Tadpole erythrocytes: Luminescent properties with dark field microscopy

To characterize and classify erythrocytes of ranid tadpoles, alcohol‐fixed blood smears were studied with dark field illumination. All preclimax stage (limb bud‐foot) Rana castesbeiana tadpoles from ponds in Massachusetts had red blood cells that were polymorphic. The majority of cells (88%) showed...

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Veröffentlicht in:Journal of morphology (1931) 1985-06, Vol.184 (3), p.343-350
Hauptverfasser: Tyler, Lisa W., Piotrowski, Deborah C., Kaltenbach, Jane C.
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Piotrowski, Deborah C.
Kaltenbach, Jane C.
description To characterize and classify erythrocytes of ranid tadpoles, alcohol‐fixed blood smears were studied with dark field illumination. All preclimax stage (limb bud‐foot) Rana castesbeiana tadpoles from ponds in Massachusetts had red blood cells that were polymorphic. The majority of cells (88%) showed a bright, granular luminescence varying from white to blue‐grey, whereas, cytoplasm of the other cells was smooth, black, and nonluminescent. On the other hand, tadpoles in similar stages from other species (Rana clamitans, Mass. and Rana pipens, Vermont) and from R. catesbeiana tadpoles from other locations (Wisconsin and North Carolina) had no observable cytoplasmic luminescence in any of their red blood cells. Moreover, as Mass. R. catesbeiana underwent metamorphic climax their luminescent cells disappeared and were replaced by small, round, dark, nonluminescent cells, precursors of the oval, nonluminescent erythrocytes characteristic of adult frogs. Cells with black nonluminescent cytoplasm generally contained nuclei which were luminescent. In conclusion, two main types of red blood cells‐those with and those without cytoplasmic luminescence‐are distinguishable with dark field microscopy. Luminescence of the cells varies with species, geographic location, and developmental stage of the tadpoles.
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Morphol</addtitle><description>To characterize and classify erythrocytes of ranid tadpoles, alcohol‐fixed blood smears were studied with dark field illumination. All preclimax stage (limb bud‐foot) Rana castesbeiana tadpoles from ponds in Massachusetts had red blood cells that were polymorphic. The majority of cells (88%) showed a bright, granular luminescence varying from white to blue‐grey, whereas, cytoplasm of the other cells was smooth, black, and nonluminescent. On the other hand, tadpoles in similar stages from other species (Rana clamitans, Mass. and Rana pipens, Vermont) and from R. catesbeiana tadpoles from other locations (Wisconsin and North Carolina) had no observable cytoplasmic luminescence in any of their red blood cells. Moreover, as Mass. R. catesbeiana underwent metamorphic climax their luminescent cells disappeared and were replaced by small, round, dark, nonluminescent cells, precursors of the oval, nonluminescent erythrocytes characteristic of adult frogs. Cells with black nonluminescent cytoplasm generally contained nuclei which were luminescent. In conclusion, two main types of red blood cells‐those with and those without cytoplasmic luminescence‐are distinguishable with dark field microscopy. Luminescence of the cells varies with species, geographic location, and developmental stage of the tadpoles.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell morphology</subject><subject>Erythrocytes - cytology</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Luminescent Measurements</topic><topic>Metamorphosis, Biological</topic><topic>Molecular and cellular biology</topic><topic>Rana catesbeiana</topic><topic>Rana pipiens</topic><topic>Ranidae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tyler, Lisa W.</creatorcontrib><creatorcontrib>Piotrowski, Deborah C.</creatorcontrib><creatorcontrib>Kaltenbach, Jane C.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of morphology (1931)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tyler, Lisa W.</au><au>Piotrowski, Deborah C.</au><au>Kaltenbach, Jane C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tadpole erythrocytes: Luminescent properties with dark field microscopy</atitle><jtitle>Journal of morphology (1931)</jtitle><addtitle>J. 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subjects Animals
Biological and medical sciences
Cell morphology
Erythrocytes - cytology
Fundamental and applied biological sciences. Psychology
Luminescent Measurements
Metamorphosis, Biological
Molecular and cellular biology
Rana catesbeiana
Rana pipiens
Ranidae
title Tadpole erythrocytes: Luminescent properties with dark field microscopy
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