A Method to Increase the Sensitivity of Mutation Specific Oligonucleotide Hybridization Using Asymmetric Polymerase Chain-Reaction (PCR)

We propose a simple and reliable method to increase the sensitivity of mutation specific oligonucleotide hybridization (MSOH) at least 2.5 times, when it is used to detect mutations in samples of DNA from tumor tissues. The method is based on using single stranded (ss) DNA, amplified by asymmetric P...

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Veröffentlicht in:	Biochemical and biophysical research communications 1994-04, Vol.200 (1), p.365-369
Hauptverfasser:	Gorelov, V.N., Roher, H.D., Goretzki, P.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Codon - genetics Diverse techniques DNA Primers DNA, Neoplasm - genetics DNA, Neoplasm - isolation & purification DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics Fundamental and applied biological sciences. Psychology Humans Molecular and cellular biology Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotides, Antisense Point Mutation Polymerase Chain Reaction - methods Thyroid Neoplasms - genetics
Online-Zugang:	Volltext
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