Traumatic injury induces interleukin-6 production by human astrocytes
The brain is being evaluated as a de novo source of cytokines. Because recent evidence indicates that interleukin-6 (IL-6) may influence blood-brain barrier function and vascular permeability, we have sought to determine whether mechanical injury can directly induce in situ cerebral IL-6 production....
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| Veröffentlicht in: | Brain research 1994-02, Vol.636 (1), p.139-142 |
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| container_title | Brain research |
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| creator | Hariri, Robert J. Chang, Victor A. Barie, Philip S. Wang, Ruby S. Sharif, Setareh F. Ghajar, Jam B.G. |
| description | The brain is being evaluated as a de novo source of cytokines. Because recent evidence indicates that interleukin-6 (IL-6) may influence blood-brain barrier function and vascular permeability, we have sought to determine whether mechanical injury can directly induce in situ cerebral IL-6 production. Adult human astrocyte cultures were subjected to mechanical injury by the in vitro method of fluid percussion barotrauma, developed in our laboratory. Serial supernatant samples were collected for 8 h and evaluated for IL-6 activity using a proliferation assay employing the dependent B cell hybridoma cell line, B9. At optimum injury, the IL-6 level became significantly (
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| doi_str_mv | 10.1016/0006-8993(94)90188-0 |
| format | Article |
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P<0.0001, analysis of variance) elevated from baseline 2 h after trauma and continued to increase over the observation period. Our study shows that following mechanical injury human astrocytes produce IL-6, which may contribute to post-traumatic cerebrovascular dysfunction. Elucidating the precise role of intracerebral cytokines is essential to our understanding of the mechanism responsible for post-traumatic cerebrovascular dysfunction.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(94)90188-0</identifier><identifier>PMID: 8156401</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Astrocytes - metabolism ; Atmospheric Pressure ; Biological and medical sciences ; Brain Injuries - metabolism ; Cell Division - physiology ; Cell Line ; Cytokine ; Human astrocyte culture ; Humans ; Injuries of the nervous system and the skull. Diseases due to physical agents ; Interleukin-6 ; Interleukin-6 - biosynthesis ; Medical sciences ; Trauma ; Traumas. Diseases due to physical agents</subject><ispartof>Brain research, 1994-02, Vol.636 (1), p.139-142</ispartof><rights>1994 Elsevier Science B.V. All rights reserved</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-4431cbc044bdbee01eab3745e5b6803e4a4c0c226176916c46abf8ce6a9a19bc3</citedby><cites>FETCH-LOGICAL-c417t-4431cbc044bdbee01eab3745e5b6803e4a4c0c226176916c46abf8ce6a9a19bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006899394901880$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3891501$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8156401$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hariri, Robert J.</creatorcontrib><creatorcontrib>Chang, Victor A.</creatorcontrib><creatorcontrib>Barie, Philip S.</creatorcontrib><creatorcontrib>Wang, Ruby S.</creatorcontrib><creatorcontrib>Sharif, Setareh F.</creatorcontrib><creatorcontrib>Ghajar, Jam B.G.</creatorcontrib><title>Traumatic injury induces interleukin-6 production by human astrocytes</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The brain is being evaluated as a de novo source of cytokines. Because recent evidence indicates that interleukin-6 (IL-6) may influence blood-brain barrier function and vascular permeability, we have sought to determine whether mechanical injury can directly induce in situ cerebral IL-6 production. Adult human astrocyte cultures were subjected to mechanical injury by the in vitro method of fluid percussion barotrauma, developed in our laboratory. Serial supernatant samples were collected for 8 h and evaluated for IL-6 activity using a proliferation assay employing the dependent B cell hybridoma cell line, B9. At optimum injury, the IL-6 level became significantly (
P<0.0001, analysis of variance) elevated from baseline 2 h after trauma and continued to increase over the observation period. Our study shows that following mechanical injury human astrocytes produce IL-6, which may contribute to post-traumatic cerebrovascular dysfunction. Elucidating the precise role of intracerebral cytokines is essential to our understanding of the mechanism responsible for post-traumatic cerebrovascular dysfunction.</description><subject>Astrocytes - metabolism</subject><subject>Atmospheric Pressure</subject><subject>Biological and medical sciences</subject><subject>Brain Injuries - metabolism</subject><subject>Cell Division - physiology</subject><subject>Cell Line</subject><subject>Cytokine</subject><subject>Human astrocyte culture</subject><subject>Humans</subject><subject>Injuries of the nervous system and the skull. Diseases due to physical agents</subject><subject>Interleukin-6</subject><subject>Interleukin-6 - biosynthesis</subject><subject>Medical sciences</subject><subject>Trauma</subject><subject>Traumas. 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Diseases due to physical agents</topic><topic>Interleukin-6</topic><topic>Interleukin-6 - biosynthesis</topic><topic>Medical sciences</topic><topic>Trauma</topic><topic>Traumas. Diseases due to physical agents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hariri, Robert J.</creatorcontrib><creatorcontrib>Chang, Victor A.</creatorcontrib><creatorcontrib>Barie, Philip S.</creatorcontrib><creatorcontrib>Wang, Ruby S.</creatorcontrib><creatorcontrib>Sharif, Setareh F.</creatorcontrib><creatorcontrib>Ghajar, Jam B.G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hariri, Robert J.</au><au>Chang, Victor A.</au><au>Barie, Philip S.</au><au>Wang, Ruby S.</au><au>Sharif, Setareh F.</au><au>Ghajar, Jam B.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Traumatic injury induces interleukin-6 production by human astrocytes</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1994-02-04</date><risdate>1994</risdate><volume>636</volume><issue>1</issue><spage>139</spage><epage>142</epage><pages>139-142</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The brain is being evaluated as a de novo source of cytokines. Because recent evidence indicates that interleukin-6 (IL-6) may influence blood-brain barrier function and vascular permeability, we have sought to determine whether mechanical injury can directly induce in situ cerebral IL-6 production. Adult human astrocyte cultures were subjected to mechanical injury by the in vitro method of fluid percussion barotrauma, developed in our laboratory. Serial supernatant samples were collected for 8 h and evaluated for IL-6 activity using a proliferation assay employing the dependent B cell hybridoma cell line, B9. At optimum injury, the IL-6 level became significantly (
P<0.0001, analysis of variance) elevated from baseline 2 h after trauma and continued to increase over the observation period. Our study shows that following mechanical injury human astrocytes produce IL-6, which may contribute to post-traumatic cerebrovascular dysfunction. Elucidating the precise role of intracerebral cytokines is essential to our understanding of the mechanism responsible for post-traumatic cerebrovascular dysfunction.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>8156401</pmid><doi>10.1016/0006-8993(94)90188-0</doi><tpages>4</tpages></addata></record> |
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| subjects | Astrocytes - metabolism Atmospheric Pressure Biological and medical sciences Brain Injuries - metabolism Cell Division - physiology Cell Line Cytokine Human astrocyte culture Humans Injuries of the nervous system and the skull. Diseases due to physical agents Interleukin-6 Interleukin-6 - biosynthesis Medical sciences Trauma Traumas. Diseases due to physical agents |
| title | Traumatic injury induces interleukin-6 production by human astrocytes |
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