Effect of Serine Protease Inhibitor, N-α-Tosyl-L-lysyl-Chloromethyl Ketone (TLCK), on Cell-Mediated and Cell-Free HIV-1 Spread
The effect of a serine protease inhibitor, N-α-tosyl-L-lysyl-chloromethyl ketone (TLCK), on cell adhesion and resulting cell-to-cell HIV-1 transmission was examined. Cell-mediated vital spread was blocked by 10-4M TLCK—the nontoxic dose at which the maximum inhibition of cell adhesion was achieved....
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Veröffentlicht in: | Cellular immunology 1994-04, Vol.155 (1), p.230-236 |
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description | The effect of a serine protease inhibitor, N-α-tosyl-L-lysyl-chloromethyl ketone (TLCK), on cell adhesion and resulting cell-to-cell HIV-1 transmission was examined. Cell-mediated vital spread was blocked by 10-4M TLCK—the nontoxic dose at which the maximum inhibition of cell adhesion was achieved. TLCK (10-4-10-8M range) exhibited a similar dose-dependent effect when tested against cell-free virus infection. These findings suggest that TLCK acts by preventing both cell-cell adhesion and viral binding to the target cell. The effect of TLCK could be attributed to an irreversible modification of surface and/or intracellular proteases required for functional activation of HIV-1 envelope glycoproteins. |
doi_str_mv | 10.1006/cimm.1994.1115 |
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Cell-mediated vital spread was blocked by 10-4M TLCK—the nontoxic dose at which the maximum inhibition of cell adhesion was achieved. TLCK (10-4-10-8M range) exhibited a similar dose-dependent effect when tested against cell-free virus infection. These findings suggest that TLCK acts by preventing both cell-cell adhesion and viral binding to the target cell. 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Cell-mediated vital spread was blocked by 10-4M TLCK—the nontoxic dose at which the maximum inhibition of cell adhesion was achieved. TLCK (10-4-10-8M range) exhibited a similar dose-dependent effect when tested against cell-free virus infection. These findings suggest that TLCK acts by preventing both cell-cell adhesion and viral binding to the target cell. The effect of TLCK could be attributed to an irreversible modification of surface and/or intracellular proteases required for functional activation of HIV-1 envelope glycoproteins.</description><subject>Action of physical and chemical agents</subject><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HIV-1 - growth & development</subject><subject>human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Microbiology</subject><subject>T-Lymphocytes - microbiology</subject><subject>Tosyllysine Chloromethyl Ketone - pharmacology</subject><subject>Virology</subject><subject>Virus Replication - drug effects</subject><issn>0008-8749</issn><issn>1090-2163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9qGzEQh0VpSZ20194KOpTSQuSObK1WOpYlaUzcPxC3V6HVjrDK7sqR1gWf8kx9kT5Td7HJrfQ0MPPNMPw-Ql5xmHMA-cGFrptzrcWcc148ITMOGtiCy-VTMgMAxVQp9HNynvNPAM6FhjNyprhUXMgZebjyHt1Ao6d3mEKP9FuKA9qMdNVvQx2GmC7pF_bnN9vEfGjZmrWHqVbbNqbY4bA9tPQWhziuvtusq9v3lzT2tMK2ZZ-xCXbAhtq-OXauEyK9Wf1gnN7tEtrmBXnmbZvx5alekO_XV5vqhq2_flpVH9fMiaUemNcglFNlU0h0oJda1LpolMSSQyNEoaUVC-Clr0uvFcgCJMcauHTK66b2ywvy9nh3l-L9HvNgupDd-JLtMe6zKaUooBT8vyCXuljAcjGC8yPoUsw5oTe7FDqbDoaDmdSYSY2Z1JhJzbjw-nR5X3fYPOInF-P8zWlus7OtT7Z3IT9iApRWhRgxdcRwjOtXwGSyC9i7Mes0mjRNDP_64C-IGKf1</recordid><startdate>19940415</startdate><enddate>19940415</enddate><creator>Bourinbaiar, Aldar S.</creator><creator>Nagorny, Raisa</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19940415</creationdate><title>Effect of Serine Protease Inhibitor, N-α-Tosyl-L-lysyl-Chloromethyl Ketone (TLCK), on Cell-Mediated and Cell-Free HIV-1 Spread</title><author>Bourinbaiar, Aldar S. ; Nagorny, Raisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-f9048c87d56ec09394b95d86e710d44596a42017fb7f98065061eb016c8f9dbf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Action of physical and chemical agents</topic><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HIV-1 - growth & development</topic><topic>human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Microbiology</topic><topic>T-Lymphocytes - microbiology</topic><topic>Tosyllysine Chloromethyl Ketone - pharmacology</topic><topic>Virology</topic><topic>Virus Replication - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bourinbaiar, Aldar S.</creatorcontrib><creatorcontrib>Nagorny, Raisa</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bourinbaiar, Aldar S.</au><au>Nagorny, Raisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Serine Protease Inhibitor, N-α-Tosyl-L-lysyl-Chloromethyl Ketone (TLCK), on Cell-Mediated and Cell-Free HIV-1 Spread</atitle><jtitle>Cellular immunology</jtitle><addtitle>Cell Immunol</addtitle><date>1994-04-15</date><risdate>1994</risdate><volume>155</volume><issue>1</issue><spage>230</spage><epage>236</epage><pages>230-236</pages><issn>0008-8749</issn><eissn>1090-2163</eissn><coden>CLIMB8</coden><abstract>The effect of a serine protease inhibitor, N-α-tosyl-L-lysyl-chloromethyl ketone (TLCK), on cell adhesion and resulting cell-to-cell HIV-1 transmission was examined. 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subjects | Action of physical and chemical agents AIDS/HIV Biological and medical sciences Cell Adhesion - drug effects Dose-Response Relationship, Drug Fundamental and applied biological sciences. Psychology HIV-1 - growth & development human immunodeficiency virus 1 Humans Microbiology T-Lymphocytes - microbiology Tosyllysine Chloromethyl Ketone - pharmacology Virology Virus Replication - drug effects |
title | Effect of Serine Protease Inhibitor, N-α-Tosyl-L-lysyl-Chloromethyl Ketone (TLCK), on Cell-Mediated and Cell-Free HIV-1 Spread |
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