Zonal heterogeneity of calcium distribution in rat hepatocytes: an electron microscopic study with a combined glutaraldehyde-osmium- pyroantimonate technique
Potassium pyroantimonate in combination with osmium tetroxide is an excellent technique for investigation of the subcellular distribution of calcium ions in glutaraldehyde-fixed liver tissue. Under appropriate incubation conditions potassium ions are replaced by calcium ions to form an insoluble ele...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 1994-05, Vol.42 (5), p.593-598 |
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| container_title | The journal of histochemistry and cytochemistry |
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| creator | Angermuller, S Juchem, R Beier, K Konrad, T Kusterer, K |
| description | Potassium pyroantimonate in combination with osmium tetroxide is an excellent technique for investigation of the subcellular distribution of calcium ions in glutaraldehyde-fixed liver tissue. Under appropriate incubation conditions potassium ions are replaced by calcium ions to form an insoluble electron-dense calcium antimonate precipitate. The presence of calcium within this antimonate precipitate is confirmed by the electron spectroscopic imaging (ESI) technique. Chelator treatment with EGTA [ethylene glycol-bis-(beta-amino-ethyl ether) N',N',N'-tetraacetic acid] is usually used as a control, preventing the precipitation of calcium. In our investigation, computerized image analysis revealed a gradient of the calcium distribution from the periportal to the pericentral area. A finely dispersed precipitate was found in the mitochondrial matrix and in the euchromatin of the nuclei of the periportal hepatocytes, and in the organelles of the pericentral area a coarser deposit in lower concentration was recognized. Our findings indicate that periportal and pericentral hepatocytes retain their zonal characteristics also with respect to calcium concentration in mitochondria and nuclei. |
| doi_str_mv | 10.1177/42.5.8157930 |
| format | Article |
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Under appropriate incubation conditions potassium ions are replaced by calcium ions to form an insoluble electron-dense calcium antimonate precipitate. The presence of calcium within this antimonate precipitate is confirmed by the electron spectroscopic imaging (ESI) technique. Chelator treatment with EGTA [ethylene glycol-bis-(beta-amino-ethyl ether) N',N',N'-tetraacetic acid] is usually used as a control, preventing the precipitation of calcium. In our investigation, computerized image analysis revealed a gradient of the calcium distribution from the periportal to the pericentral area. A finely dispersed precipitate was found in the mitochondrial matrix and in the euchromatin of the nuclei of the periportal hepatocytes, and in the organelles of the pericentral area a coarser deposit in lower concentration was recognized. Our findings indicate that periportal and pericentral hepatocytes retain their zonal characteristics also with respect to calcium concentration in mitochondria and nuclei.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1177/42.5.8157930</identifier><identifier>PMID: 8157930</identifier><identifier>CODEN: JHCYAS</identifier><language>eng</language><publisher>Los Angeles, CA: Histochemical Soc</publisher><subject>Animals ; Antimony ; Biological and medical sciences ; Calcium - metabolism ; Cell Nucleus - metabolism ; Cell Nucleus - ultrastructure ; Diverse techniques ; Fundamental and applied biological sciences. Psychology ; Glutaral ; Liver - metabolism ; Liver - ultrastructure ; Male ; Microscopy, Electron ; Mitochondria, Liver - metabolism ; Mitochondria, Liver - ultrastructure ; Molecular and cellular biology ; Osmium ; Rats ; Rats, Sprague-Dawley ; Tissue Fixation - methods</subject><ispartof>The journal of histochemistry and cytochemistry, 1994-05, Vol.42 (5), p.593-598</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-ef879f397a87ac4dff3550e4d1e7f182a2b6d803dbaf08bc3fc49875c8b506da3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/42.5.8157930$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/42.5.8157930$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4066982$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8157930$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Angermuller, S</creatorcontrib><creatorcontrib>Juchem, R</creatorcontrib><creatorcontrib>Beier, K</creatorcontrib><creatorcontrib>Konrad, T</creatorcontrib><creatorcontrib>Kusterer, K</creatorcontrib><title>Zonal heterogeneity of calcium distribution in rat hepatocytes: an electron microscopic study with a combined glutaraldehyde-osmium- pyroantimonate technique</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J Histochem Cytochem</addtitle><description>Potassium pyroantimonate in combination with osmium tetroxide is an excellent technique for investigation of the subcellular distribution of calcium ions in glutaraldehyde-fixed liver tissue. Under appropriate incubation conditions potassium ions are replaced by calcium ions to form an insoluble electron-dense calcium antimonate precipitate. The presence of calcium within this antimonate precipitate is confirmed by the electron spectroscopic imaging (ESI) technique. Chelator treatment with EGTA [ethylene glycol-bis-(beta-amino-ethyl ether) N',N',N'-tetraacetic acid] is usually used as a control, preventing the precipitation of calcium. In our investigation, computerized image analysis revealed a gradient of the calcium distribution from the periportal to the pericentral area. A finely dispersed precipitate was found in the mitochondrial matrix and in the euchromatin of the nuclei of the periportal hepatocytes, and in the organelles of the pericentral area a coarser deposit in lower concentration was recognized. Our findings indicate that periportal and pericentral hepatocytes retain their zonal characteristics also with respect to calcium concentration in mitochondria and nuclei.</description><subject>Animals</subject><subject>Antimony</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Diverse techniques</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutaral</subject><subject>Liver - metabolism</subject><subject>Liver - ultrastructure</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Mitochondria, Liver - metabolism</subject><subject>Mitochondria, Liver - ultrastructure</subject><subject>Molecular and cellular biology</subject><subject>Osmium</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Tissue Fixation - methods</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10UuL1TAUAOAiynhndOdWyEIHBHtN2qQPdzL4ggE3unETTpOT2wxtU5OU0h_jfzXjLePKVSDn4zyz7AWjR8bq-h0vjuLYMFG3JX2UHZgQLBeU88fZgdKiyNMHf5pdhnBHKeNcNBfZxc4P2e-fboKB9BjRuxNOaONGnCEKBmWXkWgborfdEq2biJ2Ih5jwDNGpLWJ4T2AiOKCKPsVHq7wLys1WkRAXvZHVxp4AUW7s7ISanIYlgodBY79pzF0YU5GczJt3MEU7pmYikoiqn-yvBZ9lTwwMAZ_v71X249PH7zdf8ttvn7_efLjNFWd1zNE0dWvKtoamBsW1MaUQFLlmWBvWFFB0lW5oqTswtOlUaRRvm1qophO00lBeZdfnvLN3qWyIcrRB4TDAhG4Jsq4455S1Cb49w_tBg0cjZ29H8JtkVN5fQ_JCCrmvN_GXe96lG1E_4H_xV3scQtq48TApGx4Yp1XVNkVib84swAnlnVt8uln4X8nXZ9vbU79ajzKMMAypASbXdf1rRVuWfwBbNbBR</recordid><startdate>19940501</startdate><enddate>19940501</enddate><creator>Angermuller, S</creator><creator>Juchem, R</creator><creator>Beier, K</creator><creator>Konrad, T</creator><creator>Kusterer, K</creator><general>Histochemical Soc</general><general>SAGE Publications</general><general>Histochemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940501</creationdate><title>Zonal heterogeneity of calcium distribution in rat hepatocytes: an electron microscopic study with a combined glutaraldehyde-osmium- pyroantimonate technique</title><author>Angermuller, S ; Juchem, R ; Beier, K ; Konrad, T ; Kusterer, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-ef879f397a87ac4dff3550e4d1e7f182a2b6d803dbaf08bc3fc49875c8b506da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Antimony</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Diverse techniques</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutaral</topic><topic>Liver - metabolism</topic><topic>Liver - ultrastructure</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>Mitochondria, Liver - metabolism</topic><topic>Mitochondria, Liver - ultrastructure</topic><topic>Molecular and cellular biology</topic><topic>Osmium</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Tissue Fixation - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Angermuller, S</creatorcontrib><creatorcontrib>Juchem, R</creatorcontrib><creatorcontrib>Beier, K</creatorcontrib><creatorcontrib>Konrad, T</creatorcontrib><creatorcontrib>Kusterer, K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Angermuller, S</au><au>Juchem, R</au><au>Beier, K</au><au>Konrad, T</au><au>Kusterer, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Zonal heterogeneity of calcium distribution in rat hepatocytes: an electron microscopic study with a combined glutaraldehyde-osmium- pyroantimonate technique</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>1994-05-01</date><risdate>1994</risdate><volume>42</volume><issue>5</issue><spage>593</spage><epage>598</epage><pages>593-598</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><coden>JHCYAS</coden><abstract>Potassium pyroantimonate in combination with osmium tetroxide is an excellent technique for investigation of the subcellular distribution of calcium ions in glutaraldehyde-fixed liver tissue. Under appropriate incubation conditions potassium ions are replaced by calcium ions to form an insoluble electron-dense calcium antimonate precipitate. The presence of calcium within this antimonate precipitate is confirmed by the electron spectroscopic imaging (ESI) technique. Chelator treatment with EGTA [ethylene glycol-bis-(beta-amino-ethyl ether) N',N',N'-tetraacetic acid] is usually used as a control, preventing the precipitation of calcium. In our investigation, computerized image analysis revealed a gradient of the calcium distribution from the periportal to the pericentral area. A finely dispersed precipitate was found in the mitochondrial matrix and in the euchromatin of the nuclei of the periportal hepatocytes, and in the organelles of the pericentral area a coarser deposit in lower concentration was recognized. Our findings indicate that periportal and pericentral hepatocytes retain their zonal characteristics also with respect to calcium concentration in mitochondria and nuclei.</abstract><cop>Los Angeles, CA</cop><pub>Histochemical Soc</pub><pmid>8157930</pmid><doi>10.1177/42.5.8157930</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antimony Biological and medical sciences Calcium - metabolism Cell Nucleus - metabolism Cell Nucleus - ultrastructure Diverse techniques Fundamental and applied biological sciences. Psychology Glutaral Liver - metabolism Liver - ultrastructure Male Microscopy, Electron Mitochondria, Liver - metabolism Mitochondria, Liver - ultrastructure Molecular and cellular biology Osmium Rats Rats, Sprague-Dawley Tissue Fixation - methods |
| title | Zonal heterogeneity of calcium distribution in rat hepatocytes: an electron microscopic study with a combined glutaraldehyde-osmium- pyroantimonate technique |
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