Isolation and characterization of a 14500 molecular weight protein from brains and tissue cultures persistently infected with Borna disease virus
Institut für Virologie, Freie Universität Berlin and 1 Robert Koch-Institut des Bundesgesundheitsamtes, Nordufer 20, 1000 Berlin 65, F.R.G. A protein with an apparent molecular weight of 14 500 (14.5K) was extractable from homogenates of Borna disease virus-infected brains and tissue cultures using...
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| Veröffentlicht in: | Journal of general virology 1985-11, Vol.66 (11), p.2479-2484 |
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| container_title | Journal of general virology |
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| creator | Schadler, R Diringer, H Ludwig, H |
| description | Institut für Virologie, Freie Universität Berlin
and 1 Robert Koch-Institut des Bundesgesundheitsamtes, Nordufer 20, 1000 Berlin 65, F.R.G.
A protein with an apparent molecular weight of 14 500 (14.5K) was extractable from homogenates of Borna disease virus-infected brains and tissue cultures using high concentrations of detergent and salt and by differential centrifugation procedures. The protein, present in an aggregated form, was remarkably resistant to proteinase K. Specific antibodies prepared in the homologous system (rat) recognized the 14.5K protein from various sources (infected brain of rat, mouse or chicken, and tissue cultures), but did not neutralize infectivity nor stain Borna disease virus-specific antigens from in vitro or in vivo preparations. Post-infection immune sera from different animal species did not detect the protein. This 14.5K protein was infection-specific but not disease-specific, and is inferred to be part of an internal virion component.
Keywords: BD virus, 14.5K protein, persistent infection
Received 8 July 1985;
accepted 9 August 1985. |
| doi_str_mv | 10.1099/0022-1317-66-11-2479 |
| format | Article |
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and 1 Robert Koch-Institut des Bundesgesundheitsamtes, Nordufer 20, 1000 Berlin 65, F.R.G.
A protein with an apparent molecular weight of 14 500 (14.5K) was extractable from homogenates of Borna disease virus-infected brains and tissue cultures using high concentrations of detergent and salt and by differential centrifugation procedures. The protein, present in an aggregated form, was remarkably resistant to proteinase K. Specific antibodies prepared in the homologous system (rat) recognized the 14.5K protein from various sources (infected brain of rat, mouse or chicken, and tissue cultures), but did not neutralize infectivity nor stain Borna disease virus-specific antigens from in vitro or in vivo preparations. Post-infection immune sera from different animal species did not detect the protein. This 14.5K protein was infection-specific but not disease-specific, and is inferred to be part of an internal virion component.
Keywords: BD virus, 14.5K protein, persistent infection
Received 8 July 1985;
accepted 9 August 1985.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-66-11-2479</identifier><identifier>PMID: 3932595</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>animal proteins ; Animals ; Borna Disease - immunology ; Borna Disease - microbiology ; Borna disease virus ; Brain - microbiology ; Brain Chemistry ; Cells, Cultured ; characterization ; Chickens ; Horse Diseases - microbiology ; Horses ; Humans ; Immune Sera - immunology ; isolation ; Mice ; Mice, Inbred BALB C ; Molecular Weight ; Rabbits ; Rats ; Rats, Inbred Strains ; Viral Proteins - analysis ; Viral Proteins - immunology ; Viral Proteins - isolation & purification ; Viruses, Unclassified</subject><ispartof>Journal of general virology, 1985-11, Vol.66 (11), p.2479-2484</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-c5e028955cee1c7d055329753cc9f9aa52d2f3a18293d317622970562545f4953</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,3733,3734,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3932595$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schadler, R</creatorcontrib><creatorcontrib>Diringer, H</creatorcontrib><creatorcontrib>Ludwig, H</creatorcontrib><title>Isolation and characterization of a 14500 molecular weight protein from brains and tissue cultures persistently infected with Borna disease virus</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Institut für Virologie, Freie Universität Berlin
and 1 Robert Koch-Institut des Bundesgesundheitsamtes, Nordufer 20, 1000 Berlin 65, F.R.G.
A protein with an apparent molecular weight of 14 500 (14.5K) was extractable from homogenates of Borna disease virus-infected brains and tissue cultures using high concentrations of detergent and salt and by differential centrifugation procedures. The protein, present in an aggregated form, was remarkably resistant to proteinase K. Specific antibodies prepared in the homologous system (rat) recognized the 14.5K protein from various sources (infected brain of rat, mouse or chicken, and tissue cultures), but did not neutralize infectivity nor stain Borna disease virus-specific antigens from in vitro or in vivo preparations. Post-infection immune sera from different animal species did not detect the protein. This 14.5K protein was infection-specific but not disease-specific, and is inferred to be part of an internal virion component.
Keywords: BD virus, 14.5K protein, persistent infection
Received 8 July 1985;
accepted 9 August 1985.</description><subject>animal proteins</subject><subject>Animals</subject><subject>Borna Disease - immunology</subject><subject>Borna Disease - microbiology</subject><subject>Borna disease virus</subject><subject>Brain - microbiology</subject><subject>Brain Chemistry</subject><subject>Cells, Cultured</subject><subject>characterization</subject><subject>Chickens</subject><subject>Horse Diseases - microbiology</subject><subject>Horses</subject><subject>Humans</subject><subject>Immune Sera - immunology</subject><subject>isolation</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Weight</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Viral Proteins - analysis</subject><subject>Viral Proteins - immunology</subject><subject>Viral Proteins - isolation & purification</subject><subject>Viruses, Unclassified</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc2KFDEUhYMoYzv6BopZCS5K81-dpTP4MzDgQmcd0qlb3ZGqSpubshnfwjc2ZTUDrgI53z2Xew4hLzl7x5m17xkTouGSt40xDeeNUK19RDZcGd2ICjwmmwfkKXmG-IMxrpRuL8iFtFJoqzfkzw2mwZeYJuqnjoaDzz4UyPH3-pl66ilXmjE6pgHCPPhMTxD3h0KPORWIE-1zGuku-zjhP5MSEWeglS1zBqRHyBixwFSGexqnHuqCjp5iOdCrlCdPu4jgEeivmGd8Tp70fkB4cX4vyd2nj9-vvzS3Xz_fXH-4bYJipjRBAxNbq3UA4KHtmNZS2FbLEGxvvdeiE730fCus7GoCRlSVaSO00r2yWl6SN6tvPePnDFjcGDHAMPgJ0oyuNUpqxbYVVCsYckLM0LtjjqPP944ztzThlpjdErMzxnHulibq2Kuz_7wboXsYOkdf9berfqhhnmIGt4dpjHXJLiZXo_jP6_XK9j45v88R3d03wbhcChVCS_kXhuKc1g</recordid><startdate>198511</startdate><enddate>198511</enddate><creator>Schadler, R</creator><creator>Diringer, H</creator><creator>Ludwig, H</creator><general>Soc General Microbiol</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198511</creationdate><title>Isolation and characterization of a 14500 molecular weight protein from brains and tissue cultures persistently infected with Borna disease virus</title><author>Schadler, R ; Diringer, H ; Ludwig, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-c5e028955cee1c7d055329753cc9f9aa52d2f3a18293d317622970562545f4953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>animal proteins</topic><topic>Animals</topic><topic>Borna Disease - immunology</topic><topic>Borna Disease - microbiology</topic><topic>Borna disease virus</topic><topic>Brain - microbiology</topic><topic>Brain Chemistry</topic><topic>Cells, Cultured</topic><topic>characterization</topic><topic>Chickens</topic><topic>Horse Diseases - microbiology</topic><topic>Horses</topic><topic>Humans</topic><topic>Immune Sera - immunology</topic><topic>isolation</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Weight</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Viral Proteins - analysis</topic><topic>Viral Proteins - immunology</topic><topic>Viral Proteins - isolation & purification</topic><topic>Viruses, Unclassified</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schadler, R</creatorcontrib><creatorcontrib>Diringer, H</creatorcontrib><creatorcontrib>Ludwig, H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schadler, R</au><au>Diringer, H</au><au>Ludwig, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of a 14500 molecular weight protein from brains and tissue cultures persistently infected with Borna disease virus</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1985-11</date><risdate>1985</risdate><volume>66</volume><issue>11</issue><spage>2479</spage><epage>2484</epage><pages>2479-2484</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Institut für Virologie, Freie Universität Berlin
and 1 Robert Koch-Institut des Bundesgesundheitsamtes, Nordufer 20, 1000 Berlin 65, F.R.G.
A protein with an apparent molecular weight of 14 500 (14.5K) was extractable from homogenates of Borna disease virus-infected brains and tissue cultures using high concentrations of detergent and salt and by differential centrifugation procedures. The protein, present in an aggregated form, was remarkably resistant to proteinase K. Specific antibodies prepared in the homologous system (rat) recognized the 14.5K protein from various sources (infected brain of rat, mouse or chicken, and tissue cultures), but did not neutralize infectivity nor stain Borna disease virus-specific antigens from in vitro or in vivo preparations. Post-infection immune sera from different animal species did not detect the protein. This 14.5K protein was infection-specific but not disease-specific, and is inferred to be part of an internal virion component.
Keywords: BD virus, 14.5K protein, persistent infection
Received 8 July 1985;
accepted 9 August 1985.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>3932595</pmid><doi>10.1099/0022-1317-66-11-2479</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1985-11, Vol.66 (11), p.2479-2484 |
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| language | eng |
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| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | animal proteins Animals Borna Disease - immunology Borna Disease - microbiology Borna disease virus Brain - microbiology Brain Chemistry Cells, Cultured characterization Chickens Horse Diseases - microbiology Horses Humans Immune Sera - immunology isolation Mice Mice, Inbred BALB C Molecular Weight Rabbits Rats Rats, Inbred Strains Viral Proteins - analysis Viral Proteins - immunology Viral Proteins - isolation & purification Viruses, Unclassified |
| title | Isolation and characterization of a 14500 molecular weight protein from brains and tissue cultures persistently infected with Borna disease virus |
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