The ATP-driven Na +-pump in the plasma membrane of the marine unicellular alga, Platymonas viridis
The ATP-supported 22NA + uptake by plasma membrane vesicles from the marine microalga, Platymonas viridis, was studied. At pH 7 in the medium, Na + uptake did not occur in the presence of ATP although ΔpH across the plasma membrane was generated. The ATP-dependent Na + uptake was induced by adding t...
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| Veröffentlicht in: | FEBS letters 1994-04, Vol.343 (1), p.61-64 |
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| creator | Balnokin, Yurii V. Popova, Larisa G. |
| description | The ATP-supported
22NA
+ uptake by plasma membrane vesicles from the marine microalga,
Platymonas viridis, was studied. At pH 7 in the medium, Na
+ uptake did not occur in the presence of ATP although ΔpH across the plasma membrane was generated. The ATP-dependent Na
+ uptake was induced by adding the protonophore, CICCP. At pH 8, Na
+ uptake took place when ATP was added even without CICCP. The ΔpH generated across the plasma membrane was negligible under these conditions. The Na
+ uptake at pH 8 was not affected by CICCP and amiloride, an inhibitor of the Na
+/H
+ antiporter. It is concluded that the ATP-supported Na
+ uptake by
Pl. viridis vesicles is catalyzed by Na
+-ATPase. |
| doi_str_mv | 10.1016/0014-5793(94)80607-1 |
| format | Article |
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22NA
+ uptake by plasma membrane vesicles from the marine microalga,
Platymonas viridis, was studied. At pH 7 in the medium, Na
+ uptake did not occur in the presence of ATP although ΔpH across the plasma membrane was generated. The ATP-dependent Na
+ uptake was induced by adding the protonophore, CICCP. At pH 8, Na
+ uptake took place when ATP was added even without CICCP. The ΔpH generated across the plasma membrane was negligible under these conditions. The Na
+ uptake at pH 8 was not affected by CICCP and amiloride, an inhibitor of the Na
+/H
+ antiporter. It is concluded that the ATP-supported Na
+ uptake by
Pl. viridis vesicles is catalyzed by Na
+-ATPase.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(94)80607-1</identifier><identifier>PMID: 8163019</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Adenosine Triphosphate - metabolism ; AO, Acridine orange ; BTP, 1,3-bis-tris(hydroxymethyl)methylaminopropane ; Carbonyl Cyanide m-Chlorophenyl Hydrazone ; Cell Membrane - metabolism ; Chlorophyta - metabolism ; CICCP, m-chlorocarbonylcyanide phenylhydrazone ; DTT, dithiothreitol ; EGTA, ethylenglycol-bis(β-aminoethyl ether)N,N,N',N' tetraacetic acid ; HEPES, N-2-hydroxyethylpiperasine-.N-2-ethane-sulphonic acid ; Hydrogen-Ion Concentration ; Marine alga ; MES, 2-(nN-morpholino)ethanesulphonic acid ; Na +-pump ; Plasmalemma ; Platymonas viridis ; PM, plasma membrane ; PMSF, phenylmethylsulphonylfluoride ; Sodium - metabolism ; Sodium-Potassium-Exchanging ATPase - metabolism ; TI, trypsin inhibitor, chicken egg white purified ovomucoid ; Tris, tris(hydroxy-methyl)aminomethane ; Δs̄mH, electrochemical H+ potential difference</subject><ispartof>FEBS letters, 1994-04, Vol.343 (1), p.61-64</ispartof><rights>1994</rights><rights>FEBS Letters 343 (1994) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4861-35520c4cd3b73551f4da0d86f07dd3e85caf7e7a7269bea1c7f9a3914dda71023</citedby><cites>FETCH-LOGICAL-c4861-35520c4cd3b73551f4da0d86f07dd3e85caf7e7a7269bea1c7f9a3914dda71023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(94)80607-1$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8163019$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Balnokin, Yurii V.</creatorcontrib><creatorcontrib>Popova, Larisa G.</creatorcontrib><title>The ATP-driven Na +-pump in the plasma membrane of the marine unicellular alga, Platymonas viridis</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The ATP-supported
22NA
+ uptake by plasma membrane vesicles from the marine microalga,
Platymonas viridis, was studied. At pH 7 in the medium, Na
+ uptake did not occur in the presence of ATP although ΔpH across the plasma membrane was generated. The ATP-dependent Na
+ uptake was induced by adding the protonophore, CICCP. At pH 8, Na
+ uptake took place when ATP was added even without CICCP. The ΔpH generated across the plasma membrane was negligible under these conditions. The Na
+ uptake at pH 8 was not affected by CICCP and amiloride, an inhibitor of the Na
+/H
+ antiporter. It is concluded that the ATP-supported Na
+ uptake by
Pl. viridis vesicles is catalyzed by Na
+-ATPase.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>AO, Acridine orange</subject><subject>BTP, 1,3-bis-tris(hydroxymethyl)methylaminopropane</subject><subject>Carbonyl Cyanide m-Chlorophenyl Hydrazone</subject><subject>Cell Membrane - metabolism</subject><subject>Chlorophyta - metabolism</subject><subject>CICCP, m-chlorocarbonylcyanide phenylhydrazone</subject><subject>DTT, dithiothreitol</subject><subject>EGTA, ethylenglycol-bis(β-aminoethyl ether)N,N,N',N' tetraacetic acid</subject><subject>HEPES, N-2-hydroxyethylpiperasine-.N-2-ethane-sulphonic acid</subject><subject>Hydrogen-Ion Concentration</subject><subject>Marine alga</subject><subject>MES, 2-(nN-morpholino)ethanesulphonic acid</subject><subject>Na +-pump</subject><subject>Plasmalemma</subject><subject>Platymonas viridis</subject><subject>PM, plasma membrane</subject><subject>PMSF, phenylmethylsulphonylfluoride</subject><subject>Sodium - metabolism</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>TI, trypsin inhibitor, chicken egg white purified ovomucoid</subject><subject>Tris, tris(hydroxy-methyl)aminomethane</subject><subject>Δs̄mH, electrochemical H+ potential difference</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUE1PGzEUtCoqGmj_QZF8QkWwrR076_UFiSLSIkU0h_RsvbXfUqP9ws4G5d_jTSKOFSf7zcwbj4eQr5x954znPxjjMpspLb5peVGwnKmMfyATXiiRCZkXR2TyJvlETmJ8YmkuuD4mxwXPBeN6QsrVP6Q3q2Xmgt9gSx-AXmb90PTUt3SduL6G2ABtsCkDtEi7agc3EHyahtZbrOuhhkChfoQruqxhvW26FiLd-OCdj5_JxwrqiF8O5yn5O79b3f7OFn9-3d_eLDIri5xnYjabMiutE6VKd15JB8wVecWUcwKLmYVKoQI1zXWJwK2qNAjNpXOgOJuKU3K-9-1D9zxgXJvGxzFdit0N0ahcSiUkS0K5F9rQxRiwMn3w6UNbw5kZqzVjb2bszWhpdtUantbODv5D2aB7Wzp0mfj5nn_xNW7f5Wnmdz-nIzHiWu7Q8aHrvRGmtjYeg4nWY2vR-YB2bVzn_5_0FRwRmsI</recordid><startdate>19940418</startdate><enddate>19940418</enddate><creator>Balnokin, Yurii V.</creator><creator>Popova, Larisa G.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940418</creationdate><title>The ATP-driven Na +-pump in the plasma membrane of the marine unicellular alga, Platymonas viridis</title><author>Balnokin, Yurii V. ; Popova, Larisa G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4861-35520c4cd3b73551f4da0d86f07dd3e85caf7e7a7269bea1c7f9a3914dda71023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>AO, Acridine orange</topic><topic>BTP, 1,3-bis-tris(hydroxymethyl)methylaminopropane</topic><topic>Carbonyl Cyanide m-Chlorophenyl Hydrazone</topic><topic>Cell Membrane - metabolism</topic><topic>Chlorophyta - metabolism</topic><topic>CICCP, m-chlorocarbonylcyanide phenylhydrazone</topic><topic>DTT, dithiothreitol</topic><topic>EGTA, ethylenglycol-bis(β-aminoethyl ether)N,N,N',N' tetraacetic acid</topic><topic>HEPES, N-2-hydroxyethylpiperasine-.N-2-ethane-sulphonic acid</topic><topic>Hydrogen-Ion Concentration</topic><topic>Marine alga</topic><topic>MES, 2-(nN-morpholino)ethanesulphonic acid</topic><topic>Na +-pump</topic><topic>Plasmalemma</topic><topic>Platymonas viridis</topic><topic>PM, plasma membrane</topic><topic>PMSF, phenylmethylsulphonylfluoride</topic><topic>Sodium - metabolism</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><topic>TI, trypsin inhibitor, chicken egg white purified ovomucoid</topic><topic>Tris, tris(hydroxy-methyl)aminomethane</topic><topic>Δs̄mH, electrochemical H+ potential difference</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Balnokin, Yurii V.</creatorcontrib><creatorcontrib>Popova, Larisa G.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Balnokin, Yurii V.</au><au>Popova, Larisa G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The ATP-driven Na +-pump in the plasma membrane of the marine unicellular alga, Platymonas viridis</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1994-04-18</date><risdate>1994</risdate><volume>343</volume><issue>1</issue><spage>61</spage><epage>64</epage><pages>61-64</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The ATP-supported
22NA
+ uptake by plasma membrane vesicles from the marine microalga,
Platymonas viridis, was studied. At pH 7 in the medium, Na
+ uptake did not occur in the presence of ATP although ΔpH across the plasma membrane was generated. The ATP-dependent Na
+ uptake was induced by adding the protonophore, CICCP. At pH 8, Na
+ uptake took place when ATP was added even without CICCP. The ΔpH generated across the plasma membrane was negligible under these conditions. The Na
+ uptake at pH 8 was not affected by CICCP and amiloride, an inhibitor of the Na
+/H
+ antiporter. It is concluded that the ATP-supported Na
+ uptake by
Pl. viridis vesicles is catalyzed by Na
+-ATPase.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>8163019</pmid><doi>10.1016/0014-5793(94)80607-1</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | FEBS letters, 1994-04, Vol.343 (1), p.61-64 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76447340 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adenosine Triphosphate - metabolism AO, Acridine orange BTP, 1,3-bis-tris(hydroxymethyl)methylaminopropane Carbonyl Cyanide m-Chlorophenyl Hydrazone Cell Membrane - metabolism Chlorophyta - metabolism CICCP, m-chlorocarbonylcyanide phenylhydrazone DTT, dithiothreitol EGTA, ethylenglycol-bis(β-aminoethyl ether)N,N,N',N' tetraacetic acid HEPES, N-2-hydroxyethylpiperasine-.N-2-ethane-sulphonic acid Hydrogen-Ion Concentration Marine alga MES, 2-(nN-morpholino)ethanesulphonic acid Na +-pump Plasmalemma Platymonas viridis PM, plasma membrane PMSF, phenylmethylsulphonylfluoride Sodium - metabolism Sodium-Potassium-Exchanging ATPase - metabolism TI, trypsin inhibitor, chicken egg white purified ovomucoid Tris, tris(hydroxy-methyl)aminomethane Δs̄mH, electrochemical H+ potential difference |
| title | The ATP-driven Na +-pump in the plasma membrane of the marine unicellular alga, Platymonas viridis |
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