The ATP-driven Na +-pump in the plasma membrane of the marine unicellular alga, Platymonas viridis

The ATP-supported 22NA + uptake by plasma membrane vesicles from the marine microalga, Platymonas viridis, was studied. At pH 7 in the medium, Na + uptake did not occur in the presence of ATP although ΔpH across the plasma membrane was generated. The ATP-dependent Na + uptake was induced by adding t...

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Veröffentlicht in:	FEBS letters 1994-04, Vol.343 (1), p.61-64
Hauptverfasser:	Balnokin, Yurii V., Popova, Larisa G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism AO, Acridine orange BTP, 1,3-bis-tris(hydroxymethyl)methylaminopropane Carbonyl Cyanide m-Chlorophenyl Hydrazone Cell Membrane - metabolism Chlorophyta - metabolism CICCP, m-chlorocarbonylcyanide phenylhydrazone DTT, dithiothreitol EGTA, ethylenglycol-bis(β-aminoethyl ether)N,N,N',N' tetraacetic acid HEPES, N-2-hydroxyethylpiperasine-.N-2-ethane-sulphonic acid Hydrogen-Ion Concentration Marine alga MES, 2-(nN-morpholino)ethanesulphonic acid Na +-pump Plasmalemma Platymonas viridis PM, plasma membrane PMSF, phenylmethylsulphonylfluoride Sodium - metabolism Sodium-Potassium-Exchanging ATPase - metabolism TI, trypsin inhibitor, chicken egg white purified ovomucoid Tris, tris(hydroxy-methyl)aminomethane Δs̄mH, electrochemical H+ potential difference
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