Radioimmunoassay of IgG and IgM anti-albumin autoantibodies in human serum
An antigen-coated plate radioimmunoassay used to detect autoantibodies of IgG and IgM classes against human serum albumin is described. It comprises 3 steps: (a) adsorption of glutaraldehyde-polymerized human serum albumin on the wells of a plastic plate, (b) incubation of the serum to be tested wit...
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| Veröffentlicht in: | Journal of immunological methods 1985-11, Vol.83 (2), p.309-316 |
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| container_issue | 2 |
| container_start_page | 309 |
| container_title | Journal of immunological methods |
| container_volume | 83 |
| creator | Cǎlugǎru, Ana Moraru, Ioan Zamfir, Genţiana Mǎrgineanu, Ilca |
| description | An antigen-coated plate radioimmunoassay used to detect autoantibodies of IgG and IgM classes against human serum albumin is described. It comprises 3 steps: (a) adsorption of glutaraldehyde-polymerized human serum albumin on the wells of a plastic plate, (b) incubation of the serum to be tested with the antigen in the wells, and (c) the addition of radiolabelled antibodies against human IgG or IgM.
The method has been used to estimate the level of anti-albumin autoantibodies (AAA) belonging to IgG or IgM classes in the sera of normal subjects and patients with liver diseases. |
| doi_str_mv | 10.1016/0022-1759(85)90252-2 |
| format | Article |
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The method has been used to estimate the level of anti-albumin autoantibodies (AAA) belonging to IgG or IgM classes in the sera of normal subjects and patients with liver diseases.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(85)90252-2</identifier><identifier>PMID: 4056407</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>anti-albumin autoantibody ; Autoantibodies - analysis ; class specific ; Cross Reactions ; Hepatitis, Chronic - immunology ; Hepatitis, Viral, Human - immunology ; Humans ; Immunoglobulin G - analysis ; Immunoglobulin M - analysis ; Liver Cirrhosis - immunology ; Radioimmunoassay - methods ; radiometric estimation ; Serum Albumin - immunology</subject><ispartof>Journal of immunological methods, 1985-11, Vol.83 (2), p.309-316</ispartof><rights>1985</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-2bf998d3e6e5cb23931274b2dfb149082266c5531b6fc822699a26add95b7c7f3</citedby><cites>FETCH-LOGICAL-c357t-2bf998d3e6e5cb23931274b2dfb149082266c5531b6fc822699a26add95b7c7f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0022175985902522$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4056407$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cǎlugǎru, Ana</creatorcontrib><creatorcontrib>Moraru, Ioan</creatorcontrib><creatorcontrib>Zamfir, Genţiana</creatorcontrib><creatorcontrib>Mǎrgineanu, Ilca</creatorcontrib><title>Radioimmunoassay of IgG and IgM anti-albumin autoantibodies in human serum</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>An antigen-coated plate radioimmunoassay used to detect autoantibodies of IgG and IgM classes against human serum albumin is described. It comprises 3 steps: (a) adsorption of glutaraldehyde-polymerized human serum albumin on the wells of a plastic plate, (b) incubation of the serum to be tested with the antigen in the wells, and (c) the addition of radiolabelled antibodies against human IgG or IgM.
The method has been used to estimate the level of anti-albumin autoantibodies (AAA) belonging to IgG or IgM classes in the sera of normal subjects and patients with liver diseases.</description><subject>anti-albumin autoantibody</subject><subject>Autoantibodies - analysis</subject><subject>class specific</subject><subject>Cross Reactions</subject><subject>Hepatitis, Chronic - immunology</subject><subject>Hepatitis, Viral, Human - immunology</subject><subject>Humans</subject><subject>Immunoglobulin G - analysis</subject><subject>Immunoglobulin M - analysis</subject><subject>Liver Cirrhosis - immunology</subject><subject>Radioimmunoassay - methods</subject><subject>radiometric estimation</subject><subject>Serum Albumin - immunology</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo67r6DxR6Ej1Uk7RJmosgix8rK4LoOeSrGmmbNWmE_fe27rJHD8PLzLwzwzwAnCJ4hSCi1xBinCNG-EVFLjnEBOd4D0xRxXDOOCT7YLqzHIKjGL8ghAhSOAGTEhJaQjYFT6_SOO_aNnVexijXma-zxcdDJjsz6POgvctlo1Lrukym3o8F5Y2zMRsqn6mVXRZtSO0xOKhlE-3JVmfg_f7ubf6YL18eFvPbZa4Lwvocq5rzyhSWWqIVLniBMCsVNrVCJYcVxpRqQgqkaK3HjHOJqTSGE8U0q4sZON_sXQX_nWzsReuitk0jO-tTFIyWBR5iMJYbow4-xmBrsQqulWEtEBQjQjHyESMfURHxh1DgYexsuz-p1prd0JbZ0L_Z9O3w5I-zQUTtbKetccHqXhjv_j_wC72kfxw</recordid><startdate>19851107</startdate><enddate>19851107</enddate><creator>Cǎlugǎru, Ana</creator><creator>Moraru, Ioan</creator><creator>Zamfir, Genţiana</creator><creator>Mǎrgineanu, Ilca</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19851107</creationdate><title>Radioimmunoassay of IgG and IgM anti-albumin autoantibodies in human serum</title><author>Cǎlugǎru, Ana ; Moraru, Ioan ; Zamfir, Genţiana ; Mǎrgineanu, Ilca</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-2bf998d3e6e5cb23931274b2dfb149082266c5531b6fc822699a26add95b7c7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>anti-albumin autoantibody</topic><topic>Autoantibodies - analysis</topic><topic>class specific</topic><topic>Cross Reactions</topic><topic>Hepatitis, Chronic - immunology</topic><topic>Hepatitis, Viral, Human - immunology</topic><topic>Humans</topic><topic>Immunoglobulin G - analysis</topic><topic>Immunoglobulin M - analysis</topic><topic>Liver Cirrhosis - immunology</topic><topic>Radioimmunoassay - methods</topic><topic>radiometric estimation</topic><topic>Serum Albumin - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cǎlugǎru, Ana</creatorcontrib><creatorcontrib>Moraru, Ioan</creatorcontrib><creatorcontrib>Zamfir, Genţiana</creatorcontrib><creatorcontrib>Mǎrgineanu, Ilca</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cǎlugǎru, Ana</au><au>Moraru, Ioan</au><au>Zamfir, Genţiana</au><au>Mǎrgineanu, Ilca</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radioimmunoassay of IgG and IgM anti-albumin autoantibodies in human serum</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1985-11-07</date><risdate>1985</risdate><volume>83</volume><issue>2</issue><spage>309</spage><epage>316</epage><pages>309-316</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><abstract>An antigen-coated plate radioimmunoassay used to detect autoantibodies of IgG and IgM classes against human serum albumin is described. It comprises 3 steps: (a) adsorption of glutaraldehyde-polymerized human serum albumin on the wells of a plastic plate, (b) incubation of the serum to be tested with the antigen in the wells, and (c) the addition of radiolabelled antibodies against human IgG or IgM.
The method has been used to estimate the level of anti-albumin autoantibodies (AAA) belonging to IgG or IgM classes in the sera of normal subjects and patients with liver diseases.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>4056407</pmid><doi>10.1016/0022-1759(85)90252-2</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1759 |
| ispartof | Journal of immunological methods, 1985-11, Vol.83 (2), p.309-316 |
| issn | 0022-1759 1872-7905 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | anti-albumin autoantibody Autoantibodies - analysis class specific Cross Reactions Hepatitis, Chronic - immunology Hepatitis, Viral, Human - immunology Humans Immunoglobulin G - analysis Immunoglobulin M - analysis Liver Cirrhosis - immunology Radioimmunoassay - methods radiometric estimation Serum Albumin - immunology |
| title | Radioimmunoassay of IgG and IgM anti-albumin autoantibodies in human serum |
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