An adhesion assay using minimal shear force to remove nonadherent cells
A new 96-well microtiter plate based adhesion assay was developed to measure weak cell adhesion. This assay is distinct from other adhesion assays by the procedure in which the nonadherent cells are removed. In most conventional adhesion assays, nonadherent cells are removed by aspiration followed b...
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| Veröffentlicht in: | Journal of immunological methods 1994-04, Vol.170 (2), p.159-166 |
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| container_title | Journal of immunological methods |
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| creator | St. John, Joni J. Schroen, Daniel J. Tak Cheung, H. |
| description | A new 96-well microtiter plate based adhesion assay was developed to measure weak cell adhesion. This assay is distinct from other adhesion assays by the procedure in which the nonadherent cells are removed. In most conventional adhesion assays, nonadherent cells are removed by aspiration followed by repeated washes. However, the shear force generated by such washing also detaches weakly adherent cells. In the minimal shear force adhesion assay (MSFA) described here, the removal of nonadherent cells is carried out by applying a gentle shear force in a fluid environment. In this procedure, adherent cells are not subjected to harsh and variable washing forces and are not exposed to surface tension caused by the removal of washing fluid between successive washes. Using the lymphoid cell lines XC1.5/51 and MPC11, the number of adherent cells determined by this new adhesion assay is three times higher than the conventional adhesion assay. This MSFA assay is simple, consistent, and easy to perform. With modifications for applying a defined shear force, this assay can be adopted to compare cell adhesion strength to various substrata. |
| doi_str_mv | 10.1016/0022-1759(94)90391-3 |
| format | Article |
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This assay is distinct from other adhesion assays by the procedure in which the nonadherent cells are removed. In most conventional adhesion assays, nonadherent cells are removed by aspiration followed by repeated washes. However, the shear force generated by such washing also detaches weakly adherent cells. In the minimal shear force adhesion assay (MSFA) described here, the removal of nonadherent cells is carried out by applying a gentle shear force in a fluid environment. In this procedure, adherent cells are not subjected to harsh and variable washing forces and are not exposed to surface tension caused by the removal of washing fluid between successive washes. Using the lymphoid cell lines XC1.5/51 and MPC11, the number of adherent cells determined by this new adhesion assay is three times higher than the conventional adhesion assay. This MSFA assay is simple, consistent, and easy to perform. 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Psychology ; Fundamental immunology ; Gelatin - metabolism ; Laminin - metabolism ; Lymphocyte ; Lymphocytes - cytology ; Lymphocytes - metabolism ; Mice ; Molecular immunology ; Multiple Myeloma - pathology ; Sensitivity and Specificity ; Serum Albumin, Bovine - metabolism ; Techniques ; Tumor Cells, Cultured ; Weak adhesion</subject><ispartof>Journal of immunological methods, 1994-04, Vol.170 (2), p.159-166</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-59169ce1eaa28f420676b9c05b958c0ede476f00f5773576c3d60204849fd46d3</citedby><cites>FETCH-LOGICAL-c386t-59169ce1eaa28f420676b9c05b958c0ede476f00f5773576c3d60204849fd46d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-1759(94)90391-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4040798$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8157994$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>St. John, Joni J.</creatorcontrib><creatorcontrib>Schroen, Daniel J.</creatorcontrib><creatorcontrib>Tak Cheung, H.</creatorcontrib><title>An adhesion assay using minimal shear force to remove nonadherent cells</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>A new 96-well microtiter plate based adhesion assay was developed to measure weak cell adhesion. This assay is distinct from other adhesion assays by the procedure in which the nonadherent cells are removed. In most conventional adhesion assays, nonadherent cells are removed by aspiration followed by repeated washes. However, the shear force generated by such washing also detaches weakly adherent cells. In the minimal shear force adhesion assay (MSFA) described here, the removal of nonadherent cells is carried out by applying a gentle shear force in a fluid environment. In this procedure, adherent cells are not subjected to harsh and variable washing forces and are not exposed to surface tension caused by the removal of washing fluid between successive washes. Using the lymphoid cell lines XC1.5/51 and MPC11, the number of adherent cells determined by this new adhesion assay is three times higher than the conventional adhesion assay. This MSFA assay is simple, consistent, and easy to perform. With modifications for applying a defined shear force, this assay can be adopted to compare cell adhesion strength to various substrata.</description><subject>Adhesion assay</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion</subject><subject>Cell Separation - methods</subject><subject>Extracellular Matrix - metabolism</subject><subject>Fibronectins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gelatin - metabolism</subject><subject>Laminin - metabolism</subject><subject>Lymphocyte</subject><subject>Lymphocytes - cytology</subject><subject>Lymphocytes - metabolism</subject><subject>Mice</subject><subject>Molecular immunology</subject><subject>Multiple Myeloma - pathology</subject><subject>Sensitivity and Specificity</subject><subject>Serum Albumin, Bovine - metabolism</subject><subject>Techniques</subject><subject>Tumor Cells, Cultured</subject><subject>Weak adhesion</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVoSDcf_6AFHUppD05GtixZl0AIzQcEcknOQiuPuyq2tNV4F_bf184ue-xpBuZ5h5mHsS8CrgUIdQNQloXQtflh5E8DlRFFdcIWotFloQ3Un9jiiHxm50R_AECAgjN21ohaGyMX7PEucteukEKaGiK34xsK8TcfQgyD6zmt0GXepeyRj4lnHNIWeUxxTmWMI_fY93TJTjvXE14d6gV7f_j1dv9UvLw-Pt_fvRS-atRY1EYo41Ggc2XTyRKUVkvjoV6auvGALUqtOoCu1rqqtfJVq6AE2UjTtVK11QX7vt-7zunvBmm0Q6D5AhcxbchqJaUwpZhAuQd9TkQZO7vO00N5ZwXY2Z-d5dhZjjXSfviz1RT7eti_WQ7YHkMHYdP822HuyLu-yy76QEdMggRtmgm73WM4udgGzJZ8wOixDRn9aNsU_n_HP4Kyip0</recordid><startdate>19940415</startdate><enddate>19940415</enddate><creator>St. John, Joni J.</creator><creator>Schroen, Daniel J.</creator><creator>Tak Cheung, H.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940415</creationdate><title>An adhesion assay using minimal shear force to remove nonadherent cells</title><author>St. John, Joni J. ; Schroen, Daniel J. ; Tak Cheung, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-59169ce1eaa28f420676b9c05b958c0ede476f00f5773576c3d60204849fd46d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adhesion assay</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion</topic><topic>Cell Separation - methods</topic><topic>Extracellular Matrix - metabolism</topic><topic>Fibronectins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gelatin - metabolism</topic><topic>Laminin - metabolism</topic><topic>Lymphocyte</topic><topic>Lymphocytes - cytology</topic><topic>Lymphocytes - metabolism</topic><topic>Mice</topic><topic>Molecular immunology</topic><topic>Multiple Myeloma - pathology</topic><topic>Sensitivity and Specificity</topic><topic>Serum Albumin, Bovine - metabolism</topic><topic>Techniques</topic><topic>Tumor Cells, Cultured</topic><topic>Weak adhesion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>St. John, Joni J.</creatorcontrib><creatorcontrib>Schroen, Daniel J.</creatorcontrib><creatorcontrib>Tak Cheung, H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>St. John, Joni J.</au><au>Schroen, Daniel J.</au><au>Tak Cheung, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An adhesion assay using minimal shear force to remove nonadherent cells</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1994-04-15</date><risdate>1994</risdate><volume>170</volume><issue>2</issue><spage>159</spage><epage>166</epage><pages>159-166</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>A new 96-well microtiter plate based adhesion assay was developed to measure weak cell adhesion. This assay is distinct from other adhesion assays by the procedure in which the nonadherent cells are removed. In most conventional adhesion assays, nonadherent cells are removed by aspiration followed by repeated washes. However, the shear force generated by such washing also detaches weakly adherent cells. In the minimal shear force adhesion assay (MSFA) described here, the removal of nonadherent cells is carried out by applying a gentle shear force in a fluid environment. In this procedure, adherent cells are not subjected to harsh and variable washing forces and are not exposed to surface tension caused by the removal of washing fluid between successive washes. Using the lymphoid cell lines XC1.5/51 and MPC11, the number of adherent cells determined by this new adhesion assay is three times higher than the conventional adhesion assay. This MSFA assay is simple, consistent, and easy to perform. With modifications for applying a defined shear force, this assay can be adopted to compare cell adhesion strength to various substrata.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8157994</pmid><doi>10.1016/0022-1759(94)90391-3</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1994-04, Vol.170 (2), p.159-166 |
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| subjects | Adhesion assay Animals Biological and medical sciences Cell Adhesion Cell Separation - methods Extracellular Matrix - metabolism Fibronectins - metabolism Fundamental and applied biological sciences. Psychology Fundamental immunology Gelatin - metabolism Laminin - metabolism Lymphocyte Lymphocytes - cytology Lymphocytes - metabolism Mice Molecular immunology Multiple Myeloma - pathology Sensitivity and Specificity Serum Albumin, Bovine - metabolism Techniques Tumor Cells, Cultured Weak adhesion |
| title | An adhesion assay using minimal shear force to remove nonadherent cells |
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