
Summary: Extant Escherichia coli K12 strains are phenotypically rough, their lipopolysaccharide having a complete core structure, but no O antigen. We used DNA hybridization and DNA sequencing to show that the rough phenotype of this strain is due to the presence of one of two independent mutations in the rfb gene cluster. The rfb-50 mutation, consisting of an IS5 insertion at the downstream end of rfb , is present in strain EMG2, which is representative of most K12 derivatives. The rfb-51 mutation is a deletion at the upstream end of rfb , and was found in strain WG1. A gene cloned from strain WG1 could complement the rfb-50 mutation in strain EMG2, and the complemented strain produced O antigen which was typed as O16 with cross reaction to O17.
Author for correspondence: Peter R. Reeves. Tel: +61 2 6922536. Fax: +61 2 6924571.
Keywords: Escherichia coli K12, lipopolysaccharide, O antigen, rfb gene mutations</description><subject>Amino Acid Sequence</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetic Complementation Test</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Mutation</subject><subject>O Antigens</subject><subject>Polysaccharides, Bacterial - genetics</subject><subject>Species Specificity</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LAzEQhoMoVas_wIOwB1EQopNsdjd7lFI_sNCLnkN2NulG9qMmLeK_N6VrTzPM-8zMy0vIFYMHBmX5yNIMQBacMgGUUVEekTMm8oxykHAc-6jTHXBKzkP4AogisAmZFBnjcXxG7ucBG-MdNk4nOLQueWc88WalXR8StwnJMtH9xq1Mf0FOrG6DuRzrlHw-zz9mr3SxfHmbPS0oCoANRYO6kimkNWNSyOjMaskx40ZmZYpVgVZwazDPoKokaiO1zIsatM04r2qbTsnt_u7aD99bEzaqcwFN2-reDNugilxkHLiIINuD6IcQvLFq7V2n_a9ioHb5qP98VHShmBJl3Lkej2-rztSHjTGQqN-Mug6oW-t1jy4cMAEpL9nu9d0ea9yq-XHeqBhQ56KRyg3RLx4e_gGXI3i8</recordid><startdate>19940101</startdate><enddate>19940101</enddate><creator>Liu, Dan</creator><creator>Reeves, Peter R</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940101</creationdate><title>Escherichia coli K12 regains its O antigen</title><author>Liu, Dan ; Reeves, Peter R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-cecab8303d11848140fa82c52e8593cb7cf42fec650bb8cae8a867d0af522bdf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>DNA Transposable Elements</topic><topic>DNA, Bacterial - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetic Complementation Test</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Mutation</topic><topic>O Antigens</topic><topic>Polysaccharides, Bacterial - genetics</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Dan</creatorcontrib><creatorcontrib>Reeves, Peter R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Dan</au><au>Reeves, Peter R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Escherichia coli K12 regains its O antigen</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>1994-01-01</date><risdate>1994</risdate><volume>140</volume><issue>1</issue><spage>49</spage><epage>57</epage><pages>49-57</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>Department of Microbiology (G08), The University of Sydney, Sydney NSW 2006, Australia
ABSTRACT
Summary: Extant Escherichia coli K12 strains are phenotypically rough, their lipopolysaccharide having a complete core structure, but no O antigen. We used DNA hybridization and DNA sequencing to show that the rough phenotype of this strain is due to the presence of one of two independent mutations in the rfb gene cluster. The rfb-50 mutation, consisting of an IS5 insertion at the downstream end of rfb , is present in strain EMG2, which is representative of most K12 derivatives. The rfb-51 mutation is a deletion at the upstream end of rfb , and was found in strain WG1. A gene cloned from strain WG1 could complement the rfb-50 mutation in strain EMG2, and the complemented strain produced O antigen which was typed as O16 with cross reaction to O17.
Author for correspondence: Peter R. Reeves. Tel: +61 2 6922536. Fax: +61 2 6924571.
Keywords: Escherichia coli K12, lipopolysaccharide, O antigen, rfb gene mutations</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>7512872</pmid><doi>10.1099/13500872-140-1-49</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; PubMed Central |
| subjects | Amino Acid Sequence Bacteriology Base Sequence Biological and medical sciences Chromosome Mapping DNA Transposable Elements DNA, Bacterial - genetics Escherichia coli - genetics Escherichia coli - immunology Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic Complementation Test Genetics Microbiology Molecular Sequence Data Multigene Family Mutation O Antigens Polysaccharides, Bacterial - genetics Species Specificity |
| title | Escherichia coli K12 regains its O antigen |
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