Structural studies of the capsular polysaccharide of Acinetobacter calcoaceticus BD4
Compositional analysis of the intact and carboxyl‐reduced capsular polysaccharide of Acinetobacter calcoaceticus BD4 (PS‐4) showed it to consist of l‐rhamnose, d‐glucose, d‐glucuronic and d‐mannose in molar ratios of 4:1:1:1. 13C‐nuclear magnetic resonance spectroscopy, methylation analysis, oligosa...
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| Veröffentlicht in: | European journal of biochemistry 1985-10, Vol.152 (2), p.453-458 |
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| container_title | European journal of biochemistry |
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| creator | KAPLAN, Nachum ROSENBERG, Eugene JANN, Barbara JANN, Klaus |
| description | Compositional analysis of the intact and carboxyl‐reduced capsular polysaccharide of Acinetobacter calcoaceticus BD4 (PS‐4) showed it to consist of l‐rhamnose, d‐glucose, d‐glucuronic and d‐mannose in molar ratios of 4:1:1:1. 13C‐nuclear magnetic resonance spectroscopy, methylation analysis, oligosaccharide analysis and base‐catalyzed β‐elimination were used to elucidate the primary structure.
Oligosaccharides were obtained by enzymatic depolymerization with a specific bacteriophage‐induced depolymerase and by partial acid hydrolysis. From the results it is concluded that PS‐4 consists of repeating units of the heptasaccharide
The bacteriophage‐induced depolymerase was found to be an endo‐β‐d‐glucosidase that hydrolyzed the bond β‐d‐Glc‐(1→3)‐l‐Rha to generate a heptasaccharide in 40% yield. |
| doi_str_mv | 10.1111/j.1432-1033.1985.tb09218.x |
| format | Article |
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Oligosaccharides were obtained by enzymatic depolymerization with a specific bacteriophage‐induced depolymerase and by partial acid hydrolysis. From the results it is concluded that PS‐4 consists of repeating units of the heptasaccharide
The bacteriophage‐induced depolymerase was found to be an endo‐β‐d‐glucosidase that hydrolyzed the bond β‐d‐Glc‐(1→3)‐l‐Rha to generate a heptasaccharide in 40% yield.</description><identifier>ISSN: 0014-2956</identifier><identifier>EISSN: 1432-1033</identifier><identifier>DOI: 10.1111/j.1432-1033.1985.tb09218.x</identifier><identifier>PMID: 4054115</identifier><identifier>CODEN: EJBCAI</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acinetobacter - immunology ; Bacteriology ; Biological and medical sciences ; Catalysis ; Chemical Phenomena ; Chemistry ; Fundamental and applied biological sciences. Psychology ; Hydrolysis ; Magnetic Resonance Spectroscopy ; Methylation ; Microbiology ; Morphology, structure, chemical composition ; Oligosaccharides - analysis ; Oxidation-Reduction ; Polysaccharides, Bacterial - analysis ; Rhamnose - analysis</subject><ispartof>European journal of biochemistry, 1985-10, Vol.152 (2), p.453-458</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5173-9e30841a862c483f37c1d0a5b69c8aa035cbcae42905d90aefbf1da860fbe8d93</citedby><cites>FETCH-LOGICAL-c5173-9e30841a862c483f37c1d0a5b69c8aa035cbcae42905d90aefbf1da860fbe8d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8429663$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4054115$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KAPLAN, Nachum</creatorcontrib><creatorcontrib>ROSENBERG, Eugene</creatorcontrib><creatorcontrib>JANN, Barbara</creatorcontrib><creatorcontrib>JANN, Klaus</creatorcontrib><title>Structural studies of the capsular polysaccharide of Acinetobacter calcoaceticus BD4</title><title>European journal of biochemistry</title><addtitle>Eur J Biochem</addtitle><description>Compositional analysis of the intact and carboxyl‐reduced capsular polysaccharide of Acinetobacter calcoaceticus BD4 (PS‐4) showed it to consist of l‐rhamnose, d‐glucose, d‐glucuronic and d‐mannose in molar ratios of 4:1:1:1. 13C‐nuclear magnetic resonance spectroscopy, methylation analysis, oligosaccharide analysis and base‐catalyzed β‐elimination were used to elucidate the primary structure.
Oligosaccharides were obtained by enzymatic depolymerization with a specific bacteriophage‐induced depolymerase and by partial acid hydrolysis. From the results it is concluded that PS‐4 consists of repeating units of the heptasaccharide
The bacteriophage‐induced depolymerase was found to be an endo‐β‐d‐glucosidase that hydrolyzed the bond β‐d‐Glc‐(1→3)‐l‐Rha to generate a heptasaccharide in 40% yield.</description><subject>Acinetobacter - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Catalysis</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolysis</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Methylation</subject><subject>Microbiology</subject><subject>Morphology, structure, chemical composition</subject><subject>Oligosaccharides - analysis</subject><subject>Oxidation-Reduction</subject><subject>Polysaccharides, Bacterial - analysis</subject><subject>Rhamnose - analysis</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkE1r3DAQhkVISbdpfkLBhJCbXcmSvFIuIZ9tIdBD0rMYj8fEi3e9kWSS_feRWbP36jKH95nRy8PYueCFSO_nqhBKlrngUhbCGl3EmttSmOLjiC0O0TFbcC5UXlpdfWXfQlhxzitbLU_YieJaCaEX7OU5-hHj6KHPQhybjkI2tFl8pQxhG8YefLYd-l0AxFfwXUNTfIPdhuJQA0byCexxAKTY4Riy23v1nX1poQ90Ns9T9u_x4eXud_7099efu5unHLVYytyS5EYJMFWJyshWLlE0HHRdWTQAXGqsEUiVluvGcqC2bkWTcN7WZBorT9nl_u7WD28jhejWXUDqe9jQMAa3rFRpyqpK4NUeRD-E4Kl1W9-twe-c4G5S6lZu8uYmb25S6mal7iMt_5h_Ges1NYfV2WHKL-YcQlLRethgFw6YSf1ThYRd77H3rqfdfxRwjw-3z0pL-QmVZZRO</recordid><startdate>198510</startdate><enddate>198510</enddate><creator>KAPLAN, Nachum</creator><creator>ROSENBERG, Eugene</creator><creator>JANN, Barbara</creator><creator>JANN, Klaus</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198510</creationdate><title>Structural studies of the capsular polysaccharide of Acinetobacter calcoaceticus BD4</title><author>KAPLAN, Nachum ; ROSENBERG, Eugene ; JANN, Barbara ; JANN, Klaus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5173-9e30841a862c483f37c1d0a5b69c8aa035cbcae42905d90aefbf1da860fbe8d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Acinetobacter - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Catalysis</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolysis</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Methylation</topic><topic>Microbiology</topic><topic>Morphology, structure, chemical composition</topic><topic>Oligosaccharides - analysis</topic><topic>Oxidation-Reduction</topic><topic>Polysaccharides, Bacterial - analysis</topic><topic>Rhamnose - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KAPLAN, Nachum</creatorcontrib><creatorcontrib>ROSENBERG, Eugene</creatorcontrib><creatorcontrib>JANN, Barbara</creatorcontrib><creatorcontrib>JANN, Klaus</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KAPLAN, Nachum</au><au>ROSENBERG, Eugene</au><au>JANN, Barbara</au><au>JANN, Klaus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural studies of the capsular polysaccharide of Acinetobacter calcoaceticus BD4</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>1985-10</date><risdate>1985</risdate><volume>152</volume><issue>2</issue><spage>453</spage><epage>458</epage><pages>453-458</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><coden>EJBCAI</coden><abstract>Compositional analysis of the intact and carboxyl‐reduced capsular polysaccharide of Acinetobacter calcoaceticus BD4 (PS‐4) showed it to consist of l‐rhamnose, d‐glucose, d‐glucuronic and d‐mannose in molar ratios of 4:1:1:1. 13C‐nuclear magnetic resonance spectroscopy, methylation analysis, oligosaccharide analysis and base‐catalyzed β‐elimination were used to elucidate the primary structure.
Oligosaccharides were obtained by enzymatic depolymerization with a specific bacteriophage‐induced depolymerase and by partial acid hydrolysis. From the results it is concluded that PS‐4 consists of repeating units of the heptasaccharide
The bacteriophage‐induced depolymerase was found to be an endo‐β‐d‐glucosidase that hydrolyzed the bond β‐d‐Glc‐(1→3)‐l‐Rha to generate a heptasaccharide in 40% yield.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>4054115</pmid><doi>10.1111/j.1432-1033.1985.tb09218.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-2956 |
| ispartof | European journal of biochemistry, 1985-10, Vol.152 (2), p.453-458 |
| issn | 0014-2956 1432-1033 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76428266 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Acinetobacter - immunology Bacteriology Biological and medical sciences Catalysis Chemical Phenomena Chemistry Fundamental and applied biological sciences. Psychology Hydrolysis Magnetic Resonance Spectroscopy Methylation Microbiology Morphology, structure, chemical composition Oligosaccharides - analysis Oxidation-Reduction Polysaccharides, Bacterial - analysis Rhamnose - analysis |
| title | Structural studies of the capsular polysaccharide of Acinetobacter calcoaceticus BD4 |
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