Effect of aminophylline on calcium transport across the rat diaphragm
1. 1.|Calcium accumulation in muscle strips isolated from rat diaphragm reached steady state after 90 min with intracellular to extracellular ratio (IN/OUT) of 3.0. 2. 2.|Calcium uptake by rat diaphragm is a saturable and carrier mediated process that is significantly enhanced in the presence of ami...
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| Veröffentlicht in: | General pharmacology 1985, Vol.16 (5), p.541-543 |
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| container_end_page | 543 |
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| container_issue | 5 |
| container_start_page | 541 |
| container_title | General pharmacology |
| container_volume | 16 |
| creator | Saadeh, Ghandi M. Ayash, Raja E. Saadeh, Fadi M. Nassar, Camille F. |
| description | 1.
1.|Calcium accumulation in muscle strips isolated from rat diaphragm reached steady state after 90 min with intracellular to extracellular ratio (IN/OUT) of 3.0.
2.
2.|Calcium uptake by rat diaphragm is a saturable and carrier mediated process that is significantly enhanced in the presence of aminophylline in a dose-dependent pattern that produced maximal effect at 18.75 μg/ml.
3.
3.|A Lineweaver-Burk plot shows
J
max = 25
μM/hr per g dry wt and
K
t = 3.19 mM for the control, and
J
max = 26
μM/hr per g dry wt and
K
t = 2.27 mM in the presence of 18.75 μg/ml aminophylline.
4.
4.|Aminophylline had no effect on cell volume and water of the diaphragmatic cells. |
| doi_str_mv | 10.1016/0306-3623(85)90021-7 |
| format | Article |
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1.|Calcium accumulation in muscle strips isolated from rat diaphragm reached steady state after 90 min with intracellular to extracellular ratio (IN/OUT) of 3.0.
2.
2.|Calcium uptake by rat diaphragm is a saturable and carrier mediated process that is significantly enhanced in the presence of aminophylline in a dose-dependent pattern that produced maximal effect at 18.75 μg/ml.
3.
3.|A Lineweaver-Burk plot shows
J
max = 25
μM/hr per g dry wt and
K
t = 3.19 mM for the control, and
J
max = 26
μM/hr per g dry wt and
K
t = 2.27 mM in the presence of 18.75 μg/ml aminophylline.
4.
4.|Aminophylline had no effect on cell volume and water of the diaphragmatic cells.</description><identifier>ISSN: 0306-3623</identifier><identifier>EISSN: 1879-0011</identifier><identifier>DOI: 10.1016/0306-3623(85)90021-7</identifier><identifier>PMID: 4054581</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Aminophylline - pharmacology ; Animals ; Biological Transport - drug effects ; Body Water - drug effects ; Calcium - metabolism ; Diaphragm - drug effects ; Diaphragm - metabolism ; Female ; In Vitro Techniques ; Intracellular Fluid - drug effects ; Male ; Rats ; Rats, Inbred Strains ; Time Factors</subject><ispartof>General pharmacology, 1985, Vol.16 (5), p.541-543</ispartof><rights>1985</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-20ea865314ab203df42496776bfc76e8c8bc0466d2088dfb9f9281d7bb50c5563</citedby><cites>FETCH-LOGICAL-c357t-20ea865314ab203df42496776bfc76e8c8bc0466d2088dfb9f9281d7bb50c5563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4054581$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saadeh, Ghandi M.</creatorcontrib><creatorcontrib>Ayash, Raja E.</creatorcontrib><creatorcontrib>Saadeh, Fadi M.</creatorcontrib><creatorcontrib>Nassar, Camille F.</creatorcontrib><title>Effect of aminophylline on calcium transport across the rat diaphragm</title><title>General pharmacology</title><addtitle>Gen Pharmacol</addtitle><description>1.
1.|Calcium accumulation in muscle strips isolated from rat diaphragm reached steady state after 90 min with intracellular to extracellular ratio (IN/OUT) of 3.0.
2.
2.|Calcium uptake by rat diaphragm is a saturable and carrier mediated process that is significantly enhanced in the presence of aminophylline in a dose-dependent pattern that produced maximal effect at 18.75 μg/ml.
3.
3.|A Lineweaver-Burk plot shows
J
max = 25
μM/hr per g dry wt and
K
t = 3.19 mM for the control, and
J
max = 26
μM/hr per g dry wt and
K
t = 2.27 mM in the presence of 18.75 μg/ml aminophylline.
4.
4.|Aminophylline had no effect on cell volume and water of the diaphragmatic cells.</description><subject>Aminophylline - pharmacology</subject><subject>Animals</subject><subject>Biological Transport - drug effects</subject><subject>Body Water - drug effects</subject><subject>Calcium - metabolism</subject><subject>Diaphragm - drug effects</subject><subject>Diaphragm - metabolism</subject><subject>Female</subject><subject>In Vitro Techniques</subject><subject>Intracellular Fluid - drug effects</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Time Factors</subject><issn>0306-3623</issn><issn>1879-0011</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kElLxDAUx4Mo4zj6DRRyEj1UkzRbL4IM4wIDXvQc0ixOpG1q0hHm29tZ8OjpHf7Le-8HwCVGdxhhfo9KxIuSk_JGstsKIYILcQSmWIqqQAjjYzD9s5yCs5y_0GhihEzAhCJGmcRTsFh478wAo4e6DV3sV5umCZ2DsYNGNyasWzgk3eU-pgFqk2LOcFg5mPQAbdD9KunP9hyceN1kd3GYM_DxtHifvxTLt-fX-eOyMCUTQ0GQ05KzElNdE1RaTwmtuBC89kZwJ42sDaKcW4KktL6ufEUktqKuGTKM8XIGrve9fYrfa5cH1YZsXNPozsV1VoJTUomKjEa6N-4OTs6rPoVWp43CSG3pqS0atUWjJFM7ekqMsatD_7punf0LHXCN-sNed-OTP8EllU1wnXE2pJGisjH8v-AXWQN9sQ</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>Saadeh, Ghandi M.</creator><creator>Ayash, Raja E.</creator><creator>Saadeh, Fadi M.</creator><creator>Nassar, Camille F.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1985</creationdate><title>Effect of aminophylline on calcium transport across the rat diaphragm</title><author>Saadeh, Ghandi M. ; Ayash, Raja E. ; Saadeh, Fadi M. ; Nassar, Camille F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-20ea865314ab203df42496776bfc76e8c8bc0466d2088dfb9f9281d7bb50c5563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Aminophylline - pharmacology</topic><topic>Animals</topic><topic>Biological Transport - drug effects</topic><topic>Body Water - drug effects</topic><topic>Calcium - metabolism</topic><topic>Diaphragm - drug effects</topic><topic>Diaphragm - metabolism</topic><topic>Female</topic><topic>In Vitro Techniques</topic><topic>Intracellular Fluid - drug effects</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Time Factors</topic><toplevel>online_resources</toplevel><creatorcontrib>Saadeh, Ghandi M.</creatorcontrib><creatorcontrib>Ayash, Raja E.</creatorcontrib><creatorcontrib>Saadeh, Fadi M.</creatorcontrib><creatorcontrib>Nassar, Camille F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>General pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saadeh, Ghandi M.</au><au>Ayash, Raja E.</au><au>Saadeh, Fadi M.</au><au>Nassar, Camille F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of aminophylline on calcium transport across the rat diaphragm</atitle><jtitle>General pharmacology</jtitle><addtitle>Gen Pharmacol</addtitle><date>1985</date><risdate>1985</risdate><volume>16</volume><issue>5</issue><spage>541</spage><epage>543</epage><pages>541-543</pages><issn>0306-3623</issn><eissn>1879-0011</eissn><abstract>1.
1.|Calcium accumulation in muscle strips isolated from rat diaphragm reached steady state after 90 min with intracellular to extracellular ratio (IN/OUT) of 3.0.
2.
2.|Calcium uptake by rat diaphragm is a saturable and carrier mediated process that is significantly enhanced in the presence of aminophylline in a dose-dependent pattern that produced maximal effect at 18.75 μg/ml.
3.
3.|A Lineweaver-Burk plot shows
J
max = 25
μM/hr per g dry wt and
K
t = 3.19 mM for the control, and
J
max = 26
μM/hr per g dry wt and
K
t = 2.27 mM in the presence of 18.75 μg/ml aminophylline.
4.
4.|Aminophylline had no effect on cell volume and water of the diaphragmatic cells.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>4054581</pmid><doi>10.1016/0306-3623(85)90021-7</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0306-3623 |
| ispartof | General pharmacology, 1985, Vol.16 (5), p.541-543 |
| issn | 0306-3623 1879-0011 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76429792 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Aminophylline - pharmacology Animals Biological Transport - drug effects Body Water - drug effects Calcium - metabolism Diaphragm - drug effects Diaphragm - metabolism Female In Vitro Techniques Intracellular Fluid - drug effects Male Rats Rats, Inbred Strains Time Factors |
| title | Effect of aminophylline on calcium transport across the rat diaphragm |
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